| 张欢欢,王娟,李超.血清维生素 D水平和维生素 D受体基因多态性与肥胖型哮喘儿童的相关性分析[J].安徽医药,2022,26(3):564-568. |
| 血清维生素 D水平和维生素 D受体基因多态性与肥胖型哮喘儿童的相关性分析 |
| A correlation analysis of serum vitamin D level and vitamin D receptor gene polymorphism with obese children with asthma |
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| DOI：10.3969/j.issn.1009-6469.2022.03.034 |
| 中文关键词: 哮喘 儿童肥胖 肥胖型哮喘 维生素 D受体 基因多态性 |
| 英文关键词: Asthma Pediatric obesity Obese asthma Vitamin D receptor Gene polymorphism |
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| 中文摘要: |
| 目的研究血清维生素 D水平和维生素 D受体( VDR)基因多态性与肥胖型哮喘儿童的相关性。方法前瞻性分析 2016年 10月至 2019年 10月在驻马店市第一人民医院小儿呼吸内科收治的非肥胖型哮喘 96例、肥胖型哮喘 45例、健康对照组儿童 54例，收集儿童的一般临床资料；酶联免疫吸附测定检测外周血中炎性因子白介素( IL)-4、IL-7、IL-10、IL-17、IL-33和 25羟维生素 D［25(OH)D］水平；检测受试者肺功能指标；全血基因组 DNA抽提及检测 VDR基因型 rs731236、rs1544410、 rs1168271和 rs2228570位点多态性； logistic回归模型分析影响肥胖型哮喘儿童发病的相关基因型。结果肥胖型哮喘组外周血中炎性因子 IL-4、IL-7、IL-10、IL-17和 IL-33与对照组比较均差异有统计学意义，肥胖型哮喘组外周血中 25(OH)D(18.6±3.2) μg/L低于非肥胖型哮喘组(23.1±3.5)μg/L和对照组( 35.6±6.5)μg/L(P<0.05)。三组间第 1秒用力呼气容积( FEV1)、用力肺活量(FVC)、一秒率、呼气流量峰值( PEF)和残气量 /肺活量( RV/TLV)比较均差异有统计学意义，且肥胖型哮喘组 FEV1一秒率低于非肥胖型哮喘组和对照组， RV/TLV高于非肥胖型哮喘组和对照组， FVC和 PEF低于对照组(均 P<0.05)。三组儿童、VDR基因型 rs2228570位点 AA、AG、GG基因型和 A、G基因频率分布差异有统计学意义。 rs2228570位点 AA基因型为肥胖型哮喘发病的危险因素( OR=1.365，95%CI:1.024~1.748，P=0.028)GG基因型为保护性因素( OR=0.351，95%CI:0.026~0.514，P=0.002)。结论 25(OH)D水平在肥胖型哮喘组中水平降低， VDR基，因 rs2228570位点 AA基因型为肥胖型哮喘的危险因素， GG基因型为保护性因素。 |
| 英文摘要: |
| Objective To study the correlation between serum vitamin D level and vitamin D receptor (VDR) gene polymorphismwith obese asthmatic children.Methods Prospective analysis was performed of 96 cases of non-obese asthma, 45 cases of obese asthma, who were admitted to Department of Pediatric Respiratory Medicine, Zhumadian First People′s Hospital from October 2016 to October 2019, and 54 cases of healthy control children. General clinical data of the children were collected. Enzyme-linked immunosorbent assay was performed to detect the levels of inflammatory factors interleukin (IL)-4, IL-7, IL-10, IL-17, IL-33 and 25-hydroxyvitamin D [25(OH)D] in peripheral blood. Pulmonary function indicators of the subjects were tested. Whole blood genomic DNA extractionand detection of vitamin D receptor genotype rs731236, rs1544410, rs1168271 and rs2228570 loci polymorphism were performed. Logistic regression model was used to analyze the genotypes associated with asthma in obese children. Results Compared with the control group, the levels of the inflammatory factors IL-4, IL-7, IL-10, IL-17 and IL-33 in the peripheral blood of the obese asthma groupwere significantly different. Peripheral blood 25(OH)D level in the obese asthma group was lower than that in non-obese asthma group and control group [(18.6±3.2) μg/L vs. (23.1±3.5) μg/L vs. (35.6±6.5) μg/L. P<0.05]. Forced expiratory volume in 1 second (FEV1),forced vital capacity (FVC), rate in one second, peak expiratory flow (PEF) and residual capacity/vital capacity (RV/TLV) among thethree groups showed statistically significant difference; of the obese asthma group the FEV1, rate in one second were lower than those of the non-obese asthma group and the control group, the RV/TLV was higher than those of the non-obese asthma group and the control group, and the FVC and PEF were lower than those of the control group (all P<0.05). There was a statistically significant difference inthe frequency distribution of AA, AG, GG genotypes and A and G genotypes of vitamin D receptor genotype rs2228570 in three groups of children. The rs2228570 AA genotype was a risk factor for asthma of obesity children (OR=1.365, 95%CI: 1.024-1.748, P=0.028), and the GG genotype was a protective factor (OR=0.351, 95%CI: 0.026-0.514, P=0.002).Conclusions The level of 25-hydroxyvitaminD is reduced in obese asthma group. AA genotype of rs2228570 in vitamin D receptor gene is a risk factor for obese asthma, and GGgenotype is a protective factor. |
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