| 张文博,郭宇,袁园,等.2型糖尿病病人外周血同源性磷酸酶 -张力蛋白诱导的激酶 1、 Parkin蛋白、视神经蛋白表达与肾损伤程度的关系研究[J].安徽医药,2022,26(12):2479-2484. |
| 2型糖尿病病人外周血同源性磷酸酶 -张力蛋白诱导的激酶 1、 Parkin蛋白、视神经蛋白表达与肾损伤程度的关系研究 |
| Study on relationship between expressions of peripheral blood phosphatase and tensin homologue-induced putative kinase 1, Parkin protein and optineurin and degree of renal injury in patients with type 2 diabetes mellitus |
| |
| DOI：10.3969/j.issn.1009-6469.2022.12.032 |
| 中文关键词: 糖尿病肾病 线粒体自噬 同源性磷酸酶 -张力蛋白诱导的激酶 1 Parkin蛋白 视神经蛋白 |
| 英文关键词: Diabetic nephropathy Mitophagy Phosphatase and tensin homologue-induced putative kinase 1 Parkin protein Optineurin |
| 基金项目:河南省医学科技攻关计划联合共建项目( LHGJ20190550) |
|
| 摘要点击次数: 745 |
| 全文下载次数: 281 |
| 中文摘要: |
| 目的探究 2型糖尿病( T2DM)病人外周血同源性磷酸酶 -张力蛋白诱导的激酶 1(PINK1)、 Parkin蛋白、视神经蛋白(OPTN)表达及与肾损伤程度关系。方法选取 2019年 5月至 2021年 5月河南科技大学第一附属医院 156例 T2DM病人，根据糖尿病肾病( DN)诊断分期标准，分为非 DN组 92例、早期 DN组( EDN组) 38例、临床期 DN组( CDN组) 26例，另以同期 60例健康体检者为健康组。比较四组病人外周血 PINK1、Parkin蛋白、 OPTN表达量(荧光定量 PCR法)；采用 Pearson相关分析法探究 PINK1、Parkin蛋白、 OPTN表达量与糖脂代谢空腹血糖、糖化血红蛋白( HbA1c)、总胆固醇、三酰甘油、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇( HDL-C)及肾功能指标血尿素氮、血肌酐、预估肾小球滤过率( eGFR)、尿微量白蛋白 /尿肌酐(UACR)、 24 h尿蛋白排泄率( UAER)相关性，采用多因素 logistic回归分析探究 DN发生影响因素，绘制 ROC曲线分析 PINK1、 Parkin蛋白、 OPTN表达量对 DN诊断价值。结果 CDN组、 EDN组、非 DN组、健康组外周血 PINK1 mRNA表达量分别为 0.31±0.17、0.44±0.20、0.69±0.35、0.73±0.34，Parkin蛋白 mRNA分别为 0.51±0.12、0.62±0.18、0.79±0.28、0.98±0.35，OPTN mRNA分别为 0.05±0.01、0.10±0.03、0.14±0.05、0.18±0.07，四组病人外周血 PINK1、Parkin蛋白、 OPTN表达量差异有统计学意义( P<0.05)，均表现为 CDN组 |
| 英文摘要: |
| Objective To investigate the expressions of phosphatase and tensin homologue-induced putative kinase 1 (PINK1), Par.kin protein and optineurin (OPTN) in peripheral blood of patients with type 2 diabetes mellitus (T2DM) and their relationship with thedegree of renal injury.Methods A total of 156 patients with T2DM in The First Affiliated Hospital of Henan University of Science andTechnology between May 2019 and May 2021 were selected, and divided into non-DN group (92 cases), early DN group (EDN group,38 cases) and clinical DN group (CDN group, 26 cases) according to the diagnostic staging criteria of diabetic nephropathy (DN). Anoth.er 60 healthy people with physical examination during the same period were selected as control group. Expression levels of peripheralblood PINK1, Parkin and OPTN were compared among the four groups (fluorescence quantitative PCR). Pearson correlation analysiswas applied to explore the correlation between expression levels of PINK1, Parkin and OPTN and glucolipid metabolism [fasting bloodglucose, glycosylated hemoglobin (HbA1c), total cholesterol, triglyceride, low density lipoprotein cholesterol (LDL-C), high density lipo. protein cholesterol (HDL-C)] and renal function indicators [blood urea nitrogen, serum creatinine, estimated glomerular filtration rate(eGFR), urinary albumin creatinine ratio (UACR), 24 h urinary albumin ejection rate (UAER)]. Multivariate logistic regression analysiswas adopted to explore the influencing factors of DN occurrence, and ROC curve was drawn to analyze the diagnostic value of expres.sion levels of PINK1, Parkin and OPTN on DN.Results The expression levels of peripheral blood PINK1 mRNA in CDN group, EDN group, non-DN group and control group were 0.31±0.17, 0.44±0.20, 0.69±0.35 and 0.73±0.34, and the expression levels of ParkinmRNA were 0.51±0.12, 0.62±0.18, 0.79±0.28 and 0.98±0.35, and the expression levels of OPTN mRNA were 0.05±0.01, 0.10±0.03,0.14±0.05 and 0.18±0.07, respectively. There were statistical differences among the four groups in terms of expression levels of periph.eral blood PINK1, Parkin and OPTN (P<0.05), and the above expression levels were shown as CDN group < EDN group < non-DN group < control group (P<0.05). The expression levels of peripheral blood PINK1 and Parkin of patients with T2DM were negatively cor.related with fasting blood glucose, HbA1c, blood urea nitrogen, serum creatinine, UACR and UAER (P<0.05), and were positively cor. related with eGFR (P<0.05). OPTN was negatively correlated with blood urea nitrogen, serum creatinine, UACR and UAER (P<0.05), and was positively correlated with eGFR (P<0.05). The course of diabetes mellitus, HbA1c, UACR and UAER were independent risk factors for DN (P<0.05), and PINK1, Parkin and OPTN were protective factors (P<0.05). The areas under the ROC curves of expressionlevels of peripheral blood PINK1, Parkin and OPTN in the diagnosis of DN were 0.709, 0.754 and 0.839, respectively.Conclusions The expression levels of PINK1, Parkin and OPTN in peripheral blood of patients with T2DM are all down-regulated, and decrease withthe increase of the degree of renal injury. Detection of expression levels of peripheral blood PINK1, Parkin and OPTN has certain diag.nostic value on DN. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
