| 张青松,曹立赢,王明君,等.5-甲基硫代腺苷对结肠癌Hct116细胞增殖的影响[J].安徽医药,2017,21(4):646-649. |
| 5-甲基硫代腺苷对结肠癌Hct116细胞增殖的影响 |
| Role of methylthioadenosine in cell proliferation ofcolon cancer Hct116 cells |
| 投稿时间：2016-07-16 |
| DOI： |
| 中文关键词: 5-甲基硫代腺苷结肠癌细胞增殖 RNA 聚合酶Ⅲ |
| 英文关键词: Methylthioadenosine Conlon cancer Cell proliferation RNA Pol Ⅲ |
| 基金项目:唐山市科技支撑项目(14130260)5-甲基硫代腺苷对结肠癌Hct116细胞增殖的影响张青松,曹立赢,王明君,温英武,费乐学,高建超,郑景珍,李浩,戴世龙 (开滦总医院普外科,河北 唐山 063000)5-甲基硫代腺苷对结肠癌Hct116细胞增殖的影响张青松,曹立赢,王明君,温英武,费乐学,高建超,郑景珍,李浩,戴世龙 Role of methylthioadenosine in cell proliferation of colon cancer Hct116 cells ZHANG Qingsong,CAO Liying,WANG Mingjun,WEN Yingwu,FEILexue,GAO Jianchao,ZHENG Jingzhen,LI Hao,DAI Shilong |
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| 中文摘要: |
| 目的 研究5-甲基硫代腺苷(MTA)对结肠癌Hct116细胞增殖的影响及其相关机制。方法 采用MTT比色法及软琼脂克隆形成试验分析MTA对结肠癌Hct116细胞增殖的影响,采用Realtime PCR的方法分析RNA聚合酶Ⅲ依赖基因(tRNAs、5S rRNA)及TFⅢB亚单位TBP、Bdp1和Brf1基因mRNA水平的变化,并采用Western Blot的方法分析了TFⅢB亚单位Bdp1蛋白水平的变化。结果 MTA对结肠癌Hct116细胞具有明显的增殖抑制作用,在这一过程中,RNA聚合酶Ⅲ依赖基因(tRNAs、5S rRNA)的mRNA水平明显下调；同时TFⅢB亚单位Bdp1在mRNA水平和蛋白水平也出现了明显下调。结论 MTA对结肠癌Hct116细胞具有明显增殖抑制作用；RNA聚合酶Ⅲ依赖基因转录的下调、TFⅢB亚单位Bdp1蛋白的下调可能在这一抗肿瘤过程中起到重要作用。 |
| 英文摘要: |
| Objective To study the role and mechanism of methylthioadenosine in cell proliferation of colon cancer Hct116 cells.Methods MTT assay and anchorage-independent growth were used to detect the effects of methylthioadenosine on proliferation in Hct116 cells；the level of RNA Pol Ⅲ-dependent (tRNAs,5SrRNA) and the subunits of TFⅢB (TBP,Bdp1 and Brf1) transcription were detected with the method of Realtime PCR；the level of subunit of TFⅢB (Bdp1) protein was detected with the method of Western Blot.Results Methylthioadenosine could significantly inhibitthe proliferation of Hct116 cells;RNA Pol Ⅲ-dependent (tRNAs,5S rRNA)transcription was downregulated significantly;mRNA and protein of the Bdp1 were also downregulated significantly in this course.Conclusions Methylthioadenosine could inhibit significantly the proliferation ofHct116 cells and downregulate RNA Pol Ⅲ-dependent (tRNAs,5S rRNA)transcription and protein of the subunit of the TFⅢB Bdp1,which might play a major role in the treatment of cancer. |
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