| 李国东,梁博,何明璇,等.炎症性肠病的微小 RNA-377、微小 RNA-31表达及其与肠道菌群相关性[J].安徽医药,2022,26(7):1325-1328. |
| 炎症性肠病的微小 RNA-377、微小 RNA-31表达及其与肠道菌群相关性 |
| Expression of miR-377 and miR-31 in inflammatory bowel disease and their correlation with intestinal flora |
| |
| DOI：10.3969/j.issn.1009-6469.2022.07.012 |
| 中文关键词: 炎性肠疾病 微小 RNA-377 微小 RNA-31 炎性反应 肠道菌群 白细胞介素 34 C反应蛋白 .比)、高，表， |
| 英文关键词: Inflammatory bowel diseases miR-377 miR-31 Inflammatory response Intestinal flora Interleukin 34 C-reactive protein |
| 基金项目:河北省保定市社发项目( 18ZF037) |
|
| 摘要点击次数: 1057 |
| 全文下载次数: 333 |
| 中文摘要: |
| 目的探讨微小 RNA-377(miR-377)、微小 RNA-31(miR-31)在炎症性肠病血清中的表达及其与肠道菌群相关性。方法选取 2016年 5月至 2019年 6月保定市第一中心医院收治的 110例炎症性肠病病人为研究组，其中活动期 69例、缓解期 41例，同时选取同院体检的健康志愿者 60例为对照组；采用 qRT-PCR法检测血清 miR-377、miR-31的表达量；根据表达量平均值将病人分为 miR-377低表达组 53例、 miR-377高表达组 57例、 miR-31低表达组 57例、 miR-31高表达组 53例，比较分析 miR-377、 miR-31表达量与肠道菌群［肠球菌( EC)、酵母菌( SB)、双歧杆菌( BL)、消化球菌( PS)、小梭菌( CD)、肠杆菌( EMB)、葡萄球菌(SP)、拟杆菌(BD)、乳杆菌(LC)、真杆菌(ES)］数量的相关性；采用 ELISA法检测血清白细胞介素 -34(IL-34)、C反应蛋白(CRP)的水平；采用电化学发光法检测血清降钙素原(PCT)的水平；采用 Pearson法分析 miR-377、miR-31表达量与 IL-34、CRP、PCT水平的相关性。结果与对照组比较，研究组血清中 miR-377、miR-31的表达水平显著升高［(0.96±0.16)(2.23±0.50)(0.97±0.24)比 |
| 英文摘要: |
| Objective To investigate the expression of microRNA-377 (miR-377) and miR-31 in the serum of inflammatory bowel disease (IBD) patients and their correlation with intestinal flora.Methods A total of 110 patients with IBD admitted to the First Cen-tral Hospital of Baoding from May 2016 to June 2019 were selected as the research group, including 69 patients in the active periodand 41 in the remission period, and 60 healthy volunteers who underwent physical examination in the same hospital were selected asthe control group. qRT-PCR was used to detect the expression levels of serum miR-377 and miR-31. According to the average expres-sion level, the patients were divided into 53 cases in the miR-377 low expression group, 57 cases in the miR-377 high expression group, 57 cases in the miR-31 low expression group, and 53 cases in the miR-31 high expression group. Analyze the correlations be-tween the levels of miR-377 and miR-31 and intestinal flora [Enterococcus (EC), Yeast (SB), Bifidobacterium (BL), Peptococcus (PS),Clostridium minor (CD), Enterobacter (EMB), Staphylococcus (SP), Bacteroides (BD), Lactobacillus (LC) and Eubacterium (ES)]. Thelevels of serum interleukin-34 (IL-34) and C-reactive protein (CRP) were detected by enzyme-linked immunosorbent assay (ELISA).The electrochemiluminescence method was used to detect the level of serum procalcitonin (PCT). The Pearson method was used to ana-lyze the correlation between the expression levels of miR-377 and miR-31 and the levels of IL-34, CRP and PCT.Results Compared with the control group, the expression levels of miR-377 and miR-31 in the serum of the study group were significantly increased [(0.96± 0.16) vs. (2.23±0.50), (0.97±0.24) vs. (2.39±0.41), (P < 0.05) ], the active period was significantly higher than the remission period [(1.70± 0.26) vs. (2.54±0.30), (2.02±0.19) vs. (2.61±0.34), (P < 0.05)], and the levels of serum IL-34, CRP, PCT were significantly increased (P< 0.05). miR-377 was positively correlated with IL-34, CRP and PCT levels (r=0.99, 0.50, 0.86, P<0.001). The expression level of miR-31 was positively correlated with the levels of IL-34, CRP and PCT (r = 0.95, 0.64, 0.85, P < 0.001). Compared with the miR-377 low expres-sion group, the number of SB, BL, LC, EC, BD, PS, and SC in the miR-377 high expression group were significantly increased (P < 0.05), and the number of ES was significantly decreased(P < 0.05). Compared with the miR-31 low expression group, the number of SB, BL, LC, EC, BD, PS, and SC in the miR-31 high expression group was significantly increased (P < 0.05), and the number of ES was significantly decreased (P<0.05).Conclusion The expression levels of miR-377 and miR-31 in the serum of IBD patients were increased, and both were related to the inflammatory response and intestinal flora. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
