| 代文意.锚蛋白 B在鼻咽癌组织中的表达及其对鼻咽癌细胞增殖、迁移和侵袭的影响[J].安徽医药,2022,26(2):347-350. |
| 锚蛋白 B在鼻咽癌组织中的表达及其对鼻咽癌细胞增殖、迁移和侵袭的影响 |
| Expression of ANK2 in nasopharyngeal carcinoma and its effects on proliferation, migration and invasion of nasopharyngeal carcinoma cells |
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| DOI：10.3969/j.issn.1009-6469.2022.02.033 |
| 中文关键词: 鼻咽肿瘤 锚蛋白 B 细胞增殖 迁移 侵袭 |
| 英文关键词: Nasopharyngeal neoplasms Ankyrin B Cell proliferation Migration Invasion |
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| 中文摘要: |
| 目的探讨锚蛋白 B(ANK2)基因在鼻咽癌组织中的表达及其对鼻咽癌细胞增殖、迁移及侵袭的影响。方法选取 2015年 10月至 2018年 10月南阳市中心医院存档的鼻咽癌组织 67例，实时荧光定量逆转录聚合酶链式反应( qRT-PCR)法检测人组织中 ANK2 mRNA表达，以 50例鼻咽部炎症组织作为对照组，分析鼻咽癌组织中 ANK2 mRNA表达与临床病理特征关系。以人永生化鼻咽上皮细胞 NP69为对照， RT-qPCR法检测鼻咽癌细胞系 6-10B和 5-8F中 ANK2 mRNA表达。转染 ANK2小干扰 RNA至 5-8F细胞， RT-qPCR检测细胞中 ANK2 mRNA表达水平验证转染效果，四甲基偶氮唑盐比色法( MTT)、 Transwell、蛋白质印迹法( Western blotting)分别检测干扰 ANK2表达对 5-8F细胞增殖、迁移和侵袭及磷酸化糖原合成酶激酶 3β(p-GSK3βser9)、 β-连环蛋白( β-catenin)和 c-myc蛋白表达的影响。结果与鼻咽部炎症组织比较，鼻咽癌组织中 ANK2 mRNA表达升高［(2.57±0.19)比( 1.05±0.08)t=53.119，P<0.001］。 ANK2高表达与鼻咽癌病人肿瘤分化程度、 TNM分期和淋巴结转移密切相关( P<0.05)。与 NP69细胞比较，鼻，咽癌细胞系 6-10B和 5-8F中 ANK2 mRNA表达升高［(2.34±0.16)、(2.95±0.21)比( 1.06± |
| 英文摘要: |
| Objective To investigate the expression of ankyrin B (ANK2)gene in nasopharyngeal carcinoma and its effects on prolif.eration, migration and invasion of nasopharyngeal carcinoma cells.Methods A total of 67 nasopharyngeal carcinoma tissues were col.lected from Nanyang Central Hospital from October 2015 to October 2018. The expression of ANK2 mRNA in human tissues was de.tected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), and 50 nasopharyngeal inflammation tis.sues were used as control group. The relationship between the expression of ANK2 mRNA in nasopharyngeal carcinoma tissues andclinicopathological characteristics was analyzed by chi-square test. Using human immortalized nasopharyngeal epithelial cell NP69 as a control, RT-qPCR was used to detect the expression of ANK2 mRNA in nasopharyngeal carcinoma cell lines 6-10B and 5-8F. ANK2 small interfering RNA was transfected into 5-8F cells, and then the expression of ANK2 mRNA was detected by RT-qPCR to verify thetransfection effect, and MTT, Transwell, and Western blot were used to detect the effects of interfering with ANK2 expression on 5-8F cell proliferation, migration and invasion, and the expression levels of p-GSK3βser9, β-cateninand c-myc protein.Results Comparedwith nasopharynx inflammatory tissue, the expression level of ANK2 mRNA was increased in nasopharyngeal carcinoma [(2.57±0.19) vs. (1.05±0.08), t=53.119, P<0.001]. The high expression of ANK2 was closely related to tumor differentiation, TNM stage and lymphnode metastasis in patients with nasopharyngeal carcinoma (P<0.05). Compared with the NP69 cells, the expression of ANK2 mRNA was increased in nasopharyngeal carcinoma cell lines 6-10B and 5-8F [(2.34±0.16), (2.95±0.21) vs. (1.06±0.09), P<0.001]. After inter. ference with ANK2 expression, 5-8F cell survival rate, number of migrated and invaded cells, and the protein expression of p-GSK3βser9, β-catenin and c-myc were significantly decreased (P<0.001).Conclusion The expression of ANK2 gene were increasedin nasopharyngeal carcinoma tissues and cell lines. Interfering with ANK2 could hinder the proliferation, migration and invasion of na.sopharyngeal carcinoma cells by inhibiting the Wnt/β-catenin signaling pathway. |
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