| 王伟,方军,李化壮,等.富马酸二甲酯对大鼠椎间盘退变髓核中低氧诱导因子-1α表达和功能的影响[J].安徽医药,2017,21(2):226-230. |
| 富马酸二甲酯对大鼠椎间盘退变髓核中低氧诱导因子-1α表达和功能的影响 |
| Experimental research on the effect ofdimethyl fumarate on the expression and function of hypoxia-inducible factor-1α on rat intervertebral disc degeneration |
| 投稿时间：2016-06-21 |
| DOI： |
| 中文关键词: 椎间盘退变 富马酸二甲酯 低氧诱导因子-1α |
| 英文关键词: Lumbar degenerative intervertebral disc Dimethyl fumarate Hypoxia-inducible factor-1α |
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| 摘要点击次数: 2468 |
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| 中文摘要: |
| 目的 建立SD大鼠腰椎间盘退变模型采用测定低氧诱导因子-1α(HIF-1α)在大鼠模型腰椎间盘髓核中的表达情况及作用,探讨富马酸二甲酯(DMF)对其表达及功能的影响。方法 对大鼠腰椎退变模型行磁共振成像(MRI)扫描后,取L4/L5节段髓核组织行蛋白多糖检测,石蜡包埋、切片后进行HIF-1α的免疫组化染色。结果 MRI结果显示模型对照组(B组)的椎间盘髓核存在明显的退变,T2加权相信号降低 (P<0.05)。B组髓核中的HIF-1α呈强阳性,与另两组比较差异有统计学意义(P<0.05)。对全部的髓核组织的进行蛋白多糖含量的检测,可见对照组B组的蛋白多糖成分(2.06±0.04) ng·g-1,流失最为严重,富马酸二甲酯用药组(C组)(2.06±0.05) ng·g-1相对较轻,正常对照组(A组)含量为(3.28±0.03) ng·g-1,三组比较差异有统计学意义(P<0.01)。结论 HIF-1α在退变的腰椎间盘组织中表达增高,其表达与腰椎的退变密切相关。富马酸二甲酯作为HIF-1α的抑制剂能降低HIF-1α的表达,抑制和延缓腰椎间盘退变的形成和发展。 |
| 英文摘要: |
| Objective To get experimental lumbar degenerative intervertebral disc model and evaluate the expression of HIF-1α and the affect of dimethyl fumarate (DMF) on the expression and function of HIF-1α. Methods The L4/L5 segment nucleus pulposus tissue was taken for detection of protein polysaccharides and the immunohistochemistry with HIF-1α after MRI scan of the rat model of lumbar degeneration. Results The results of MRI showed that there was a significant degeneration of the intervertebral disc nucleus in the group B,and the T2 weighted phase signal was decreased (P<0.05).The immunohistochemistry showed that HIF-1α was strongly positive in the nucleus of group C,the differences were statistically significant (P<0.05) compared with two control groups.The proteoglycan content in the nucleus pulposus was of all groups shown that proteoglycan contents in group A,B,C was (3.28±0.03),(2.06±0.04),(2.06±0.05) ng·g-1 respectively.The difference between them was statistically significant (P<0.01). Conclusions The high expression of HIF-1α in lumbar degenerative disc indicated that the expression of HIF-1α is closely related to the lumbar disc degenerative.Treatment with Dimethyl Fumarate inhibits the expression of HIF-1α and the development of experimental rat lumbar degenerative disc in vivo. |
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