| 魏晓霞,周艳彩,卓亚,等.慢性乙型肝炎病人血清缺氧诱导因子 1α水平及其诊断肝纤维化的价值[J].安徽医药,2022,26(12):2439-2442. |
| 慢性乙型肝炎病人血清缺氧诱导因子 1α水平及其诊断肝纤维化的价值 |
| Serum hypoxia-inducible factor 1α in patients with chronic hepatitis B and its diagnostic value for hepatic fibrosis |
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| DOI：10.3969/j.issn.1009-6469.2022.12.023 |
| 中文关键词: 乙型肝炎，慢性 前胶原 层粘连蛋白 透明质酸 肝纤维化 缺氧诱导因子 1α |
| 英文关键词: Hepatitis B, chronic Procollagen Laminin Hyaluronic acid Liver fibrosis Hypoxia-inducible factor 1α |
| 基金项目:河南省医学科技攻关计划项目( 201303107) |
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| 中文摘要: |
| 目的探讨慢性乙型肝炎( CHB)病人血清中缺氧诱导因子 1α(HIF-1α)水平及 HIF-1α在诊断 CHB病人发生肝纤维化中的价值。方法纳入 2017年 6月至 2019年 1月于新乡医学院第一附属医院住院且行肝脏穿刺活检的 80例 CHB病人和 30例健康体检者(对照组)作为研究对象。 CHB病人根据肝脏纤维化分期分为 20例无纤维化组( S0)、 39例轻度纤维化组(S1~S2)和 21例中重度纤维化组( S3~S4)。检测并比较 CHB病人和对照组血清 Ⅲ型前胶原( PCⅢ)、 Ⅳ型胶原、透明质酸、层粘连蛋白(LN)、 HIF-1α水平的差异， CHB病人血清 HIF-1α水平与 PCⅢ、Ⅳ型胶原、透明质酸和 LN相关性采用 Pearson相关分析，利用受试者操作特征曲线( ROC曲线)探讨 PCⅢ、Ⅳ型胶原、透明质酸、 LN和 HIF-1α在诊断 CHB病人发生肝纤维化临床效能的差异。结果 S0组、 S1~S2组和 S3~S4组病人血清 PCⅢ、Ⅳ型胶原、透明质酸、 LN及 HIF-1α［( 46.37±9.15)μg/L、(60.31±10.02)μg/L、(89.39±12.07)μg/L比( 35.69±7.25)μg/L］表达水平较对照组升高( P<0.05)。 S3~S4组病人血清 PCⅢ、Ⅳ型胶原、透明质酸、 LN和 HIF-1α水平均显著高于 S1~S2组和 S0组( P<0.05)。 S1~S2组病人血清 PCⅢ、透明质酸、 LN及 HIF-1α水平均显著高于 S0组( P<0.05)。 CHB病人血清 HIF-1α水平与 PCⅢ、Ⅳ型胶原、透明质酸和 LN均呈现显著正相关性( P<0.05)相关系数分别 |
| 英文摘要: |
| Objective To investigate the serum level of hypoxia-inducible factor 1α (HIF-1α) in patients with chronic hepatitis B (CHB) and its value in diagnosing the development of liver fibrosis.Methods A total of 80 patients with CHB who were hospitalized atthe First Affiliated Hospital of Xinxiang Medical University from June 2017 to January 2019 and underwent liver puncture biopsy and30 healthy physical examiners (control group) were included as study subjects. According to the liver fibrosis stage, CHB patients weredivided into 20 nonfibrosis group (S0), 39 mild fibrosis group (S1-S2) and 21 moderate-to-severe fibrosis group (S3-S4). The differences in serum levels of procollagen Ⅲ (PCⅢ), type Ⅳ collagen, hyaluronic acid, laminin (LN) and HIF-1α between CHB patients and con. trols were detected and compared, and the correlation between serum HIF-1α levels and PCⅢ, type Ⅳ collagen, hyaluronic acid, andLN in CHB patients was performed using Pearson correlation analysis. The differences in the clinical efficacy of PCⅢ, type Ⅳ colla.gen, hyaluronic acid, LN and HIF-1α in diagnosing the development of liver fibrosis in patients with CHB were explored using receiveroperating characteristic (ROC) curves.Results Compared with the control group, the serum expression levels of PCⅢ, type Ⅳ colla. gen, hyaluronic acid, LN and HIF-1α in patients in groups S0, S1-S2 and S3-S4 [(46.37±9.15) μg/L, (60.31±10.02) μg/L, (89.39± 12.07) μg/L vs. (35.69±7.25) μg/L] were significantly elevated (P<0.05). Serum levels of PCⅢ, type Ⅳ collagen, hyaluronic acid, LN and HIF-1α in group S3-S4 were significantly higher than those in groups S1-S2 and S0 (P<0.05). Serum levels of PCⅢ, hyaluronic ac. id, LN and HIF-1α in the S1-S2 group were significantly higher than those in the S0 group (P<0.05). The serum HIF-1α levels in CHB patients showed significant positive correlations (P<0.05) with PCIII, type Ⅳ collagen, hyaluronic acid and LN, with correlation coeffi. cients of 0.54, 0.25, 0.42 and 0.13, respectively. The AUCs for hepatic fibrosis in patients with CHB diagnosed with HIF-1α, PCⅢ,type Ⅳ collagen, hyaluronic acid and LN were 0.947, 0.778, 0.733, 0.874 and 0.856, respectively. The AUC for the occurrence of liverfibrosis was significantly higher (P<0.05). When the cutoff value of HIF-1α was 71.26 μg/L, the sensitivity and specificity were 92.36% and 95.26%, respectively.Conclusion Serum levels of HIF-1α in CHB patients were significantly higher, HIF-1α may be involved in the pathogenesis of liver fibrosis in CHB patients, and HIF-1α can be used as a serological marker for the diagnosis of liver fibrosis. |
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