| 李华,彭音,陈芳,等.白及二氢菲类化合物对临床常见病原菌作用的研究[J].安徽医药,2020,24(4):800-804. |
| 白及二氢菲类化合物对临床常见病原菌作用的研究 |
| Effect of dihydrophenanthropyrans of bletilla striata on common clinical pathogenic bacteria |
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| DOI：10.3969/j.issn.1009?6469.2020.04.041 |
| 中文关键词: 白及 二氢菲类化合物 纸片扩散抗菌试验 血清杀菌试验 抑菌作用 逆转耐药性作用 |
| 英文关键词: Bletilla striata Dihydrophenanthropyrans Disk diffusion antimicrobial tests Serum bactericidal test Bacterio? stastic activity Reversal of drug resistance |
| 基金项目:国家自然科学基金项目(31560079)；绵阳市卫计委补助科研项目( 201604)；绵阳市卫计委科研鼓励项目( 201629)；江油市科技支撑项目( 201627) |
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| 中文摘要: |
| 目的探讨白及二氢菲类化合物对临床常见病原菌的作用，为白及药理活性成分的临床应用提供实验依据。方法实验以琼脂稀释法测定不同白及组织的二氢菲类化合物(Dihydrophenanthropyrans，Dihs)对金黄色葡萄球菌等 6种常见病原菌及标准菌株的体外最低抑菌浓度(MIC)常见病原菌均采自同一科室的临床菌株，实验时间从 2017年5月至 2018年5月。接着将菌株接种到 Dihs浓度为 MIC的培养基及对，照培养基上，共培养 48 h进行抗生素药敏试验，然后测量抑菌圈直径，结合菌斑图，判断 Dihs对临床菌株及抗生素的药理作用。结果白及 Dihs具有抑制临床病原菌生长的作用，且不同材料的 Dihs对菌株的 MIC值影响不尽相同，其中悬浮细胞组织中的 Dihs对金黄色葡萄球菌的 MIC值相对于其他白及组织最小，为(0.58±0.23)mg/mL。同时， Dihs有增强病原菌对抗生素敏感性的作用，但是对某些抗生素 Dihs也有降低病原菌对抗生素的敏感性的作用，其中， Dihs增强白假丝酵母菌对抗生素敏感性作用较大，抑菌圈直径平均增加了(4.4±0.3)mm。进一步从实验结果还可以发现，白及 Dihs具有逆转某些病原菌耐药性的作用。结论白及二氢菲类化合物对临床常见病原菌具有抑制作用和逆转耐药性的作用。本研究为白及的药理学研究提供了基础数据的参考，也为江油地区白及的抑菌等活性成分的利用和临床新型抗生素的研究提供理论依据。 |
| 英文摘要: |
| Objective To investigate the effects of dihydrophenanthropyrans of Bletilla striata on common pathogenic bacteria，and to provide experimental evidence for the clinical application of Bletill astriata’s pharmacological active ingredients.Methods In vi? tro minimum inhibitory concentration(MIC)of dihydrophenanthropyrans(Dihs)from different tissues of Bletilla striata against 6common pathogens including staphylococcus aureus and standard strains was determined by AGAR dilution method.The commonpathogens were all clinical strains from the same department，and the experimental time was from May 2017 to May 2018.Then thestrain was inoculated into the medium with MIC of Dihs concentration and the control medium，and incubated for 48 h for antibiot? ic susceptibility test.Then，the diameter of the inhibition zone was measured and the pharmacological effects of Dihs on clinicalstrains and antibiotics were determined according to the plaque diagram.Results The results showed that Dihs of Bletilla striata could inhibit the growth of clinical pathogenic bacteria，and the MIC values of Dihs in different tissues had different effects on bac? terial strains.Compared with other tissues of Bletilla striata，the MIC values of Dihs in suspended cell tissue to Staphylococcus aure? us was the lowest，which was 0.58±0.23 mg/mL.Meanwhile，Dihs could enhance the sensitivity of pathogenic bacteria to antibiotics， but Dihs could also reduce the sensitivity of pathogenic bacteria to some antibiotics.Among them，Dihs enhanced the sensitivity of Candida albicans to antibiotics，and the diameter of inhibition circle increased by 4.4±0.3 mm on average.Furthermore，it was found that Dihs of Bletilla striata could reverse the drug resistance of some pathogens.Conclusions Dihs of Bletilla striata could inhibit the growth of clinical pathogenic bacteria，and reverse the drug resistance of some bacteria.This study provides basic data for thepharmacological research of Bletilla striata and theoretical basis for the utilization of antibacterial and other active ingredients ofBletilla striata in Jiangyou area and the research on new clinical antibiotics. |
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