| 李杰玉,林玉玲,李曾一,等.长链非编码RNA DLX6-AS1 与微小RNA-346在糖尿病足72 例血清中的表达及其临床意义[J].安徽医药,2022,26(9):1858-1861. |
| 长链非编码RNA DLX6-AS1 与微小RNA-346在糖尿病足72 例血清中的表达及其临床意义 |
| Expression and clinical significance of the long noncoding RNA DLX6-AS1 and microR-346 in the serum of 72 cases of diabetic foot |
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| DOI：10.3969/j.issn.1009-6469.2022.09.038 |
| 中文关键词: 糖尿病足 糖尿病，2型 长链非编码RNA DLX6-AS1 微小RNA-346 炎性因子 |
| 英文关键词: Diabetic foot Diabetes mellitus, type 2 Long noncoding RNA DLX6-AS1 MicroR-346 Inflammatory factors |
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| 中文摘要: |
| 目的探讨长链非编码RNA(lncRNA)DLX6-AS1与微小RNA(miR)-346在糖尿病足病人血清中的表达及其临床意义。方法收集2017年5月至2018年12月南阳市中心医院收治的72例糖尿病足病人为糖尿病足组，选取同期该院收治的60例2型糖尿病无糖尿病足病人为糖尿病组，取同期于该院进行健康体检的健康志愿者55例作为对照组。实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测各组受检者血清中DLX6-AS1、miR-346的表达水平；采用酶联免疫吸附测定(ELISA)检测血管细胞黏附分子-1(VCAM-1)、成纤维细胞生长因子2(FGF2)、肿瘤坏死因子-α(TNF-α)水平；采用电化学发光法检测血清中白细胞介素-6(IL-6)水平；采用Pearson相关性分析检验糖尿病足病人血清DLX6-AS1、miR-346的表达水平与炎性因子的相关性；采用logistic回归分析研究影响糖尿病足发生的相关因素。结果糖尿病组、糖尿病足组病人VCAM-1、FGF2、TNF-α、IL-6水平高于对照组(P<0.05)，糖尿病组与糖尿病足组比较差异有统计学意义(P<0.05)；对照组DLX6-AS1的表达量是1.01±0.13，低于糖尿病组、糖尿病足组DLX6-AS1的表达量2.16±0.25、3.20±1.03(P<0.05)；对照组miR-346的表达水平是1.00±0.16，高于糖尿病组、糖尿病足组miR-346的表达水平0.73±0.11、0.41±0.08(P<0.05)，糖尿病组与糖尿病足组相比差异有统计学意义(P<0.05)；随着糖尿病足病人Wagner分级增加DLX6-AS1的表达水平显著升高(P<0.05)，miR-346的表达水平显著降低(P<0.05)；DLX6-AS1的表达与FGF2、TNF-α、IL-6呈正相关(P<0.05)，而miR-346与之呈负相关(P<0.05)；DLX6-AS1与miR-346表达量是影响糖尿病足发生的危险因素(P<0.05)。结论糖尿病足病人血清中DLX6-AS1表达量升高，而miR-346表达量降低，二者可能参与糖尿病足发生及发展过程，并可能作为临床诊断的分子标志物。 |
| 英文摘要: |
| Objective To investigate the expression and clinical significance of the long noncoding RNA DLX6-AS1 and microR-346 in the serum of diabetic foot patients.Methods Seventy-two patients with diabetic foot admitted to Nanyang Central Hospital from May 2017 to December 2018 were collected as the diabetic foot group, 60 patients with type 2 diabetes without diabetic foot who were admitted to the hospital during the same period were selected as the diabetic group, and those admitted to the hospital during the same period were selected as the diabetic foot group. Fifty-five healthy volunteers who underwent physical examination were used as the control group. The expression levels of DLX6-AS1 and miR-346 in the serum of the subjects in each group were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR). Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of vascular cell adhesion molecule-1 (VCAM-1), fibroblast growth factor 2 (FGF2) and tumor necrosis factor-α (TNF-α). Electrochemiluminescence was used to detect the level of interleukin-6 (IL-6) in serum. Pearson correlation analysis was used to test the correlation between the expression levels of DLX6-AS1 and miR-346 and inflammatory factors in diabetic foot patients. Logistic regression analysis was used to study the related factors affecting the occurrence of diabetic foot.Results The levels of VCAM-1, FGF2, TNF-α, and IL-6 in the diabetes group and diabetic foot group were higher than those in the control group (P < 0.05), and the difference between the diabetes group and the diabetic foot group was statistically significant (P < 0.05). The expression level of DLX6-AS1 in the control group was 1.01±0.13, which was lower than that in the diabetes group and diabetic foot group by 2.16±0.25 and 3.20±1.03, respectively (P < 0.05). The expression level of miR-346 in the control group was 1.00±0.16, which was higher than that in the diabetes group and the diabetic foot group by 0.73±0.11 and 0.41±0.08, respectively (P < 0.05), and the difference was significant between the diabetes group and the diabetic foot group (P < 0.05). With the increase in Wagner grade of diabetic foot patients, the expression level of DLX6-AS1 was significantly increased (P < 0.05), and the expression level of miR-346 was significantly decreased (P < 0.05). The expression of DLX6-AS1 was positively correlated with FGF2, TNF-α, and IL-6 (P < 0.05), while miR-346 was negatively correlated with it (P < 0.05). The expression levels of DLX6-AS1 and miR-346 were related to diabetes risk factors for foot development (P < 0.05).Conclusions The expression of DLX6-AS1 in the serum of diabetic foot patients was increased, while the expression of miR-346 was decreased. Both may be involved in the occurrence and development of diabetic foot and may be used as molecular markers for clinical diagnosis. |
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