| 罗思佳,袁野,张建新,等.微小 RNA-20a、微小 RNA-92a在乳腺癌诊断及预后评估中的应用[J].安徽医药,2022,26(2):261-265. |
| 微小 RNA-20a、微小 RNA-92a在乳腺癌诊断及预后评估中的应用 |
| Application of microRNA-20a and microRNA-92a in the evaluation of breast cancer diagnosis and prognosis |
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| DOI：10.3969/j.issn.1009-6469.2022.02.013 |
| 中文关键词: 乳腺肿瘤 血清 微小 RNA-20a 微小 RNA-92a |
| 英文关键词: Breast neoplasms Serum MiR-20a MiR-92a |
| 基金项目: |
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| 中文摘要: |
| 目的分析微小 RNA(miR)-20a、miR-92a在乳腺癌病人血清中的表达情况，并探讨其对乳腺癌诊断及预后评估的价值。方法选取 2012年 1月至 2013年 5月中国人民解放军中部战区总医院首次确诊为乳腺癌并经手术治疗的病人 89例为观察组，以 80例同期健康体检者为健康对照组，采用实时荧光定量逆转录聚合酶链式反应( qRT-PCR)检测乳腺癌病人术前和术后及健康对照组血清 miR-20a和 miR-92a表达水平，分析 miR-20a和 miR-92a表达与临床病理特征的关系。 Spearman相关性分析血清 miR-20a和 miR-92a表达相关性， ROC曲线分析 miR-20a、miR-92a以及二者联合检测对乳腺癌的诊断价值， Kaplan-Meier法分析 miR-20a和 miR-92a表达对病人 5年总生存期( OS)影响， logistic回归分析影响乳腺癌预后的因素。结果乳腺癌病人血清 miR-20a［术前( 1.76±0.15)、术后( 1.38±0.12)比( 1.02±0.08)］和 miR-92a水平［术前( 1.83±0.13)、术后( 1.45±0.11)比( 1.11± 0.07)］较健康对照组均显著升高( P<0.05)。 miR-20a和 miR-92a表达与病人肿瘤分化程度、 TNM分期及淋巴结转移相关( P<0.05)。乳腺癌病人血清 miR-20a和 miR-92a表达呈线性正相关( r= 0.52，P<0.05)。 miR-20a和 miR-92a二者联合诊断乳腺癌的敏感度、特异度以及 AUC均显著升高( P<0.05)。 miR-20a和 miR-92a低表达病人 5年 OS均显著高于高表达病人［miR-20a(93.24%比 73.28%)、 miR-92a(94.85%比 74.57%)P<0.05］。 miR-20a和 miR-92a、分化程度、淋巴结转移均是影响乳腺癌病人预后的独立危险因素( P<0.05)。结论乳腺癌病血清中 miR-20a和 miR-92a表达异常，与病人肿瘤分化程度、 TNM分期及淋人，巴结转移相关，可能用于乳腺癌的诊断和预后判断。 |
| 英文摘要: |
| Objective To analyze the expression of microRNA-20a and microRNA-92a in serum of patients with breast cancer, and to explore their value in diagnosis and prognosis evaluation of breast cancer.Methods Eighty-nine patients with breast cancer whowere diagnosed for the first time and treated surgically in Central Theater General Hospital of PLA from January 2012 to May 2013were selected as the study subjects, and 80 healthy people in the same period were taken as the control group, the levels of serum miR-20a and miR-92a in patients with breast cancer before and after operation and in control group were detected by qRT-PCR, the relation. ships between the expressions of miR-20a and miR-92a with clinicopathological features were analyzed. Spearman correlation analysiswas used to analyze the correlation between the expressions of serum miR-20a and miR-92a, ROC curve was used to analyze the diag. nostic values of miR-20a, miR-92a, and their combined detection in breast cancer, Kaplan-Meier method was used to analyze the ef. fects of the expressions of miR-20a and miR-92a on patients' 5-year disease-free survival (OS), Logistic regression analysis was used to analyze the prognostic factors of breast cancer.Results The levels of serum miR-20a [before operation (1.76±0.15), after operation (1.38±0.12) vs. (1.02±0.08)] and miR-92a [before operation (1.83±0.13), after operation (1.45±0.11) vs. (1.11±0.07)] in breast cancer pa. tients were significantly higher than those in control group (P<0.05). The expressions of miR-20a and miR-92a were correlated with tu. mor differentiation, TNM stage, and lymph node metastasis (P<0.05). There was a positive correlation between the expressions of serum miR-20a and miR-92a in breast cancer patients (r=0.524, P<0.05). The sensitivity, specificity, and AUC of the combination of miR-20a and miR-92a in the diagnosis of breast cancer were significantly increased (P<0.05). The 5-year OS of patients with low expressions of miR-20a and miR-92a was significantly higher than that of patients with high expression of miR-92a [miR-20a (93.24% vs. 73.28%), miR-92a (94.85% vs. 74.57%), P<0.05]. miR-20a and miR-92a, differentiation degree and lymph node metastasis were independent risk factors affecting the prognosis of breast cancer patients (P<0.05).Conclusion The expressions of serum miR-20a and miR-92a are abnormal in breast cancer patients, they are related to the degree of differentiation of tumors, TNM stage, and lymph node metasta.sis, which may be used in the diagnosis and prognosis of breast cancer. |
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