| 宗桂娟,尹海兵,李春笋,等.K?RAS蛋白表达及基因状态在胃癌组织中的临床意义[J].安徽医药,2020,24(4):705-708. |
| K?RAS蛋白表达及基因状态在胃癌组织中的临床意义 |
| Clinical significance of K?RAS protein expression and gene status in gastric carcinoma |
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| DOI：10.3969/j.issn.1009?6469.2020.04.017 |
| 中文关键词: 胃肿瘤 基因， ras 突变 免疫组织化学 荧光免疫测定 K?RAS 临床病理特征 |
| 英文关键词: Stomach neoplasms Genes,ras Mutation Immunohistochemistry Fluoroimmunoassay K?RAS Clinico? pathological characteristics |
| 基金项目:南通市科技项目( HS2015003) |
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| 中文摘要: |
| 目的分析胃癌病人的 K?RAS蛋白表达及基因状态与临床病理特征的关系，为胃癌病人的个性化治疗提供指导。方法选取 2016年 1月至 2017年 12月南通市肿瘤医院 314例胃癌病人，分别采用免疫组织化学法及荧光定量 PCR的方法分析 K?RAS蛋白表达水平和基因突变状态。结果 314例胃癌组织中 K?RAS蛋白表达与胃癌病人的年龄( P＝0.648 5)、性别( P＝0.585 8)、肿瘤大小( P＝0.761 1)、脉管侵犯( P＝0.552 4)、淋巴结转移均无明显关系( P＝0.506 4)，与组织学分级密切相关(P＝0.019 5)； K?RAS基因突变类型包括 G12D、G13D、G12A、G12S、G13S；不同性别( P＝0.456 6)、年龄( P＝0.524 2)、组织分化之间(P＝0.747 4)的 K?RAS突变率差异无统计学意义； K?RAS基因突变率与蛋白表达检出率差异有统计学意义( P＝0.037 7)。结论 K?RAS的蛋白表达程度与肿瘤的分化程度有关， K?RAS基因在不同性别、年龄、组织分化之间的突变率差异无统计学意义，且 K?RAS基因突变率与蛋白表达不一致。 |
| 英文摘要: |
| Objective To analyze the relationship between K?RAS protein expression and gene status and clinicopathological fea?tures in patients with gastric carcinoma，and to provide guidance for personalized treatment of gastric carcinoma patients.Methods A total of 314 patients with gastric cancer in Nantong Cancer Hospital from January 2016 to December 2017 were selected.The ex?pression level of K?RAS protein and the state of gene mutation were analyzed by immunohistochemical method and fluorescentquantitative PCR.Results The expression of K?RAS protein in 314 cases of gastric carcinoma had no significant relationship with age(P＝0.648 5)，sex(P＝0.585 8)，tumor size(P＝0.761 1)，vascular invasion(P＝0.552 4)and lymph node metastasis(P＝0.506 4)，which was closely related to histological grade(P＝0.019 5).K?RAS gene mutation types include G12D，G13D，G12A， G12C，G13S；K?RAS mutation rates were not significantly different between genders(P＝0.456 6)age(P＝0.524 2)，and tissue tistically different(P＝0.037 differentiation(P＝0.7474).K?RASgenemutationrateandproteinexpressiondetectionrateweresta，7).Conclusion The protein expression of K?RAS is related to the degree of tumor differentiation.There is no significant differencein the mutation rate between K?RAS gene in different gender，age and tissue differentiation，and the K?RAS gene mutation rate is inconsistent with protein expression. |
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