| 秦小菀,高伟霞,陈朴.长链非编码 RNA H19在肺炎支原体肺炎病儿血清中的表达及其意义[J].安徽医药,2022,26(4):805-808. |
| 长链非编码 RNA H19在肺炎支原体肺炎病儿血清中的表达及其意义 |
| Expression and significance of long noncoding RNA H19 in the serum of children with mycoplasma pneumoniae pneumonia |
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| DOI：10.3969/j.issn.1009-6469.2022.04.037 |
| 中文关键词: 肺炎，支原体 长链非编码 RNA H19 肺炎支原体脂质相关膜蛋白(LAMPS) 肺泡巨噬细胞 凋亡 炎症 |
| 英文关键词: Pneumonia, mycoplasma LncRNA H19 Mycoplasma pneumoniae lipid-associated membrane protein (LAMPS) Alveolar macrophages Apoptosis Inflammation |
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| 目的探讨长链非编码 RNA H19(LncRNA H19)在肺炎支原体肺炎( MPP)病儿血清中的表达及其对肺炎支原体脂质相关膜蛋白( LAMPS)诱导的肺泡巨噬细胞凋亡和炎性因子表达的影响。方法收集 2017年 10月至 2018年 12月南阳市中心医院收治的 MPP病儿 60例为观察组，根据病儿病情程度分为恢复期( 30例)与急性期( 30例)；选取同期到我院体检的健康儿童 60例作为对照组。实时荧光定量聚合酶链反应( qRT-PCR)检测 LncRNA H19的表达水平；采用受试者工作特征( ROC)曲线分析 LncRNA H19对 MPP的诊断价值。体外培养小鼠肺泡巨噬细胞 MH-S，LAMPS处理细胞建立细胞损伤模型。分别将 si-NC、 si-H19转染至 MH-S细胞，使用 LAMPS处理 24 h。流式细胞术检测细胞凋亡率； ELISA法检测肿瘤坏死因子 -α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素 6(IL-6)水平。结果与对照组相比，观察组血清 LncRNA H19的表达水平显著升高［(1.01±0.13)比 |
| 英文摘要: |
| Objective To investigate the expression of long noncoding RNA H19 (lncRNA H19) in the serum of children with My coplasma pneumoniae pneumonia (MPP) and its effect on mycoplasma pneumoniae lipid-associated membrane protein (LAMPS)-in duced alveolar macrophage apoptosis and inflammatory factor expression.Methods A total of 60 children with MPP who were admit ted to Nanyang Central Hospital from October 2017 to December 2018 were collected as the observation group. According to the de gree of the illness of the children, they were divided into convalescent stage (30 cases) and acute stage (30 cases). Sixty healthy chil dren who underwent physical examination in our hospital during the same period were selected as the control group. Real-time quanti tative polymerase chain reaction (qRT-PCR) was used to detect the expression level of LncRNA H19. A receiver operating characteris tic (ROC) curve was used to analyze the diagnostic value of LncRNA H19 for MPP. The MH-S mouse alveolar macrophages were cul tured in vitro, and the cells were treated with LAMPS to establish a cell injury model. si-NC and si-H19 were transfected into MH-S cells and treated with LAMPS for 24 h. Flow cytometry was used to detect the apoptosis rate. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).Results Compared with the control group, the expression level of serum LncRNA H19 in the observation group was significantly increased[(1.01±0.13) vs. (2.58±0.25), P < 0.05]. Compared with the MPP recovery period, the expression level of serum LncRNA H19 in theacute phase of MPP was significantly increased [(1.63±0.20) vs. (3.24±0.39), P < 0.05]. ROC analysis showed that the sensitivity of ln cRNA H19 in the diagnosis of MPP was 86.67%, the specificity was 86.67%, and the AUC was 0.869. Compared with the controlgroup, the LAMPS group had a significantly increased apoptosis rate and TNF-α, IL-1β and IL-6 levels [(8.24±1.35)% vs. (26.57±3.24)%, (7.23±2.12) pg/mL vs. (26.32±6.47) pg/mL, (11.62±3.21) pg/mL vs. (30.24±6.23) pg/mL, (11.20±3.21) vs. (45.32±12.16) pg/ mL, P < 0.05]. Compared with the si-NC group, the apoptosis rate and TNF-α, IL-1β and IL-6 levels of the si-H19 group were signifi cantly reduced (P < 0.05).Conclusion LncRNA H19 is highly expressed in the serum of children with MPP, and can inhibit LAMPS induced apoptosis and the inflammatory response of alveolar macrophages. |
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