| 丁波,李智敏,黄建玲.黄芪多糖对高氧致新生大鼠支气管肺发育不良 mir?34a/sirt1轴的影响[J].安徽医药,2020,24(10):1927-1932. |
| 黄芪多糖对高氧致新生大鼠支气管肺发育不良 mir?34a/sirt1轴的影响 |
| Effect of astragalus polysaccharide on mir?34a/sirt1 axis in hyperoxia?induced bronchopulmonary dysplasia in neonatal rats |
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| DOI：10.3969/j.issn.1009?6469.2020.10.004 |
| 中文关键词: 支气管肺发育不良 黄芪多糖 高氧 超氧化物歧化酶 微小 RNA?34a 沉默信息调节因子 1 |
| 英文关键词: Bronchopulmonary dysplasia Astragalus polysaccharide Hyperoxia Superoxide dismutase MicroRNA?34a Silence information regulator 1 |
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| 中文摘要: |
| 目的探讨黄芪多糖(APS)对高氧诱导支气管肺发育不良(BPD)新生大鼠微小 RNA?34a(mir?34a)/沉默信息调节因子 1(sirt1)轴的影响。方法将 2018年 1月至 2019年 1月参加实验的 64只大鼠按照随机数字表法分为空白对照组(NC组)、 APS对照组(APS组)、高氧诱导 BPD模型组(BPD组)、 APS治疗 BPD组(BPD+APS组)每组 16只。 NC组、 APS组暴露在空气中； BPD组、 BPD+APS组大鼠暴露在氧浓度(70±5)%环境中。 24 h后 APS组和 BPD+APS组，腹腔注射 100 mg·kg-1·d-1 APS，NC组和 BPD组腹腔注射同体积生理盐水，每天 1次直至大鼠处死。建模 10、17 d，称量大鼠体质量变化；苏木素 ?伊红(HE)染色观察肺组织形态学变化；酶联免疫吸附试验法(ELISA)检测肺组织中白介素 ?6(IL?6)、白介素 ?10(IL?10)、肿瘤坏死因子 ?α(TNF?α)、丙二醛(MDA)水平及超氧化物歧化酶(SOD)活性变化；实时荧光定量 PCR(qRT?PCR)检测肺组织中 mir?34a水平变化；免疫组化、蛋白质印迹法(Western blot)检测肺组织中 sirt1水平变化。结果 NC组与 APS组建模 10、17 d体质量，肺组织形态， IL?6、 IL?10、TNF?α、MDA、mir?34a、sirt1水平， SOD活性均无明显变化(P＞0.05)。与 NC组、 APS组相比， BPD组建模 10、17 d体质量下降，肺组织结构不完整、肺泡数量减少、体积增大、结构简单化， IL?6、TNF?α、MDA、mir?34a表达升高［(211.32±47.68)比 |
| 英文摘要: |
| Objective To investigate the effects of Astragalus Polysaccharide(APS)on microRNA?34a(mir?34a)/silent information regulator?1(sirt1)axis in neonatal rats with hyperoxia?induced bronchopulmonary dysplasia(BPD).Methods Sixty?four rats from January 2018 to January 2019 were selected and randomly assigned into air control group(NC group)， APS control group(APS group)，hyperoxia?induced BPD model group(BPD group)，and APS treatment BPD group(BPD + APS group)according to the ran? dom number table，with 16 rats in each group.NC group and APS group were exposed to air，rats in BPD group and BPD+APS group were exposed to oxygen concentration(70±5)%.After 24 hours，APS group and BPD+APS group were intraperitoneally inject? ed with 100 mg·kg?1·d?1 APS，while NC group and BPD group were intraperitoneally injected with the same volume of saline，once a day until the rats were executed.After 10 and 17 days of modeling，the weight of rats was weighed，lung histomorphological chang? es were observed by hematoxylin?eosin(HE)staining；the levels of interleukin?6(IL?6)interleukin?10(IL?10)，tumor necrosis factor? α(TNF?α)，malondialdehyde(MDA)andtheactivityofsuperoxidedismutase(SOD)inlu，ng tissues were detected by enzyme?linked immunosorbent assay(ELISA)； real?time fluorescence quantitative PCR(qRT?PCR)was used to detect the level of miR?34a in lung tissue；the levels of sirt1 in lung tissues were detected by immunohistochemistry and Western blot.Results At 10th and 17th days， there was no significant change in the weight，lung tissue morphology，IL?6，IL?10，TNF?α，MDA，miR?34a and sirt1 levels，SOD ac? tivity of NC group and APS group(P＞0.05).Compared with NC group and APS group，at 10th and 17th days，the weight lost in BPD group，the lung tissue structure was incomplete，the number of alveoli decreased，the volume increased，the structure was sim?plified，the expressions of IL?6［(211.32±47.68)ng/L vs.(218.66±48.04)ng/L］，TNF?α［(496.52±81.91)ng/L vs.(537.88±64.06)ng/ L］，MDA［(4.17±1.05)μmol/g vs.(4.32±0.95)μmol/g］and miR?34a［(2.38±0.47)vs.(2.28±0.55)］increased，the expressions of IL? 10［(3.71±0.67)ng/L vs.(3.42±0.53)ng/L］and sirt1［(0.11±0.06)vs.(0.15±0.05)］and SOD activity［(11.64±1.94)U/mg vs.(8.96±1.95)U/mg］decreased(P＜0.05).Compared with BPD group，at 10th days of modeling，the alveoli of lung tissue of BPD + APS group developed well，the size of alveoli was uniform，and the structure of alveoli gradually recovered，the expressions of IL?6［(159.66±39.17)ng/L］，TNF?α［(357.77±65.31)ng/L］and miR?34a［(1.62±0.38)］decreased，the expressions of IL?10［(6.67±1.17) ng/L］and sirt1［(0.94±0.14)］ increased，at 17th days of modeling，the weight increased，the alveolar development of lung tissue was better，the size was uniform，and the structure was gradually close to that of NC group and APS group，the expressions of IL?6［(152.22±31.08)ng/L］，TNF?α［(317.66±73.22)ng/L］，MDA［(2.86±0.71)μmol/g］and miR?34a［(1.49±0.29)］decreased，and the expressions of IL?10［(8.17±1.2)ng/L］，sirt1［(1.02±0.18)］，and SOD activity［(16.95±3.4)］ increased(P＜0.05).Conclusion APS may protect newborn rats with BPD by regulating mir?34a/sirt1 axis to inhibit inflammation and oxidative stress. |
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