| 赵明理,刘雷,曹建西,等.微 RNA?346对食管癌细胞生物学行为影响的分子机制研究[J].安徽医药,2020,24(2):259-262. |
| 微 RNA?346对食管癌细胞生物学行为影响的分子机制研究 |
| Molecular mechanism of miR?346 on the biological behavior of esophageal cancer cells |
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| DOI：10.3969/j.issn.1009?6469.2020.02.012 |
| 中文关键词: 微 RNAs 食管肿瘤 Yes相关蛋白 1 增殖 凋亡 |
| 英文关键词: MicroRNAs Esophageal neoplasms Yes related protein 1 Proliferation Apoptosis |
| 基金项目:河南省中医药科学研究专项课题( 2019ZY1203)；河南省高等学校重点科目项目( 20B360006) |
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| 中文摘要: |
| 目的观察微 RNA?346(miR?346)对食管癌细胞生物学行为的影响，并探讨其分子机制。方法通过实时荧光定量 PCR(qPCR)检测食管癌组织和细胞株 miR?346的表达量。以表达量最低的细胞为感染对象，分别感染 miR?346慢病毒(实验组)和空载慢病毒(对照组)。 MTT法和集落形成实验分别检测细胞活力和增殖能力，流式细胞术检测凋亡率。 qPCR和蛋白质印迹法检测细胞中 Yes相关蛋白 1(YAP1)及细胞周期蛋白 E(Cyclin E)、细胞凋亡抑制蛋白 1(cIAPl)表达水平。结果食管癌组织和细胞株中 miR?346表达量均明显下降( P＜0.01)TE?1细胞下降最明显。实验组感染 miR?346的细胞活力(实验组 0.93±0.06，对照组1.37±0.06)和增殖能力(对照组 232.3±3740，实验组 82.55±9.66)降低( P＜0.01)，细胞凋亡率升高(实验组 8.26%±0.80%，对照组 0.94%±0.28，P＜0.01)YAP1、Cyclin E和 cIAPl蛋白表达显著下调。结论 miR?346在食管癌组织和细胞中低表达，可明显抑制 TE?1细胞的增殖和诱，导凋亡，其作用机制可能是靶向干扰 YAP1基因的表达。 |
| 英文摘要: |
| Objective To observe the effect of microRNA?346(miR?346)on the biological behavior of esophageal cancer cells and discuss its molecular mechanism.Methods The expression of miR?346 in esophageal cancer tissues and cell lines was detect?ed by real?time quantitative PCR(qPCR).The cells with the lowest expression of miR?346 were infected with miR?346 lentivirus(experimental group)and empty lentivirus(control group).MTT assay and colony formation assay were used to detect the viabilityand proliferation of cells.Flow cytometry was used to detect the apoptotic rate.The expressions of Yes related protein 1(YAP1)，cy? clin E and apoptosis protein 1(cIAPl)were detected by qPCR and Western blot.Results The expression of miR?346 in esopha? geal carcinoma and cell lines was significantly decreased(P＜0.01)，TE?1 cells decreased most significantly(P＜0.01).The cell viability［( 0.93±0.06)vs.(1.37±0.06)］ and proliferation ability［( 232.3±34.40)±(82.55±9.66)］ of cells infected with miR?346 were decreased［( 8.26%±0.80%)vs.(0.94%±0.28%)P＜0.01］the apoptosis rate was increased(P＜0.01).The expression of YAP1，CyclinEandcIAP1proteinwasalsosignificantlyd，ecreased(，P＜0.01).Conclusion miR?346 is low?expressed in esopha? geal cancer tissues and cells，which can significantly inhibit the proliferation and induce apoptosis of TE?1 cells.The mechanismmay be that the expression of YAP1 gene is interfered by miR?346. |
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