丁苯酞对db/db小鼠学习记忆及海马NR2B受体的影响

王海芳; 张钢; 张彦春; 董运强; 赵娜; 兰敏; 冀素晓; 张松筠

文章摘要

王海芳,张钢,张彦春,等.丁苯酞对db/db小鼠学习记忆及海马NR2B受体的影响[J].安徽医药,2017,21(12):2149-2151.

丁苯酞对db/db小鼠学习记忆及海马NR2B受体的影响

Effect of L-3-n-butylphthalide on learning,memory and expression of NR2B receptors in hippocampus of db/db miceWANG Haifang¹,ZHANG Gang¹,ZHANG Yanchun²,DONG Yunqiang³,ZHAO Na¹,LAN Min¹,JI Suxiao¹,ZHANG Songyun⁴ (1.Department of Endocrinology,The First Hospital of Handan,Handan,Hebei 056000,China; 2.Department of Endocrinology,Department of Handan Central Hospital,Handan,Hebei 056000,China;3.Handan Municipal Center for Disease Control and Prevention,Handan,Hebei 056000,China; 4.Department of Endocrinology,Second Hospital of Hebei Medical University,Shijiazhuang,Hebei 050000,China) Abstract:Objective

投稿时间：2017-03-15

DOI：

中文关键词: 丁苯酞糖尿病认知功能障碍 db/db小鼠 N-甲基-D-天冬氨酸受体2B亚基

英文关键词: L-NBP Diabetes mellitus Cognitive dysfunction db/db mice N-methyl-D-aspartate receptor 2B subunit

基金项目:邯郸市科学技术研究与发展计划项目(1623208064)

作者	单位	E-mail
王海芳	邯郸市第一医院内分泌一科,河北邯郸 056000
张钢	邯郸市第一医院内分泌一科,河北邯郸 056000
张彦春	邯郸市中心医院分院内分泌科, 河北邯郸 056000
董运强	邯郸市疾病预防控制中心,河北邯郸 056000
赵娜	邯郸市第一医院内分泌一科,河北邯郸 056000
兰敏	邯郸市第一医院内分泌一科,河北邯郸 056000
冀素晓	邯郸市第一医院内分泌一科,河北邯郸 056000
张松筠	河北医科大学第二医院内分泌科,河北石家庄 050000	zhangsongyun2016@126.com

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中文摘要:

目的观察丁苯酞对2型糖尿病db/db小鼠学习记忆及海马N-甲基-D-天冬氨酸受体2B亚基(NR2B)表达的影响,探讨丁苯酞改善糖尿病认知功能障碍的机制。方法将16只db/db小鼠按随机数字表法分为丁苯酞干预组(L-NBP组,n=8)和糖尿病对照组(DM组,n=8),同窝出生的db/m小鼠作为正常对照组(NC组,n=10)。适应性喂养1周后,L-NBP组每日以溶于植物油中的丁苯酞灌胃,剂量为120 mg·kg-1,DM组与NC组则给予同等剂量的植物油灌胃,干预6周后,进行指标观察。应用Morris水迷宫检测小鼠的学习记忆能力。实时荧光定量PCR检测NR2B mRNA的表达情况。结果与NC组比较,DM组小鼠逃避潜伏期延长和平均探索次数减少(P<0.05),而L-NBP组小鼠较DM组上述学习记忆成绩明显改善(P<0.05)；与NC组相比,DM组和L-NBP组小鼠海马CA1区 NR2B mRNA表达水平明显下降(P<0.05),而L-NBP组较DM组NR2B mRNA表达水平明显增高(P<0.05)。结论丁苯酞可改善糖尿病引发的认知功能障碍,推测其机制可能与上调海马NR2B的表达有关。

英文摘要:

Objective To study the effect of L-3-n-butylphthalide (L-NBP) on learning,memory and expression of N-Methyl-D-Aspartate (NMDA) receptor NR2B subunit of db/db mice and its possible mechanism. Methods Sixteen diabetic db/db mice were randomly assigned into two groups by random number table:L-NBP group (n=8) and DM group(n=8).Littermate db/m mice (NC group,n=10) were used as normal control.After one week of adaptive feeding the mice in L-NBP group were given L-NBP dissolved in vegetable oil by oral gavage once a day at a dose of 120 mg·kg-1 body weight,while DM and NC groups were both treated with equal amount of vegetable oil for 6 weeks.After 6 weeks of intervention,all the indexes were observed.The learning and memory ability of the mice was detected by Morris water maze test.The expression of NR2B mRNA was detected by realtime polymerase chain reaction(RT-PCR). Results The escaping latency time(s) was longer and the average number of cross platform exploration was less in DM group than in NC group (P<0.05).The learning and memory scores were significantly higher in L-NBP group than in DM group (P<0.05).The expression levels of NR2B mRNA in hippocampal CA1 area were significantly lower in DM and L-NBP groups than in NC group (P<0.05).As compared with the mice in DM group,the expression levels of NR2B mRNA in hippocampal area were significantly higher in L-NBP group (P<0.05). Conclusions L-NBP can improve the learning and memory of db/db mice by up-regulating the expression of NR2B in hippocarnpal CA1 area.

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