| 詹莉琼,卢晏民,崔杰.不同临床病理特征非小细胞肺癌中 miR?1269的表达及其对细胞周期的靶向调控作用[J].安徽医药,2020,24(11):2248-2251. |
| 不同临床病理特征非小细胞肺癌中 miR?1269的表达及其对细胞周期的靶向调控作用 |
| Expression of miR?1269 in non?small cell lung cancer with different clinical pathological characteristics and its targeted regulation on cell cycle |
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| DOI：10.3969/j.issn.1009?6469.2020.11.033 |
| 中文关键词: 癌，非小细胞肺 细胞周期蛋白 D1 细胞周期蛋白质依赖激酶类 miR?1269 病理特征 |
| 英文关键词: Carcinoma,non?small?cell lung Cyclin D1 Cyclin?dependent kinases miR?1269 Pathological features |
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| 中文摘要: |
| 目的研究不同临床病理特征非小细胞肺癌(NSCLC)中 miR?1269的表达及其对细胞周期的靶向调控作用。方法收集 2015年 3月至 2018年 12月商丘市第一人民医院手术切除的 NSCLC病灶和对应的癌旁病灶 58例，检测 miR?1269及细胞周期蛋白的表达量；培养肺癌 A549细胞株，转染 miR?1269的模拟物和抑制物后检测细胞周期蛋白的表达量。结果 NSCLC病灶中 miR?1269的表达量明显高于癌旁病灶(P＜0.05)且低未分化、有淋巴结转移、有脉管浸润、有胸膜侵犯的 NSCLC病灶中 miR?1269的表达量明显高于高中分化、无淋巴结转移、无脉管浸润、无胸膜侵犯的 NSCLC病灶(P＜0.05)； NSCLC病灶中 Cy? clinD1、CDK4、CDK6的表达量明显高于癌旁病灶且与 miR?1269呈正相关， p21Cip1、p27Kip1的表达量明显低于癌旁病灶且与 miR? 1269呈负相关(P＜0.05)。 miR1269模拟物组 A549细胞中 CyclinD1、CDK4、CDK6的表达量明显高于空白对照组、 NC模拟物组， p21Cip1、p27Kip1的表达量明显低于空白对照组、 NC模拟物组(P＜0.05)； miR?1269抑制物组 A549细胞中 CyclinD1、CDK4、 CDK6的表达量明显低于空白对照组、 NC抑制物组， p21Cip1、p27Kip1的表达量明显高于空白对照组、 NC抑制物组(P＜0.05)。结论 miR?1269的高表达与 NSCLC病理特征的变化有关且 miR?1269能够靶向调节细胞周期蛋白的表达。 |
| 英文摘要: |
| Objective To investigate the expression of miR?1269 in non?small cell lung cancer(NSCLC)with different clinical pathological characteristics and its targeted regulation on cell cycle.Methods NSCLC lesions and corresponding pericancerous le?sions were collected from March 2015 to December 2018 in Shangqiu First People’s Hospital，and the expression of miR?1269 and cell cycle protein was detected.The A549 cell line of lung cancer was cultured，and the expression of cell cycle protein was detect? ed after transfection of mimic and inhibitor of miR?1269.Results The expression of miR?1269 in NSCLC lesions was significantly higher than that in adjacent lesions(P＜0.05)，and the expression of miR?1269 in NSCLC lesions with low?undifferentiated，lymphnode metastasis，vascular invasion and pleural invasion were significantly higher than that in NSCLC lesions with high?middle differ? entiation，no lymph node metastasis，no vascular invasion and no pleural invasion(P＜0.05).The expression of CyncliD1，CDK4， CDK6 in NSCLC lesions were significantly higher than those in adjacent lesions and positively correlated with miR?1269，the ex? pression of p21Cip1 and p27Kip1 were significantly lower than those in adjacent lesions and negatively correlated with miR?1269(P＜0.05).The expression of CyncliD1，CDK4，CDK6 in A549 cells of miR?1269 mimic group were significantly higher than those in blank control group and NC mimic group，the expression of p21Cip1 and p27Kip1 were significantly lower than those in blank control group and NC mimic group(P＜0.05).The expression of CyncliD1，CDK4，CDK6 in A549 cells of miR?1269 inhibitor group weresignificantly lower than those in blank control group and NC inhibitor group，the expression of p21Cip1 and p27Kip1 were significantly higher than those in blank control group and NC inhibitor group(P＜0.05).Conclusion The high expression of miR?1269 is relat? ed to the pathological changes of NSCLC，and miR?1269 can target the expression of cell cycle protein. |
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