| 王鹏,王胜文,宋晓红.同源盒 A9和前 B细胞白血病同源盒基因在结肠癌中的表达及意义[J].安徽医药,2020,24(11):2261-2264. |
| 同源盒 A9和前 B细胞白血病同源盒基因在结肠癌中的表达及意义 |
| Expressions and significances of transcription factors HOXA9 and PBX3 in colon cancer |
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| DOI：10.3969/j.issn.1009?6469.2020.11.037 |
| 中文关键词: 结肠肿瘤 前体 B细胞淋巴母细胞白血病淋巴瘤 基因，同源盒 同源盒 A9 前 B细胞白血病同源盒基因 3 预后 |
| 英文关键词: Colonic neoplasms Precursor B?cell lymphoblastic leukemia?lymphoma Genes,homeobox Homologous box A9 Homologous box gene 3 of pre?B?cell leukemia Prognosis |
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| 中文摘要: |
| 目的观察同源盒 A9(HOXA9)和前 B细胞白血病同源盒基因(PBX3)在结肠癌组织及其癌旁组织中的表达，探讨二者与结肠癌临床病理参数的关系及其对结肠癌 3年预后的影响。方法选取 2014年 8月至 2016年 5月于南阳市第一人民医院进行手术治疗的结肠癌病人 77例，取其癌组织及相应癌旁组织，实时荧光定量 PCR(qRT?PCR)法检测结肠癌及其癌旁组织 HOXA9 mRNA、PBX3 mRNA表达情况，免疫组织化学法检测其癌组织及癌旁组织标本中 HOXA9P与 PBX3蛋白表达，分析 HOXA9P、PBX3表达与临床病理特征的关系，运用 Spearman法分析 HOXA9P与 PBX3表达相关性，通过 Kaplan?Meier法对结肠癌病人 3年内存活情况进行分析。结果与癌旁组相比，结肠癌组 HOXA9 mRNA［(3.09±0.55)比(1.02±0.15)］、 PBX3 mRNA表达水平［(2.51±0.47)比(0.98±0.11)］、 HOXA9、PBX3蛋白阳性表达率显著升高(P＜0.05)； HOXA9、PBX3表达均与结肠癌病人分化程度、 TNM分期有关(P＜0.05)； Spearman相关性分析结果显示结肠癌组织中 HOXA9与 PBX3 mRNA表达呈正相关(rs＝0.643，P＜0.05)； Kaplan?Meier法分析显示 HOXA9、PBX3阳性表达组 3年内总生存率(OS)显著低于阴性表达组(41.2% VS 84.6%，43.6% VS 86.4%)。结论结肠癌组织中 HOXA9、PBX3均高表达，二者呈正相关，均与结肠癌分化程度、 TNM分期及预后相关，可能共同参与结肠癌的发生发展。 |
| 英文摘要: |
| Objective To observe the expressions of homologous box A9(HOXA9)and pre?B?cell leukemia homologous box 3(PBX3)in colon cancer and its adjacent tissues，and to explore their relationships with clinicopathological parameters of colon can?cer and their effects on three?year prognosis of colon cancer.Methods 77 patients with colon cancer who underwent surgical treat? ment in Nanyang First People’s Hospital from August 2014 to May 2016 were selected，cancer tissues and corresponding adjacent tissues were taken，real?time fluorescence quantitative PCR(qRT?PCR)was used to detect the expressions of HOXA9 mRNA and PBX3 mRNA in colon cancer and adjacent tissues，the expressions of HOXA9P and PBX3 were detected by immunohistochemistry， the relationships between the expressions of HOXA9P and PBX3 and clinicopathological features were analyzed，Spearman method was used to analyze the correlation between HOXA9P and PBX3 expressions，the 3?year survival of patients with colon cancer was analyzed by Kaplan?Meier method.Results Compared with the adjacent group，the expression levels of HOXA9 mRNA［(3.09± 0.55)vs.(1.02±0.15)］PBX3 mRNA［(2.51±0.47)vs.(0.98±0.11)］，HOXA9 and PBX3 proteins were significantly higher in colon cancergroup(P＜0.05).，The expressions of HOXA9 and PBX3 were related to the differentiation degree and TNM stage of colon cancer patients(P＜0.05).Spearman correlation analysis showed that HOXA9 was positively correlated with PBX3 mRNA expres? sions in colon cancer(rs＝0.643，P＜0.05).Kaplan?Meier analysis showed that the 3?year overall survival(OS)rate of HOXA9 and PBX3 positive expression group was significantly lower than that of negative expression group(41.2% vs.84.6%，43.6% vs.86.4%). Conclusion HOXA9 and PBX3 are highly expressed in colon cancer tissues，there is a positive correlation between them，and they are correlated with the degree of differentiation，TNM stage and prognosis of colon cancer，and may participate in the occur?rence and development of colon cancer. |
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