| 郭浩,周淑妮,冉瑞智.苯丁酸钠对乳腺癌细胞增殖和凋亡的影响[J].安徽医药,2020,24(6):1090-1093. |
| 苯丁酸钠对乳腺癌细胞增殖和凋亡的影响 |
| Effects of sodium phenylbutyrate on proliferation and apoptosis of human breast cancer cell |
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| DOI：10.3969/j.issn.1009?6469.2020.06.007 |
| 中文关键词: 乳腺肿瘤 苯丁酸钠 抗肿瘤联合化疗方案 细胞凋亡 BRCA1蛋白质 |
| 英文关键词: Breast neoplasms Sodium phenylbutyrate Antineoplastic combined chemotherapy protocols Apoptosis BRCA1 protein |
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| 中文摘要: |
| 目的观察分析苯丁酸钠( Sodium phenylbutyrate，NaPB)对乳腺癌 MDA?MB?231细胞增殖和凋亡的影响，初步探究其可能的作用机制。方法以不同剂量 NaPB(0 mmol/L、2 mmol/L和 4 mmol/L)处理乳腺癌 MDA?MB?231细胞株并进行实验分组:对照组， 2 mmol/L NaPB组 4 mmol/L NaPB组。采用四甲基偶氮唑盐微量酶反应比色法( MTT法)检测各组乳腺癌细胞的增殖情况，使用流式细胞仪检测细胞凋亡，应用蛋白质印迹法(Western Blot)检测凋亡相关蛋白 B细胞淋巴瘤 /白血病 ?2(Bcl?2)、Bcl?2相关 X蛋白( Bax)和乳腺癌易感基因 1(BRCA1)蛋白表达的水平。结果 NaPB能显著抑制乳腺癌 MDA?MB?231细胞的生长增殖，并可诱导乳腺癌细胞凋亡，对照组、 2 mmol/L NaPB组和 4 mmol/L NaPB组乳腺癌细胞的凋亡率分别为( 4.25±0.89)%、(14.11±2.98)%和( 32.73±1.89)%，两两比较均差异有统计学意义( P＜0.05)NaPB可诱导凋亡相关蛋白 Bcl?2表达水平下降，而 Bax表达水平升高， NaPB的抗肿瘤作用在高剂量组中表现得更明显。与对相比 NaPB可显著下调 BRCA1蛋白的表达，差异照组，有统计学意义(P＜0.05)。结论 NaPB可抑制乳腺癌细胞增殖和诱导细胞凋亡，其作用机制可能与 NaPB下调 BRCA1表达有关。 |
| 英文摘要: |
| Objective To study the effects of sodium phenylbutyrate(NaPB)on the proliferation and apoptosis of human breast can? cer cell Line MDA?MB?231，and to explore the probable mechanism.Methods MDA?MB?231 cells were treated with different con? centration of NaPB(0 mmol/L、2 mmol/L and 4 mmol/L)，and assigned into control group，2 mmol/L NaPB group and 4 mmol/L NaPB group.Tetramethylazoazole salt microenzyme reaction colorimetric method(MTT)assay was performed to detect the prolifera?tion of MDA?MB?231 cells.The cell apoptosis was tested using flow cytometry.Western Blot assay was carried out to examine theapoptosis?related proteins Bcl?2，Bax and breast cancer gene 1(BRCA1).Results NaPB significantly inhibited the growth and pro?liferation of breast cancer MDA?MB?231 cells and induced apoptosis of breast cancer cells，the average apoptosis rate in control group，2 mmol/L and 4 mmol/L NaPB group was(4.25±0.89)%，(14.11±2.98)% and(32.73±1.89)%，respectively，and the differ? ence between the two pairs was statistically significant(P＜0.05).NaPB decreased the expression level of apoptosis?related protein Bcl?2 and increased the expression level of Bax，and the anti?tumor effect of NaPB was more obvious in the high?dose group.Com? pared with the control group，NaPB significantly reduced the expression of BRCA1 protein，and the difference was statistically sig? nificant(P＜0.05).Conclusion NaPB can inhibit breast cancer cell proliferation and induce apoptosis，and its mechanisms may be related to down?regulation of BRCA1expression. |
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