| 苏涌,张蕊,刘加涛,等.高效液相色谱法测定肾移植病人泼尼松龙、伏立康唑及其氮氧化物浓度[J].安徽医药,2022,26(7):1320-1324. |
| 高效液相色谱法测定肾移植病人泼尼松龙、伏立康唑及其氮氧化物浓度 |
| Determination of prednisolone, voriconazole and voriconazole N-oxide in kidney transplant patients by high-performance liquid chromatography |
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| DOI：10.3969/j.issn.1009-6469.2022.07.011 |
| 中文关键词: 色谱法，高压液相 泼尼松龙 伏立康唑 伏立康唑氮氧化物 血药浓度 |
| 英文关键词: Chromatography,high-pressure liquid Prednisolone Voriconazole Voriconazole N-oxide Blood concentration |
| 基金项目:安徽省高校自然科学基金重点项目( KJ2019A0247)；吴阶平医学基金会临床科研专项资助基金( 320.6 750.2020-04-39)；安徽医科大学第一附属医院博士科研经费( BSKY2019009) |
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| 中文摘要: |
| 目的建立快速、灵敏的高效液相色谱法测定病人血浆泼尼松龙、伏立康唑及其代谢物伏立康唑氮氧化物的浓度。方法色谱柱为安捷伦 ZORBAX Eclipse XDB C18(4.6 mm×250 mm，5 μm)；流动相为乙腈∶水(27∶73～60∶40)梯度洗脱；流速为 1.0 mL/min；波长为 244 nm；柱温为 30 ℃。收集 2019年 9月至 2020年 3月在安徽医科大学第一附属医院接受伏立康唑治疗的肾移植病人 24例，检测 24例肾移植病人血药浓度，男 14例、女 10例。结果泼尼松龙、伏立康唑及其氮氧化物色谱峰分离良好。泼尼松龙在 3.13～100.00 μg/L范围内线性关系良好， r=0.993 1；伏立康唑在 0.16～20.00 mg/L范围内线性关系良好， r=0.999 8；伏立康唑氮氧化物在 0.16～10.00 mg/L范围内线性关系良好， r=0.997 3。三种化合物日内及日间 RSD在 1.93%～6.74%，绝对回收率在 |
| 英文摘要: |
| Objective To establish a rapid and sensitive high-performance liquid chromatography method for the determination ofplasma concentrations of prednisolone, voriconazole and voriconazole N-oxide in patients.Methods The chromatographic column was Agilent ZORBAX Eclipse XDB C18 (4.6 mm×250 mm, 5 μm); the mobile phase was acetonitrile-water (27:73-60:40) gradient elution;the flow rate was 1.0 mL/min; the wavelength was 244 nm; and the column temperature was 30 ℃. A total of 24 kidney transplant pa-tients who were hospitalized in the First Affiliated Hospital of Anhui Medical University and received voriconazole from September 2019to March 2020 were enrolled, including 14 males and 10 females, and their blood drug concentrations were monitored.Results The chro-matographic peaks of prednisolone, voriconazole and voriconazole N-oxide were well separated. The linear relationship of prednisolone was good in the range of 3.13-100.00 μg/L, r=0.993 1; the linear relationship of voriconazole was good in the range of 0.16-20.00 mg/L, r= 0.999 8; the linear relationship of voriconazole N-oxide was good in the range of 0.16-10.00 mg/L, r=0.997 3. The intraday and interdayRSDs of the three compounds ranged from 1.93% to 6.74%, and the absolute recoveries ranged from 65.48% to 86.68%. The concentra-tionofprednisolonein24patientswas7.75-72.75μg/L,theconcentrationofvoriconazolewas 0.17-5.44mg/L,andtheconcentrationofvori-conazoleN-oxidewas0.66-3.06mg/L, with large individual differences.Conclusion The method has strong specificity and high sensitiv-ity and is suitable for the determination of plasma concentrations of prednisolone, voriconazole, and voriconazole N-oxide. |
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