| 董晤讯,马勇,郭杨,等.富血小板血浆对兔腰背根神经节细胞凋亡的影响[J].安徽医药,2020,24(7):1374-1377. |
| 富血小板血浆对兔腰背根神经节细胞凋亡的影响 |
| Effect of platelet?rich plasma on apoptosis of lumbar dorsal root ganglion cells in rabbits |
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| DOI：10.3969/j.issn.1009?6469.2020.07.026 |
| 中文关键词: 富血小板血浆 神经节，脊 椎间盘移位 细胞凋亡 组织学形态 兔 |
| 英文关键词: Platelet?rich plasma Ganglia,spinal Intervertebral disc displacement Apoptosis Histological morphology Rabbits |
| 基金项目:江苏省金坛市科技计划社会发展项目(JT2013070、JT201461) |
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| 中文摘要: |
| 目的观察富血小板血浆对兔背根神经结扎加自体髓核移植所致背根神经节组织学形态及神经元凋亡的作用，并初步探讨其作用机制。方法 2017年 10月至 2018年 2月，将 48只新西兰兔按随机数字表法分为治疗组、对照组和假手术组，造模后治疗组给予相同剂量的自体富血小板血浆，对照组组给予等体积自体脂肪组织，假手术组只暴露背根神经节。取相应背根神经节标本，行石蜡包埋切片后进行 HE染色、 TUNEL法染色，镜下观察并计算阳性细胞数，所得数据用 SPSS 19.0统计软件进行分析。结果各组大鼠 96 h背根神经节 HE染色可见:对照组神经元皱缩，排列欠规则，细胞间隙宽，尼氏小体变少，细胞核缩小；治疗组细胞排列规则，细胞间隙无明显增宽；假手术组无明显病理变化。各个时间点，治疗组 24 h(15.29±0.98)个、 48 h(12.32±0.85)个、 72 h(14.55±0.79)个、 96 h(15.29±0.98)个的 TUNEL法阳性细胞数明显低于对照组的 24 h(21.93±2.44)个、 48 h(24.34±3.82)个、 72 h(23.83±3.30)个、 96 h(27.45±1.44)个，但高于假手术组 24 h(5.36±1.05)个、 48 h(5.27±1.22)个、 72 h(4.20± 0.46)个、 96 h(4.98±0.72)个( P＜0.05)。结论兔腰背根神经节结扎加自体髓核移植可造成神经元凋亡；富血小板血浆可以抑制细胞凋亡，对神经元具有保护作用。 |
| 英文摘要: |
| Objective To observe the effect of platelet?rich plasma on the histological morphology and neuronal apoptosis of dorsalroot ganglion in rabbits with dorsal root nerve ligation and autologous nucleus pulposus transplantation，and to explore the mecha? nism of action.Methods From October 2017 to February 2018，forty?eight New Zealand rabbits were divided into treatment group， control group and sham operation group according to the random number table method.After the model was established，the same dose of autologous platelet?rich plasma was administered to the treatment group，and the control group was given an equal volume of autologous adipose tissue，the sham?operated group only exposed the dorsal root ganglion.The corresponding dorsal root ganglion specimens were taken，paraffin?embedded and sliced for HE and TUNEL staining.The number of positive cells was observed undermicroscope and the data was analyzed with SPSS 19.0 statistical software.Results 1.HE staining of dorsal root ganglion at 96h in each group showed that neurons in the control group shrank，the arrangement was irregular，the cell gap was wide，the Nissl body was reduced，and the nucleus was reduced.The cells in the treatment group had regular arrangement and cell gaps.No significant widening；no significant pathological changes in the sham group.2.At various time points，the number of TUNEL positive cells in treatment group was significantly lower than that in the control group at 24h(15.29±0.98)，48h(12.32±0.85)，72h(14.55±0.79)， 96h(15.29±0.98)at 24h(21.93±2.44)48h(24.34±3.82)72h(23.83±3.30)，96h(27.45±1.44)，but higher than sham operation group 24h(5.36±1.05)，48h(5.27±1.22)，，72h(4.20±0.46)，，96h(4.98±0.72)( P＜0.05).Conclusion Rabbit lumbar dorsal root ganglion ligation plus autologous nucleus pulposus transplantation can cause neuronal apoptosis，platelet?rich plasma can inhibit cell apoptosis and protect neurons. |
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