| 金肇权,张文彬,陈欣,等.CD14-TLR4-NF-κB信号传导通路在脂多糖诱导ALI/ARDS中的作用及机制研究[J].安徽医药,2022,26(4):643-647. |
| CD14-TLR4-NF-κB信号传导通路在脂多糖诱导ALI/ARDS中的作用及机制研究 |
| Role and mechanism of cd14-tlr4-nf -κB signaling pathway in LPS induced ALI / ARDS |
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| DOI：10.3969/j.issn.1009-6469.2022.04.002 |
| 中文关键词: 急性肺损伤 呼吸窘迫综合征，成人 白细胞分化抗原 14 Toll样受体 4 核因子激活的 B细胞的 κ-轻链增强 信号传导通路 脂多糖 大鼠， Sprague-Dawley |
| 英文关键词: Acute lung injury Respiratory distress syndrome, adult Leukocyte differentiation antigen 14 Toll like receptor 4 Enhancement of kappa light chain in nuclear factor activated B cells Signal transduction pathway Lipopolysaccharide Rats,Sprague-Dawley |
| 基金项目:江苏省第五期 “333工程”培养项目( KY201711) |
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| 中文摘要: |
| 目的探讨白细胞分化抗原 14(CD14)-Toll样受体 4(TLR4)-核因子激活的 B细胞的 κ-轻链增强( NF-κB)信号传导通路在脂多糖( LPS)诱导急性肺损伤 /急性呼吸窘迫综合征( ALI/ARDS)中作用及机制。方法于 2020年 1—12月，选取购自北京宝元兴业科技有限公司的清洁级健康雄性 SD大鼠 90只，分为 A、B、C三组，每组各 30只， C组为正常大鼠模型组， B组为 ALI/ ARDS模型组大鼠， A组为 ALI/ARDS＋CD14、TLR4以及 NF-κB模拟物模型组大鼠，比较三组大鼠相关指标的差异。结果造模 12 h后， A、B、C三组大鼠的呼吸频率( RR)分别是( 127.92±17.04)次 /分、(87.51±8.42)次 /分、(55.28±3.73)次 /分， A组>B组>C组，动脉血氧分压( PaO2)分别是( 7.63±1.16)mmHg、(10.58±1.65)mmHg、(13.46±2.05)mmHg，A组B组>C组，均差异有统计学意义( P<0.05)。 Pearson相关性分析显示， A组大鼠的脂多糖蛋白分别与 CD14蛋白、 TLR4蛋，白以及 NF-κB P65蛋白均呈正相关( P<0.05)。结论 CD14-TLR4NF-κB信号传导通路能够增强脂多糖表达并促进机体炎性活动，参与 ALI/ARDS的发生发展。 |
| 英文摘要: |
| Objective To investigate the role and mechanism of leukocyte differentiation antigen 14 (CD14) -Toll like receptor 4 (TLR4) -nuclear factor-activated κ-light chain enhancement ( NF -κB) signaling pathway in lipopolysaccharide (LPS) induced acute lung injury / acute respiratory distress syndrome (ALI/ARDS).Methods From January 2020 to December 2020, 90 SPF SD rats purchasedfrom Beijing Baoyuan Xingye Technology Co., LTD were assigned into three groups: A, B and C group, 30 rats in each group. Group C wasthe normal rat model group, group B was the ALI / ARDS model group, group A was the ALI / ARDS + CD14, TLR4 and NF -κB mimic model group. The differences of related indexes among the three groups were compared.Results 12 hours after the establishment of the model, the respiratory rate (RR) of rats in groups A, B and C was (127.92±17.04) times/min, (87.51±8.42) times/min, and (55.28±3.73)times/min, respectively, group A > group > group C; the arterial partial blood oxygen pressure (PaO2) was (7.63±1.16) mmHg, (10.58±1.65) mmHg, and (13.46±2.05) mmHg, group A < group B < group C; and the difference was statistically significant (P<0.05). The expres sion level of CD14 protein in group A, B and C was (0.94±0.23), (0.69±0.18) and (0.48±0.13), respectively, TLR4 protein was (0.97±0.26), (0.65±0.13) and (0.43±0.11), respectively, NF-κB P65 protein was (1.03±0.28), (0.67±0.17) and (0.47±0.12), respectively, lipidpolyglycol protein was (0.98±0.27), (0.66±0.16) and (0.45±0.12), respectively, group A > group B > group C, and the differences were sta tistically significant (P<0.05). Pearson correlation analysis showed that LPS protein in group A was positively correlated with CD14 pro tein, TLR4 Protein and NF -κB p65 protein, respectively (P < 0.05).Conclusion CD14-TLR4-NF-κB signaling pathway can enhancethe expression of LPS and promote inflammatory activity of the body, and participate in the development of ALI / ARDS. |
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