| 苏涌,杨春兰,黄燕,等.高效液相色谱法测定血浆中伏立康唑的浓度[J].安徽医药,2017,21(2):244-247. |
| 高效液相色谱法测定血浆中伏立康唑的浓度 |
| Determination of plasma concentration of voriconazole by HPLC |
| 投稿时间：2016-07-28 |
| DOI： |
| 中文关键词: 伏立康唑 治疗药物监测 高效液相色谱法 |
| 英文关键词: Voriconazole TDM HPLC |
| 基金项目: |
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| 摘要点击次数: 2325 |
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| 中文摘要: |
| 目的 建立适用于病人血浆中伏立康唑浓度测定的高效液相色谱法。方法 色谱柱为Hypersil ODS2 C18(4.6 mm×250 mm,5 μm)；流动相为乙腈∶水(36∶64)；流速为1.0 mL·min-1；检测波长为254 nm；柱温为30 ℃；内标为艾司唑仑。结果 伏立康唑及内标艾司唑仑的色谱峰分离良好,伏立康唑在0.06～20 mg·L-1范围内线性关系良好,r=0.999 9。日内及日间RSD在1.49%～2.91%之间,绝对回收率在87.07%～96.82%之间。检测15例使用伏立康唑的病人血浆药物浓度,其均值(4.99±4.77) mg·L-1。结论 该方法专属性强、灵敏度高,适合伏立康唑的血浆浓度测定。 |
| 英文摘要: |
| Objective To develop a HPLC method for the determination of voriconazole in Plasma.Methods Hypersil ODS2 C18(4.6 mm×250 mm,5 μm)column was used and the flow rate was 1.0 mL·min-1.The mobile phase was consisted of acetonitrile-water (36∶64).The column temperature was 30 ℃ and the detective wave length was 254 nm.Estazolam was selected as internal standard.Results A linearity of voriconazole concentration curve was between 0.06-20 mg·L-1,r= 0.999 9.The intra-day and inter-day RSDs were between 1.49%-2.91%,absolute recovery rates of voriconazole were between 87.07%-96.82%.By detecting plasma voriconazole concentration in 15 patients,the average plasma drug concentration was (4.99±4.77) mg·L-1.Conclusion This method was sensitive and specific,which is suitable for clinical therapeutic drug monitoring (TDM). |
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