| 董松武,张静.急性心肌梗死病人血浆中微小 RNA?21定量检测及其临床意义[J].安徽医药,2020,24(2):337-341. |
| 急性心肌梗死病人血浆中微小 RNA?21定量检测及其临床意义 |
| The quantitative detection and its clinical significance of miR?21 in patients that with acute myocardial infarction |
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| DOI：10.3969/j.issn.1009?6469.2020.02.031 |
| 中文关键词: 心肌梗死 肌钙蛋白 荧光免疫测定 实时聚合酶链反应 微小 RNA?21 实时荧光定量 PCR |
| 英文关键词: Myocardial infarction Troponin Fluoroimmunoassay Real?time polymerase chain reaction microRNA?21 Real?time fluorescent quantitative PCR |
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| 中文摘要: |
| 目的探讨微小 RNA(miRNA)?21表达在急性心肌梗死( AMI)病人血浆中的表达及其临床意义。方法选取 2016年 8月至 2017年 8月亳州市人民医院住院 AMI病人 40例为心肌梗死组，同期该院体检健康者作为健康对照 40例为健康对照组，采用特异的茎环引物逆转录及荧光定量 PCR法检测 miR?21达到设定荧光阈值时的 Ct值，采用 2-△△Ct相对定量法分析 AMI组血浆中 miR?21相对于健康对照组血浆中的表达量；采用受试者工作特征曲线( ROC)评价 miRNA?21、肌钙蛋白在 AMI病人中的诊断效率；采用 Pearson相关性分析 AMI病人血浆中 miR?21表达和肌钙蛋白的相关性。结果 AMI病人血浆中 miR?21相对表达量( 2-△△Ct)为 2.51(0.68～5.00)，心肌梗死组血浆中 miR?21表达明显高于健康对照组，与健康对照组血浆 miR?21的表达水平差异有统计学意义( P＜0.05)；在 AMI病人 miR?21的 AUC为 0.88，肌钙蛋白的 AUC为 0.98；在 AMI病人血浆中 miR?21水平和肌钙蛋白浓度成正相关，相关系数为 0.48(P＜0.05)。结论 AMI病人血浆中 miR?21高表达，可能与心肌梗死发生有关，有望成为心肌梗死诊断的新靶点，为临床诊疗提供新的思路。 |
| 英文摘要: |
| Objective to explore the clinical significance of miR?21 expression in acute myocardial infarction(AMI).Methods using specific stem?loop primers reverse transcription and fluorescence quantitative PCR method to detect the Ct value of miR?21that was reached the threshold setted by fluorescent.using 2-△△Ct relative quantitative method in analysis of 40 cases of AMI pa? tients plasma’s miR?21 expression relatively to the expression in 40 cases of healthy controls.The diagnostic efficiency of miRNA?21 and troponin in AMI patients was evaluated by receiver operating characteristic curve(ROC curve)，and the correlation of miR? 21 expression and troponin in the plasma of AMI patients was analyzed by Pearson correlation.Results the expression level of miR?21was higher in patients with AMI than in healthy control group，the expression multiple(2-△△Ct)is 1.713(0.68～5.00)， and there was statistically significant difference in the two groups(t＝10.20，P＜0.05).In patients with AMI，the AUC of miR?21 was 0.88，and the AUC of troponin was 0.98；In the plasma of AMI patients，the level of miR?21 was positively correlated with the con? centration of troponin，and the correlation coefficient was 0.48(P＜0.05).Conclusion The high expression of miR?21 in patients with AMI may be associated with myocardial infarction occurred，miR?21 plays a role in myocardial infarction process，provides a new clue of clinical diagnosis and treatment. |
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