| 邓春颖,李海滨,毛文静,等.丁苯酞对阿尔茨海默病模型大鼠海马CA1区Wnt3a及β-连环蛋白表达的影响[J].安徽医药,2020,24(9):1701-1704. |
| 丁苯酞对阿尔茨海默病模型大鼠海马CA1区Wnt3a及β-连环蛋白表达的影响 |
| The effects of dl-3-n-butylphthalidle on the expression of Wnt3a and β-catenin in CA1 region of hippocampus of rats with Alzheimer disease |
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| DOI：10.3969/j.issn.1009-6469.2020.09.002 |
| 中文关键词: 阿尔茨海默病 海马 丁苯酞 Wnt3a蛋白 β-连环蛋白 大鼠， Sprague-Dawley |
| 英文关键词: Alzheimer disease Hippocampus Dl-3-n-butylphthalidle Wnt3a protein β-Catenin Rats,Sprague-Dawley |
| 基金项目:河北省重点研发计划自筹项目( 172777151)；华北理工大学科学研究基金项目( Z201734) |
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| 中文摘要: |
| 目的观察丁苯酞( dl-3-n-butylphthalidle)软胶囊对阿尔茨海默病( Alzheimer disease，AD)模型大鼠海马 CA1区 Wnt3a蛋白及 β-连环蛋白( β-catenin)表达的影响，探寻丁苯酞发挥脑保护作用的可能机制。方法将 72只雄性 SD大鼠按随机数字表法分为假手术组( Sham组)、 AD模型组( AD组)、丁苯酞治疗组(丁苯酞组)。向双侧海马区注射 β-淀粉样蛋白 1-42(beta-amy- loid people 1-42，Aβ1-42)制作 AD模型， Sham组注射 5 μL 0.9%氯化钠溶液。造模成功后 4周，丁苯酞组给予丁苯酞与食用油混合后制成的丁苯酞混合溶液灌胃； Sham组和 AD组采用同等剂量的食用油进行灌胃。采用免疫组织化学法和蛋白质印迹法分别于给药后 1、2、4和 8周检测海马 CA1区 Wnt3a及 β-catenin表达情况。结果蛋白质印迹法表明与 Sham组给药后 1、2、4和 8周［(3 845.25±52.35)(4 385.06±108.85)(9 392.19±81.07)(6 833.15±76.03)］相比， AD组各时间点 Wnt3a蛋白表达量增多［(5595.54±94.60)(7223.89，±86.73)(13 671.83，±312.38)(11 641.，04±203.65)均P＜0.01］；各时间点 β-catenin蛋白表达量差异无统计学意义(P＞0.05)，。与 AD组［(4132，.90±79.63)(4 758.7，4±155.14)(5 188.92，±111.82)，(5 912.07±83.26)］相比，丁苯酞组各时间点 Wnt3a蛋白［(6261.26±206.51)(8427.83±293.61)，(16469.74±204.5，5)(13438.21±12399.19)］、β-catenin蛋白表达量明显增多［(5 569.53±96.52)(7 127.78±69.64)，(11 454.94±396.4，3)(9 937.91±157.87)，均P＜0.01］。免疫组织化学法和蛋白质印迹法检测结果相同。结论丁苯，酞可通过上调W，nt3a及β-catenin蛋白，的表达，发挥对 AD模，型大鼠的脑保护作用。 |
| 英文摘要: |
| Objetive Assessed the effects of Dl-3-n-butylphthalidle on the expression of Wnt3a and β-catenin in CA1 region of hip-pocampus of rats with Alzheimer disease，explored the mechanism of brain protection by butylphthalide.Methods 72 SD rats were randomly divided into the sham-operation group，the Alzheimer disease model group(AD group)and the NBP treated group(NBP group).The Alzheimer disease rat model was prepared by injecting beta-amyloid people 1-42 into bilateral hippocampus.Four weeksafter the model was successfully established，the NBP group was given the mixture solution of NBP and edible oil by gavage，while the Sham group and AD group were given the same dose of edible oil by gavage.The expressions of Wnt3a and β-catenin in the hip-pocampal CA1 region were detected at 1，2，4 and 8 weeks after administration by immunohistochemistry and Western blotting. Results Western blotting showed that compared with the results of 1，2，4，and 8 weeks after taking medicines in the sham-opera- tion group which were［( 3 845.25±52.35)，(4 385.06±108.85)，(9 392.19±81.07)，(6 833.15±76.03)］，the expression of Wnt3a protein increased in AD group at each time points［( 5 595.54±94.60)，(7 223.89±86.73)(13 671.83±312.38)，(11 641.04± 203.65)］，thedatadifferenceswerestatisticallysignificant(P＜0.01).Thedatadifferenceofthe，expression of β-catenin in the two groups was not statistically significant(P＞0.05).Compared with the AD group［(4 132.90±79.63)，(4 758.74±155.14)，(5 188.92± 111.82)(5 912.07±83.26)］，the expression of Wnt3a protein［( 6 261.26±206.51)(8 427.83±293.61)(16 469.74±204.55)，(13 438.21，±12 399.19)］and β-catenin［(5 569.53±96.52)(7 127.78±69.64)(11 454.，94±396.43)(9 937.9，1±157.87)］both in- creased in NBP group at each time points，thedatadifferences，werestatisticallysi，gnificant(all P＜0.01).，The results of immunohis- tochemistry and western blotting were the same.Conclusion NBP can protect the brain of AD model rats by up-regulating the ex-pression of Wnt3a and-catenin protein. |
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