| 陈兴华,黄珍谷,王棚,等.葛根素通过调控炎性因子抑制兔膝关节骨性关节炎的研究[J].安徽医药,2017,21(2):230-234. |
| 葛根素通过调控炎性因子抑制兔膝关节骨性关节炎的研究 |
| Puerarin inhibits the progress of osteoarthritis of rabbit knee by balancing inflammation system |
| 投稿时间：2016-10-22 |
| DOI： |
| 中文关键词: 葛根素 骨性关节炎 炎性因子 基质金属蛋白酶 Ⅱ型胶原蛋白 |
| 英文关键词: Puerarin Osteoarthritis Inflammatory factor Matrix metalloproteinases Collagen Ⅱ |
| 基金项目:重庆市卫生局科研项目(2012-2-356) |
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| 中文摘要: |
| 目的 研究葛根素对兔膝关节骨性关节炎的抑制作用及机制。方法 将40只成年新西兰兔随机分为正常组、模型组、生理盐水组、葛根素组,每组10只,后三组构建兔膝关节骨性关节炎模型。模型构建成功后,生理盐水组和葛根素组分别膝关节内注射生理盐水、葛根素0.5 mL,5周共10次。药物注射完成后,切开膝关节肉眼观察关节面,抽取关节液采用ELISA法检测白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)、肿瘤坏死因子α(TNF-α)、环氧化酶2(COX2)、前列腺素E2(PGE2)表达水平,Western blot 法检测关节软骨组织中基质金属蛋白酶2(MMP-2)、Ⅱ型胶原蛋白(Collagen Ⅱ)表达水平。结果 葛根素作用后膝关节软骨面病损程度明显减轻。葛根素作用后膝关节液中IL-1β、TNF-α、COX2、 PGE2表达水平降低,IL-10表达水平增加,而关节软骨组织中MMP-2表达降低,Collagen Ⅱ表达增加。结论 葛根素可能参与了骨性关节炎中促炎因子与抑炎因子间的平衡,具有一定的抑制细胞外基质中胶原的降解作用,从而有可能减缓骨性关节炎的病程进展。 |
| 英文摘要: |
| Objective To investigate the effect of puerarin on osteoarthritis of rabbit knee and its mechanism.Methods Forty adult new zealand rabbits were randomly divided into normal,model,saline and puerarin group,ten in each group.The rabbits in last three groups were chose to construct knee osteoarthritis model.After success of model construction,the saline and puerarin group were injected with 0.5 mL saline or puerarin into knee joint cavity respectively,twice one week for five weeks.After treatment,changes on knee joint articular surface was observed by naked eye after incision.The expression level of IL-1β,IL-10,TNF-α,COX2,PGE2 in synovia were detected by ELISA,and the expression level of MMP-2 and Collagen Ⅱ in articular cartilage tissue were detected by Western blot.Results Cartilage lesion were alleviated after treatment with puerarin.The expression of IL-1β,TNF-α,COX2,PGE2 decreased an IL-10 increased in synovia.MMP-2 expression decreased and Collagen Ⅱ increased in articular cartilage tissue.Conclusion Puerarin by inhibiting degradation of collagen in extracellular matrix,may be involved in the balance between proinflammatory cytokines and anti-inflammatory factors,and prevent the progression of osteoarthritis. |
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