| 刘倩倩,刘倩,李文涛,等.梓醇对阿尔茨海默病大鼠模型大脑皮质保护作用[J].安徽医药,2019,23(10):1934-1938. |
| 梓醇对阿尔茨海默病大鼠模型大脑皮质保护作用 |
| The protective effect of catalpol on cerebral cortex in an Alzheimer’s disease rat model |
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| DOI：10.3969/j.issn.1009?6469.2019.10.007 |
| 中文关键词: 阿尔茨海默病 梓醇 大脑皮质 受体，毒蕈碱 M1 迷宫学习 大鼠， Wistar |
| 英文关键词: Alzheimer disease Catalpol Cerebral cortex Receptor,muscarinic M1 Maze learning Rats,wistar |
| 基金项目:山东省自然科学基金项目( ZR2014HL106) |
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| 中文摘要: |
| 目的观察梓醇对阿尔茨海默病( AD)大鼠模型大脑皮质的保护作用。方法健康雄性 Wistar大鼠 36只，体质量范围为 250～300 g，随机数字表法分为 4组，每组 9只:健康对照组、模型组、梓醇小剂量组、梓醇大剂量组。除健康对照组大鼠外，其余组大鼠均行右侧脑室定位注射 β?淀粉样蛋白片段 25?35(Aβ25?35)和腹腔注射 D?半乳糖结合制备 AD模型，然后梓醇小剂量组大鼠注射梓醇 5 mg·kg-1·d-1，梓醇大剂量组大鼠注射梓醇 10 mg·kg-1·d-1，健康对照组和模型组大鼠注射等容积 0.9%氯化钠水溶液，连续腹腔注射给药 7d。Y?迷宫检测大鼠的学习指数，苏木精 ?伊红( HE)染色和透射电镜观察 AD大鼠皮层结构，免疫组织化学染色观察大脑皮质胆碱能毒蕈碱受体 M1亚型( M1受体)的表达，蛋白质印迹法( Western Blot)检测 M1受体蛋白的含量。结果实验过程中模型组大鼠死亡 1只，最终模型组 n＝8，其余组均 n＝9。与模型组比较，从造模后第 14天开始，梓醇显著提高了大鼠的学习指数(第 14天:小剂量组 t＝3.267，P＝0.030 9，大剂量组 t＝4.648，P＝0.009 7；第 21天:小剂量组 t＝5.010，P＝0.007 4，大剂量组 t＝4.614，P＝0.009 9)第 14天 4组大鼠的学习指数分别为( 5.6±0.2)(2.6±0.3)(3.7±0.5)(4.4±0.6)；第 21天 4组大鼠的学习指数各组分别为( 6.3±0.8)，，(4.1±0.5)，(5.3±0.4)，(5.8±0.8)。HE染色可，见模型组大，鼠大脑皮，质结构有损伤性表现，透射电镜检查见大脑皮质神经元细胞内线粒体、粗面内质网、核染色质等异常改变，梓醇干预组神经元的结构显著 改善。免疫组织化学染色可见模型组大鼠大脑皮质 M1受体染色浅，神经元数目减少， M1受体蛋白表达下调，梓醇能够显著上调 M1受体蛋白表达(与模型组比较，小剂量组 t＝3.983，P＝0.016 4；大剂量组 t＝6.694，P＝0.002 6)4组大鼠 M1受体蛋白表达分别为 100%，64.75%，74.63%，85.74%。结论梓醇能够改善 AD大鼠大脑皮质结构异常，升高 M1受体，表达。 |
| 英文摘要: |
| Objective To observe the protective effect of catalpol on cerebral cortex in an Alzheimer’s disease(AD)rat model. Methods Thirty?six male Wistar rats，weighing 250?300 g，were used and assigned into 4 groups according to random number ta? ble method，9 rats in each group.The groups included the normal control group，the model group，the low?dose catalpol group and the high?dose catalpol group.Except for the rats in normal control group，the rats in other groups were prepared by injection of Aβ25?35 in the right ventricle and D?galactose intraperitoneally.The low?dose catapol group was injected with 5 mg·kg-1·d-1 of cata? pol，and the high?dose catalpol group was injected with 10 mg·kg-1·d-1 of catapol.The normal control group and the model groupwere injected with the same volume of 0.9% sodium chloride solution intraperitoneally once a day for 7 days.Study index of ratswas observed by Y?maze test，cortex structure of AD rats observed by Hematoxylin?ehong(HE)staining and electronic microscope， expression of M1 subtype of muscarinic cholinergic receptors(M1 receptor) in cerebral cortex observed by immunohistochemical staining，and protein content of M1 receptor detected by Western Blot.Results A rat in the model group died during the study andthere were 8 rats left in the group while there were 9 rats in each of the other groups.Compared with the model group，the study in? dex of the rats increased significantly from the 14d(day 14:as for low?dose catalpol group t＝3.267，P＝0.030 9，as for high?dose catalpol group t＝4.648，P＝0.009 7；day 21:as for low?dose catalpol group t＝5.010，P＝0.007 4，as for high?dose catalpol group t＝4.614，P＝0.009 9).At day 14，the study indexes of rats in the 4 groups were(5.6±0.2)(2.6±0.3)(3.7±0.5)，(4.4±0.6)re? spectively，and the study indexes turned to(6.3±0.8)，(4.1±0.5)，(5.3±0.4)，(5.8±0.8)respec，tivelyatda，y 21.Abnormalities were found in cortex structure by HE staining，and ultrastructure changes of mitochondria，rough endoplasmic reticulum，nuclear chroma? tin were observed by transmission electron microscope，while the structure of the neuron was significantly improved in catalpolgroups.Immunohistochemical staining results showed that the expression of M1 receptor in the cerebral cortex of rats decreased，the number of neurons decreased，and the expression of M1 receptor protein was down?regulated.Catalpol could increase the expression of M1 receptor protein significantly(compared with the model group，catalpol low dose group t＝3.983，P＝0.016 4 in the low?dose catalpol group；t＝6.694，P＝0.002 6 in the high?dose catalpol group).The expressions of M1 receptor protein in rats from 4 groups were 100%，64.75%，74.63%，and 85.74% respectively.Conclusion Catalpol could improve the abnormal structure of cerebral cor? tex in AD rats and increase the expression of M1 receptor. |
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