文章摘要
庹敏,童仕伦.PTEN、PI3K和miR-21在胃癌中的表达及相关性研究[J].安徽医药,2017,21(11):2019-2022.
PTEN、PI3K和miR-21在胃癌中的表达及相关性研究
Expressions of PTEN,PI3K and miR-21 in gastric cancer and their correlations
投稿时间:2017-03-25  
DOI:
中文关键词: 胃癌  第10号染色体同源丢失性磷酸酶-张力蛋白基因  磷脂酰肌醇3-激酶  miR-21
英文关键词: Gastric cancer  PTEN  PI3K  miR-21
基金项目:
作者单位E-mail
庹敏 武汉大学人民医院胃肠外科,湖北 武汉 430060  
童仕伦 武汉大学人民医院胃肠外科,湖北 武汉 430060 tongshilun@163.com 
摘要点击次数: 2008
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中文摘要:
      目的 探讨第10号染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)、磷脂酰肌醇3-激酶(PI3K)和miR-21在胃癌中的表达及相互关系。方法 收集77例胃癌患者手术切除的胃癌组织及胃黏膜组织标本,应用荧光定量RT-PCR及Western blotting技术检测miR-21、PTEN和PI3K蛋白的表达水平,并分析miR-21与PTEN/PI3K信号通路之间的关系,以及与临床病理参数之间的相关性。结果 胃癌组织中miR-21及PI3K蛋白的表达水平均明显高于胃黏膜组织(P<0.05),PTEN蛋白的表达水平则明显降低(P<0.05);胃癌组织中PTEN蛋白的表达与肿瘤浸润深度、分化、TNM分期、淋巴转移有关(P<0.05),PI3K蛋白的表达与肿瘤淋巴转移和TNM分期有关(P<0.05);胃癌组织中PTEN蛋白的相对表达水平与miR-21和PI3K蛋白表达呈负相关(r=-0.428,P<0.05;r=-0.517,P<0.05)。结论 miR-21可能通过抑制PTEN,激活P13K信号通路参与胃癌的发生发展。
英文摘要:
      Objective To investigate the expressions of phosphatase and tensin homologue deleted on chromosome 10(PTEN),phosphatidylinositol 3-kinase(PI3K) and miR-21 in gastric cancer(GC) and normal gastric tissues and their correlation. Methods Using Western blotting and real-time quantitative polymerase chain reaction(RT-qPCR),we examined the expression of PTEN,PI3K and miR-21 in 77 cases of gastric cancer tissues and paired normal gastric tissues. Results Compared withnormal gastric tissues,the gastric cancer tissues showed a significantly higher expression of miR-21(t=16.768 5,P<0.05) and PI3K(t=11.399 1,P<0.05),but significantly lower PTEN expression(t=18.381 3,P<0.05).The expression of PTEN protein in gastric cancer was highly correlated with the degree of differentiation,invasion depth,lymph node metastasis and TNM stage(P<0.05).PI3K protein expression was associated with TNM stage and lymph node metastasis(P<0.05).PTEN protein expression was negatively correlated with the expression of miR-21(r=-0.428,P<0.05) and PI3K protein(r=-0.517,P<0.05) in gastric cancer tissues. Conclusions miR-21 promotes gastric carcinogenesis via inhibiting PTEN expression which in turn upregulating the expression of P13K in gastric cancer cells.
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