| 杨娟,郭丽娜,王单单,等.基于网络药理学研究三草降压汤抗糖尿病合并高血压的分子机制[J].安徽医药,2020,24(3):433-437. |
| 基于网络药理学研究三草降压汤抗糖尿病合并高血压的分子机制 |
| The study of molecular mechanism of san cao depressurization decotion against diabetic mellitus combined with hypertension based on network pharmacology |
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| DOI：10.3969/j.issn.1009?6469.2020.03.003 |
| 中文关键词: 糖尿病 /中药疗法 高血压 /中药疗法 槲皮素 山柰酚 肿瘤抑制蛋白质 p53 肿瘤坏死因子类 丝裂原激活蛋白激酶类 三草降压汤 网络药理学 |
| 英文关键词: Diabetes mellitus/drug therapy(TCD) Hypertension/drug therapy(TCD) Quercetin Kaempferol Tumor suppressor proteinp53 Tumornecrosisfactors Mitogen?activatedproteinkinases San cao depressurizationdecotion Networkpharmacology |
| 基金项目:漯河市青年拔尖人才资助项目( 2018QNBJRC01005)；漯河市2018年度市级创新型科技团队， 2018年度漯河市工程技术研究中心(漯科〔 2018〕88号) |
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| 中文摘要: |
| 目的采用网络药理学方法研究三草降压汤抗糖尿病合并高血压的活性成分、潜在作用靶点和信号通路，探讨其抗糖尿病合并高血压的“多成分 -多靶点 -多通路”分子机制。方法基于中药系统药理学数据库和分析平台( TCMSP)和毒性与基因比较数据库( CTD)及中国知网筛选三草降压汤相关的成分及作用靶点，利用 CTD、药物靶标数据库( TTD)、生物信息学和化学信息学数据库( DrugBank)和遗传药理数据库( PharmGKB)检索糖尿病合并高血压相关靶点；采用 Cytoscape 3.7插件获得三草降压汤活性成分和糖尿病合并高血压的共同作用靶点。通过 String在线分析平台获得共同作用靶点的“蛋白质 ?蛋白质”相互作用关系( PPI)网络图，最后通过 DAVID的 GO富集分析和 KEGG通路富集分析研究共同作用靶点。结果筛选出三草降压汤的 22个活性成分， 72个关键靶点， 21条显著信号通路。三草降压汤抗糖尿病合并高血压的主要活性成分是槲皮素、山柰酚、木樨草素等，潜在作用靶点和信号通路分别为肿瘤抑制蛋白( TP53)、肿瘤坏死因子( TNF)、白细胞介素 ?6(IL?6)等靶点和 Toll样受体信号通路、丝裂原活化蛋白激酶( MAPK)信号通路、脂肪因子信号通路等，涉及细胞增殖、凋亡、炎症反应等生物学过程。结论三草降压汤抗糖尿病合并高血压的主要分子机制是固醇类、黄酮类和萜类化合物通过影响丝裂原活化蛋白激酶信号通路、脂肪因子信号通路中的相关靶点如 TP53、TNF、IL?6等发挥药效。 |
| 英文摘要: |
| Objective To study the active components，potential targets，signaling pathways of san cao depressurization decotion against diabetic mellitus combined with hypertension based on network pharmacology，and to explore the molecular mechanism of "multi?component，multi?target and multi?pathway" against diabetes combined with hypertension.Methods The traditional Chinese medicine systems pharmacology(TCMSP)，comparative toxicogenomics database(CTD)and CNKI databases were used for screen? ing the components and potential targets of san cao depressurization decotion，and the targets of diabetic mellitus combined with hypertension were obtained from the CTD，Statistics of Therapeutic Target Database(TTD)，DrugBank and the Pharmacogenomics Knowledgebase(PharmGKB)databases.The same targets of san cao depressurization decotion and diabetic mellitus combined withhypertension were screened through plug in Cytoscape 3.7.Then the Protein?Protein Interaction(PPI)networks of the same tar? gets of them were constructed through String database respectively，and then analysed through GO and KEGG enrichment analysis. Results A total of 22 active components，72 targets and 21 remarkable pathways of san cao depressurization decotion were obtained. The main active components of san cao depressurization decotion against diabetic mellitus combined with hypertension were querce? tin，kaempferol，luteolin，and so on.The potential targets were TP53，TNF，IL?6，and so on，and the signaling pathways were T cell re? cepter signaling pathway，Toll recepter signaling pathway，adipocytokine signaling pathway and MAPK signaling pathway，and so on， which were mainly.involved in cell proliferation，apoptosis and inflammation action.Conclusions The main molecular mechanism of san cao depressurization decotion against diabetes complicated with hypertension is that sterols，flavonoids and terpenes exert their ef?fects by influencing related targets in MAPK signaling pathway and adipokine signaling pathway，such as TP53，TNF and IL?6.T |
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