| 孙贺军,孔永红,陈新蕾,等.没食子酰芍药苷对白细胞介素 8诱导的人脐静脉血管内皮细胞增殖和迁移的影响[J].安徽医药,2022,26(8):1510-1515. |
| 没食子酰芍药苷对白细胞介素 8诱导的人脐静脉血管内皮细胞增殖和迁移的影响 |
| Effect of 6'-O-Galloylpaeoniflorin on interleukin-8-induced proliferation and migration of human umbilical vein endothelial cells |
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| DOI：10.3969/j.issn.1009-6469.2022.08.006 |
| 中文关键词: 人脐静脉内皮细胞 内皮，血管 没食子酰芍药苷 微小 RNA-212-3p 激活转录因子 2 增殖 迁移 |
| 英文关键词: Human umbilical vein endothelial cells Endothelium, vascular 6'-O-Galloylpaeoniflorin(GPF) miR-212-3p Activating transcription factor 2 Proliferation Migration |
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| 中文摘要: |
| 目的研究没食子酰芍药苷(GPF)对白细胞介素 8(IL-8)诱导的人脐静脉血管内皮细胞( HUVECs)增殖和迁移的影响及潜在分子机制。方法用 100、200和 400 μmol/L GPF分别处理 100 μg/L IL-8诱导的 HUVECs，实时荧光定量逆转录聚合酶链反应( qRT-PCR)和蛋白质印迹法( Western blotting)分别检测 HUVECs细胞中 miR-212-3p、激活转录因子 2(ATF2)、增殖细胞核抗原( PCNA)和基质金属蛋白酶 -2(MMP-2)的表达，四氮唑蓝( MTT)和 Transwell实验分别测定 HUVECs细胞存活率和细胞迁移，双荧光素酶报告系统验证 miR-212-3p与 ATF2的调控关系。结果与对照 0 μmol/L组相比， 100 μmol/L、200 μmol/L和 400 μmol/L的 GPF可抑制 IL-8诱导的 HUVECs增殖和迁移，促进 miR-212-3p表达，抑制 ATF2表达，呈剂量依赖趋势；低表达 miR-212-3p可逆转 400 μmol/L GPF对 IL-8诱导的 HUVECs细胞增殖和迁移的抑制作用； miR-212-3p靶向 ATF2，调控 ATF2的表达；低表达 ATF2可部分逆转低表达 miR-212-3p对 GPF处理的 IL-8诱导的 HUVECs细胞增殖和迁移的作用。结论 GPF通过 miR-212-3p/ATF2途径抑制 IL-8诱导的 HUVECs细胞增殖和迁移。 |
| 英文摘要: |
| Objective To investigate the effects of 6'-O-Galloylpaeoniflorin(GPF) on proliferation and migration of human umbilical vein endothelial cells (HUVECs) induced by IL-8 and the underlying mechanism.Methods HUVECs induced by 100 μg/L of IL-8 were treated with GPF at 100, 200 and 400 μmol/L, respectively. The expression levels of miR-212-3p, ATF2, PCNA and MMP2 in HUVECs were detected by qRT-PCR and Western blotting. The survival rate and migration of HUVECs were determined by MTT andTranswell assay. The relationship between miR-212-3p and ATF2 was verified by dual-luciferase reporter assay system.Results Com- pared with control 0 μmol/L group, 100 μmol/L, 200 μmol/L and 400 μmol/L of GFP inhibited the proliferation and migration of HU-VECs induced by IL-8, promoted the expression of miR-212-3p and inhibited the expression of ATF2 in a dose-dependent manner. Low expression of miR-212-3p reversed the inhibitory effect of 400 μmol/L GPF on the proliferation and migration of HUVECs induced by IL-8. miR-212-3p targeted ATF2 and regulated its expression. Low expression of ATF2 can partially reversed the effect of low expres- sion of miR-212-3p on proliferation and migration of HUVECs induced by IL-8 treated with GPF.Conclusion GPF inhibits the prolif- eration and migration of HUVECs induced by IL-8 through miR-212-3p/ATF2 pathway. |
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