| 赵荣,肖会敏,何悦,等.调经益母片的高效液相色谱指纹图谱及成分定量研究[J].安徽医药,2018,22(7):1263-1267. |
| 调经益母片的高效液相色谱指纹图谱及成分定量研究 |
| Study on fingerprint of Tiaojingyimu tablet and quantitative analysis of components by HPLC |
| 投稿时间：2016-11-07 |
| DOI： |
| 中文关键词: 色谱法, 高压液相 丹参素 益母草碱 迷迭香酸 丹酚酸B |
| 英文关键词: Chromatography, high pressure liquid Danshensu Leonurine Rosmarinic acid Salvianolic acid B |
| 基金项目: |
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| 摘要点击次数: 2065 |
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| 中文摘要: |
| 目的 研究调经益母片的高效液相色谱(HPLC)法指纹图谱,并测定丹参素、益母草碱、迷迭香酸与丹酚酸B的含量。方法 采用HPLC法,色谱条件:阿克苏诺贝尔Kromasil C18柱(250 mm×4.6 mm, 5.0 μm)；流动相为乙腈-5.0 g·L-1磷酸水溶液,梯度洗脱；流速为0.8 mL·min-1；柱温为30 ℃；检测波长为270 nm。结果 通过中药色谱指纹图谱相似度评价系统(2004A),确定了调经益母片的HPLC指纹图谱共有模式,标定了43个共有峰,相似度为0.938~0.985；并测定了10批制剂中丹参素、益母草碱、迷迭香酸与丹酚酸B的平均含量分别为2.39、3.61、5.98、60.11 mg·g-1,RSD(%)分别为1.30、0.91、1.46、0.92%。结论 HPLC指纹图谱测定方法及其丹参素、益母草碱、迷迭香酸与丹酚酸B的定量方法简便易行、稳定可靠,可作为调经益母片制剂的质量控制方法。 |
| 英文摘要: |
| Objective To establish HPLC fingerprint method for quality control of Tiaojingyimu Tablet, and determine the content of tanshinol,leonurine,rosmarinic acid,salvianolic acid B. Methods The High Performance Liquid Chromatography (HPLC) analysis was performded on a Kromasil C18(250 mm×4.6 mm, 5μm)column.The mobile phase was a mixture of acetonitrile and 0.4% phosphoric acid solution in gradient elution. The flow rate was 0.8 mL·min-1, and the column temperature was set at 30 ℃, and the detection wavelength was set at 270 nm. Results Under the above chromatographic conditions, the characteristic fingerprint peak of Tiaojingyimu Tablet was composed of 43 chromatographic peaks through the chromatographic fingerprint similarity evaluation system (2004A) analysis. And the acquaintance degree was 0.938~0.985. And the average content of tanshinol, leonurine, rosmarinic acid, salvianolic acid Bwere respectively, 2.39,3.61,5.98,0.11 mg·g-1 and their RSDs(%) were , respectively, 1.30,0.91,1.46,0.92% in 10 batches of Tiaojingyimu Tablet. Conclusion The method is simple, accurate and has a good repeatability. The study provides scientific foundation for quality evaluation and quality control standards of Tiaojingyimu Tablet. |
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