| 张斌强,陈龙,冉凤英,等.乌头碱对 H9c2细胞分化的影响[J].安徽医药,2020,24(7):1287-1290. |
| 乌头碱对 H9c2细胞分化的影响 |
| Effects of aconitine on H9c2 cell differentiation |
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| DOI：10.3969/j.issn.1009?6469.2020.07.004 |
| 中文关键词: 乌头碱 肌细胞，心脏 细胞分化 H9c2 肌钙蛋白 I α肌动蛋白 |
| 英文关键词: Aconitine Myocytes,cardiac Cell differentiation H9c2 Troponin I α?actinin.6,(5,Aconitine, |
| 基金项目:湖北省教育厅科学研究计划指导性项目(B2018435、B2016140)；湖北省卫生健康科研基金资助( WJ2019M054)；十堰市科学技术研究与开发项目(17Y49、17Y53、17Y54) |
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| 中文摘要: |
| 目的观察乌头碱对 H9c2细胞分化的影响。方法用不同浓度的乌头碱作用于大鼠 H9c2心肌细胞 5d后噻唑蓝比色法( MTT)检测细胞的增殖率。选取 50 μM/L的乌头碱作用于全反式维甲酸( RA)诱导分化体系下的大鼠 H9c2心肌细胞。光学显微镜观察细胞形态变化，荧光定量 PCR(qPCR)检测 actc1、myl1、myl2、tnnt2、nppb的 mRNA表达，免疫荧光细胞化学染色检测 α肌动蛋白( α?actinin)分布情况。蛋白质印迹法( Western Blot)检测 α?actinin、心肌肌钙蛋白(I cTnI)蛋白的表达。采用单因素方差分析对比组间差异。结果乌头碱能够呈剂量依赖性的抑制 H9c2心肌细胞增殖( P＜0.05)。较分化模型组，乌头碱处理组中分化细胞形态减少， actc1［(2.30±0.17)比( 0.55±0.03)，F＝62.17、P＝0.000］，myl1［(2.57±0.15)比( 1.22±0.13)，F＝63.15、 P＝0.000］myl2［( 18.64±0.53)(10.02±1.10)F＝206.65、P＝0.000］tnnt2［( 5.53±0.23)(2.23±0.10)F＝216.89、P＝0.000］的 mRNA表达降，低( P＜0.01)差异有统计学意义，c，TnI及 α?actinin的蛋白表，达降低。结论乌头碱能够抑制，H9c2心肌细胞分化。 |
| 英文摘要: |
| Objective To observe the influence of aconitine on the H9c2 cells differentiation.Methods The proliferation rate of rats’H9c2 cardiomyocytes was detected by thiazolyl blue colorimetric method(MTT)treated with different concentrations of acon? itine after 5days.50μM/L aconitine was selected to act on H9c2 under the induced differentiation system of all?trans Retinoic Acid(RA).The morphological changes of cells were observed by optical microscopy.The mRNA expression of actc1，myl1，myl2，tnnt2 and nppb was detected by fluorescence quantitative PCR(qPCR)，and distribution of cardiac α?actinin was detected by immunofluo?rescence cytochemical staining.The expressions of cardiac troponin I(cTnI)and α?actinin protein were detected by Western?Blot.Statistical analysis was performed by One?way analysis of variance to compare differences between groups.Results Aconitine can inhibit the proliferation of H9c2 in a concentration?dependent manner.Aconitine can inhibit the mRNA expression ofactc1［( 2.30±0.17)vs.(0.55±0.03)F＝62.17、P＝0.000］myl1［( 2.57±0.15)vs.(1.22±0.13)，F＝63.15、P＝0.000］，myl2［( 18.64±0.53)vs.(10.02±1.10)，F＝2065、P＝0.000］tnnt2［.53±0.23)vs.(2.23±0.10)，F＝216.89、P＝0.000］and the proteins expression of cT? nI and α?actinin.Conclusion can inhibit differentiation of H9c2 cardiomyocytes. |
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