| 刘菲菲,席照亮,刘秋月.原花青素提高人舌癌细胞系放射敏感性及机制研究[J].安徽医药,2022,26(3):443-447. |
| 原花青素提高人舌癌细胞系放射敏感性及机制研究 |
| Effect and mechanism of grape seed proanthocyanidin on improving radiosensitivity of tongue cancer cells |
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| DOI：10.3969/j.issn.1009-6469.2022.03.005 |
| 中文关键词: 舌肿瘤 原花青素 Tca8113(人舌鳞癌细胞) 放射敏感性 微小 RNA-124 细胞凋亡 |
| 英文关键词: Tongue neoplasms Grape seed proanthocyanidins Tca8113 (human tongue cancer cells) Radiosensitivity MiR124 Apoptosis |
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| 中文摘要: |
| 目的探讨原花青素( grape seed proanthocyanidins，GSP)提高人舌癌细胞系放射敏感性及机制。方法 2019年 3月至 2020年 2月，将对数期生长的人舌癌 Tca8113细胞分为对照组( Tca CON)，Tca8113细胞加 10 mg/L GSP组( Tca GSP-10 mg/L)、 Tca8113细胞加 20 mg/L GSP组( Tca GSP-20 mg/L)、 Tca8113细胞照射组( Tca IR)、 Tca8113细胞照射加 10 mg/L GSP组( Tca IR+ GSP-10 mg/L)、 Tca8113细胞照射加 20 mg/L GSP组( Tca IR+GSP-20 mg/L)。MTT法检测加入不同浓度 GSP对舌癌细胞 Tca8113增殖的影响；流式细胞术检测 6 MV X线直线加速器照射后( 6 Gy)GSP对 Tca8113细胞凋亡的影响；实时荧光定量逆转录聚合酶链反应(qRT-PCR)实验检测在加入不同浓度 GSP作用下对照射后 Tca8113细胞微小 RNA(miR)-124表达的影响；蛋白质印迹法(Western blotting)检测加入不同浓度 GSP作用下对照射后 Tca8113细胞 B细胞淋巴瘤 -2(Bcl-2)和胱天蛋白酶 -9(caspase-9)蛋白水平的影响。结果 MTT法结果显示， Tca IR组、 Tca IR+GSP-10 mg/L组及 Tca IR+GSP-20 mg/L组 Tca8113细胞存活率分别为( 63.21±2.97)%、(41.19±4.33)%及( 19.61±2.51)%，GSP加强了照射后对人舌癌 Tca8113细胞的抑制，且随药物浓度的增大，抑制效果增强( F=126.30，P<0.001)；流式细胞术结果显示， Tca IR组、 Tca IR+GSP-10 mg/L组及 Tca IR+GSP-20 mg/L组 Tca8113细胞凋亡率分别为( 7.62±0.27)%、(9.61±0.42)%及( 17.50±0.37)%，GSP显著提高了照射后 Tca8113细胞的凋亡率( F=55.77，P= |
| 英文摘要: |
| Objective To investigate the effect and mechanism of proanthocyanidin on improving radiosensitivity of tongue cancer cells.Methods From March 2019 to February 2020, human tongue cancer Tca8113 cells growing in logarithmic phase were assignedinto control group (Tca CON), Tca8113 cells plus 10 mg/L GSP group (Tca GSP-10 mg/L), Tca8113 cells plus 20 mg/L GSP group (Tca GSP-20 mg/L), Tca8113 cell irradiation group (Tca IR), Tca8113 cell irradiation plus 10 mg/L GSP group (Tca IR + GSP-10 mg/L), and Tca8113 cell irradiation plus 20 mg/L GSP group ( Tca IR + GSP-20 mg/L). MTT experiments were performed to detect the effects ofGSP with different concentrations on the proliferation of Tca8113cells; flow cytometry was performed to detect the effects of GSP after 6MV X-ray linear accelerator irradiation (6 Gy) on the apoptosis of Tca8113 cells; real-time fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments were performed to detect the effects of GSP with different concentrations on the expression of miR-124 in Tca8113 cells after irradiation; the Western blotting test was performed to detect the effects of GSP with different concentrations on the levels of Bcl-2 and caspase-9 protein in Tca8113 cells after irradiation.Results MTT results showed that the survival rates of Tca8113 cells in Tca IR group, Tca IR+GSP-10 mg/L group and Tca IR+GSP-20 mg/L group were (63.21±2.97)%,(41.19±4.33)% and (19.61%±2.51)%. GSP enhanced the inhibition of human tongue cancer Tca8113 cells after irradiation, and the inhibitory effect was enhanced with the increase in drug concentration (F=126.30, P<0.001). The results of flow cytometry showed that |
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