| 芦君.三七总皂苷对脑梗死大鼠神经细胞 Wnt/β-连环素信号通路的影响[J].安徽医药,2022,26(12):2395-2398. |
| 三七总皂苷对脑梗死大鼠神经细胞 Wnt/β-连环素信号通路的影响 |
| Effects of panax notoginseng saponins on neuron Wnt/β-catenin signaling pathway in rats with cerebral infarction |
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| DOI：10.3969/j.issn.1009-6469.2022.12.013 |
| 中文关键词: 脑梗死 人参皂甙类 三七 β连环素 Wnt信号通路 神经细胞 自噬 大鼠， Sprague-Dawley |
| 英文关键词: Cerebral infarction Ginsenosides Panax notoginseng Beta Catenin Wnt signaling pathway Neuron Autopha. gy Rats,Sprague-Dawley |
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| 中文摘要: |
| 目的探究三七总皂苷对脑梗死大鼠神经细胞 Wnt/β-连环素(β-catenin)信号通路的影响。方法该研究起止时间为 2018年1月至 2019年3月。 60只雄性 SD大鼠采用随机数字表法分为空白对照组、模型组、低剂量三七总皂苷组(75 mg/kg)和高剂量三七总皂苷组(150 mg/kg)，各 15只。将模型组、低剂量三七总皂苷组和高剂量三七总皂苷组大鼠制成脑梗死模型；低剂量、高剂量三七总皂苷组均腹腔注射三七总皂苷，空白对照组和模型组注射等量生理盐水，每日 1次，注射 3d。使用改良神经功能缺损评分(mNSS)评价大鼠神经功能；使用 Z-Longa评分评价大鼠姿势反射情况；检测各组大鼠脑梗死面积及脑含水率；使用蛋白质印迹法检测 Wnt/ β-catenin信号通路蛋白及自噬相关蛋白表达情况。结果与空白对照组相比，模型组大鼠 mNSS评分、大鼠姿势反射评分、脑梗死率、脑含水率、微管相关蛋白轻链 3-Ⅱ(LC3-Ⅱ)蛋白表达水平升高(P<0.05)自噬蛋白 P62表达、 Wnt信号通路配体蛋白 Wnt3a蛋白表达(0.25±0.09比 1.68±0.25)和 β-catenin蛋白表达(0.40±0.10比 1.55±0.20)，降低(P<0.05)。与模型组相比，低、高剂量三七总皂苷组大鼠 mNSS评分、大鼠姿势反射评分、脑梗死率、脑含水率降低， LC3-Ⅱ蛋白表达降低(P<0.05)P62蛋白、 Wnt3a蛋白(0.79±0.15，1.26±0.25比0.25±0.09)和β-catenin蛋白表达(1.23±0.25，0.90±0.15比0.40±0.10)升高(P<0.05)低剂量三七总皂苷组相比，高剂。与，量三七总皂苷组大鼠 mNSS评分、大鼠姿势反射评分、脑梗死率、脑含水率降低， LC3-Ⅱ蛋白表达降低(P<0.05)P62蛋白、 Wnt3a蛋白(1.26±0.25比 0.79±0.15)和 β-catenin蛋白表达( 1.23±0.25比 0.90±0.15)升高( P<0.05)。结论三七总皂可以，通过促进 Wnt/β-catenin信号通路蛋白表达激活 Wnt/β-catenin信号通路，发挥调节大鼠神经元细胞的自噬，改善脑梗死大鼠神经损伤的作用。 |
| 英文摘要: |
| Objective To explore the effects of panax notoginseng saponins (PNS) on neuron Wnt/β-catenin signaling pathway in rats with cerebral infarction.Methods The study period was from January 2018 to March 2019. Sixty male SD rats were divided into blank control group, model group, low-dose PNS group (75 mg/kg) and high-dose PNS group (150 mg/kg) using random number table method, with 15 cases in each group. Rats in model group, low-dose PNS group and high-dose PNS group were made into cerebral infarction mod.els. The administration groups were intraperitoneally injected with PNS, while blank control group and model group were injected with thesame volume of normal saline, once/d for 3 d. The nerve function of rats was evaluated by modified neurological severity score points(mNSS). The posture reflex was evaluated by Z-Longa scores. The cerebral infarct area and cerebral water rate in each group were detect. ed. The expressions of Wnt/β-catenin signaling pathway proteins and autophagy-related proteins were detected by Western blotting.Re. sults Compared with blank control group,mNSS score of posture reflex, cerebral infarction rate, brain water rate, expression level of mi.crotubule-associated protein 1 light chain 3 (LC3-Ⅱ) protein were increased in model group (P<0.05), and expression levels of autophagy protein P62, Wnt signaling pathway ligand Wnt3a protein (0.25±0.09 vs. 1.68±0.25) and β-catenin protein (0.40 ±0.10 vs. 1.55 ±0.20) were decreased (P<0.05). Compared with model group, mNSS, score of posture reflex, cerebral infarction rate, brain water rate and expres. sion of LC3-Ⅱ protein were decreased in low-dose and high-dose PNS group(P<0.05), and expressions of P62 protein, Wnt3a protein (0.79 ± 0.15), (1.26 ± 0.25 vs. 0.25 ± 0.09) and β-catenin protein (1.23 ± 0.25, 0.90 ± 0.15 vs. 0.40 ± 0.10) were increased (P<0.05). Com. pared with low-dose PNS group, mNSS, score of posture reflex, cerebral infarction rate and brain water rate were decreased in high-dose PNS group, expression of LC3-Ⅱ protein was decreased (P<0.05), and expressions of P62 protein, Wnt3a protein (1.26±0.25 vs. 0.79± 0.15) and β-catenin protein (1.23±0.25 vs. 0.90±0.15)were increased (P<0.05).Conclusion PNS can activate Wnt/β-catenin signaling pathway by promoting expressions of Wnt/β-catenin signaling pathway proteins, which can regulate neuron autophagy and improve nerve damage in rats with cerebralinfarction. |
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