| 贾龙江,郝斌,许长宝,等.长链非编码 RNA FOXD2相邻相反链 RNA 1靶向调控微小 RNA-760对前列腺癌细胞增殖和凋亡的影响[J].安徽医药,2022,26(6):1183-1187. |
| 长链非编码 RNA FOXD2相邻相反链 RNA 1靶向调控微小 RNA-760对前列腺癌细胞增殖和凋亡的影响 |
| Targeted-regulating of miR-760 by lncRNA FOXD2 adjacent opposite strand RNA 1 and its impact on proliferation and apoptosis of prostate cancer cells |
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| DOI：10.3969/j.issn.1009-6469.2022.06.029 |
| 中文关键词: 前列腺肿瘤 FOXD2相邻相反链 RNA 1 微小 RNA-760 增殖 凋亡 |
| 英文关键词: Prostatic neoplasms FOXD2-AS1 MiR-760 Proliferation Apoptosis |
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| 中文摘要: |
| 目的探讨 FOXD2相邻相反链 RNA 1(FOXD2-AS1)对前列腺癌细胞的增殖和凋亡的影响以及作用机制。方法验于 2019年 1—11月进行，根据 DU145细胞转染情况分为空载体质粒( pcDNA)组、 FOXD2-AS1过表达质粒( pcDNA-FOXD2实AS1)组、小干扰 RNA阴性对照( si-NC)组、 FOXD2-AS1小干扰 RNA(si-FOXD2-AS1)组、微小 RNA(miR)-760组、 miR-760阴性对照( miR-NC)组及 si-FOXD2-AS1+抗 miR-760阴性对照( anti-miR-NC)组、 si-FOXD2-AS1+抗 miR-760(anti-miR-760)组。实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测 miR-760和 FOXD2-AS1表达水平；双荧光素酶报告基因实验检测荧光活性；蛋白质印迹法检测蛋白表达； MTT法检测细胞增殖；流式细胞术检测细胞凋亡。结果与正常前列腺上皮细胞 RWPE-1相比，前列腺癌细胞 DU145、LNCaP、22Rv1中 miR-760表达水平显著降低［( 0.34±0.03)、(0.56±0.05)、(0.42±0.04)比( 1.01±0.08)］(P< |
| 英文摘要: |
| Objective To investigate the impact of FOXD2 adjacent opposite strand RNA 1 (FOXD2-AS1) on proliferation and apoptosis of prostate cancer cells and its mechanism.Methods Experiments were conducted from January to November of 2019. According to respective ways of DU145 transfection, there were pcDNA group, pcDNA-FOXD2-AS1 group, si-NC group, si-FOXD2-AS1 group, miR-NC group, miR-760 group, si-FOXD2-AS1+anti-miR-NC group, and si-FOXD2-AS1+anti-miR-760 group. The expression levels of miR-760 and FOXD2-AS1 were detected by real-time quantitative polymerase chain reaction (qRT-PCR), fluorescence activity by double luciferase reporter gene assay, protein expression by Western blotting, cell proliferation by thiazole blue (MTT) assay, andapoptosis by flow cytometry.Results Compared with normal prostate epithelial cells RWPE-1, miR-760 expressions in DU145, LN‐CaP and 22Rv1 were significantly decreased [(0.34±0.03), (0.56±0.05), (0.42±0.04) vs. (1.01±0.08)] (P<0.001), while FOXD2-AS1 expressions were significantly increased [(2.28±0.23), (2.13±0.21), (2.45±0.24) vs. (1.00±0.09)] (P<0.001). Compared with miR-NC group, the DU145 luciflucase activity of WT-FOXD2-AS1 prostate cancer cells in the MiR-760 group was significantly decreased [(0.42±0.03) vs. (1.04±0.09)] (P<0.001). Compared with pcDNA group, the expression level of miR-760 in DU145 cells in PcDNAFOXD2-AS1 group was significantly decreased [(0.45±0.04) vs. (1.02±0.09)] (P<0.001). Compared with si-NC group, miR-760 expression level in DU145 cells in si-FOXD2-AS1 group was significantly increased [(2.34±0.23) vs. (1.01±0.08)] (P<0.001). Inhibition of FOXD2-AS1 expression and overexpression of miR-760 both inhibited the expressions of Bcl-2 and cyclin D1 proteins, promoted theexpressions of Bax and P21 proteins, inhibited the proliferation of DU145 cells, and promoted cell apoptosis. Inhibition of miR-760 overexpression could reverse and inhibit the effect of FOXD2-AS1 on DU145 proliferation inhibition and apoptosis promotion.Conclu? sion LncRNA FOXD2-AS1 may inhibit the proliferation and promote apoptosis of prostate cancer DU145 cells by targeting miR-760, which may provide a new target for the prevention and treatment of prostate cancer. |
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