| 吴春燕,汪晓燕,李炎.微 RNA-124-3p在溃疡性结肠炎病人外周血中的表达及意义[J].安徽医药,2024,28(1):168-172. |
| 微 RNA-124-3p在溃疡性结肠炎病人外周血中的表达及意义 |
| Expression and significance of miR-124-3p in the peripheral blood of patients with ulcerative colitis |
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| DOI：10.3969/j.issn.1009-6469.2024.01.035 |
| 中文关键词: 结肠炎，溃疡性 C反应蛋白质 微 RNA-124-3p 外周血 病情严重程度 |
| 英文关键词: Colitis,ulcerative C-reactive protein MicroRNA-124-3p Peripheral blood Severity of illness |
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| 中文摘要: |
| 目的检测溃疡性结肠炎( UC)病人外周血中微 RNA-124-3p(miR-124-3p)的表达水平，并讨论其临床意义。方法选择 121例 UC病人( UC组)和 60例健康体检者(对照组)作为研究对象进行前瞻性研究， UC组来源于 2019年 8月至 2021年 8月期间武汉市蔡甸区人民医院住院治疗的病人，对照组为同期来院进行健康体检的个体。采用实时荧光定量 PCR(qRT-PCR)检测 miR-124-3p在各组外周血中的表达水平，酶联免疫吸附实验( ELISA)法检测 C-反应蛋白( CRP)在各组外周血中的表达水平。 UC活动期病人外周血 miR-124-3p表达水平与 CRP水平的相关性采用 Pearson法，与 UC内镜严重指数( UCEIS)评分、改良梅奥( Mayo)评分的相关性分析采用 Spearman法。结果与对照组相比， UC组病人外周血中 miR-124-3p的表达水平显著降低(0.84±0.22比 0.52±0.17，P<0.05)CRP水平显著升高［( 7.08±2.52)mg/L比( 9.83±2.91)mg/L，P<0.05］。与 UC缓解期病人相比，活动期病人外周血中 miR-124-3p的，表达水平显著降低( 0.67±0.22比 0.44±0.14，P<0.05)，CRP水平显著升高［(9.06±2.85)mg/L比( 10.25±2.94)mg/L，P<0.05］。 UC不同临床活动度病人外周血 miR-124-3p表达水平由高至低依次为轻度( 0.53±0.16)、中度(0.45±0.13)、重度( 0.30±0.09)，CRP表达水平及 UCEIS评分、改良 Mayo评分由高至低依次为重度［( 12.03±3.02)mg/L、(5.97±1.82)分、(11.53±0.46)分］、中度［(10.35±2.79)mg/L、(4.82±1.51)分、(7.70±1.64)分)］、轻度［( 8.74±2.83)mg/L、(3.03±0.90)分、(3.98±0.95)分］组间比较差异有统计学意义( P<0.05)。 UC活动期病人外周血 miR-124-3p表达水平与 CRP、UCEIS评分及改良 Mayo评分均呈负，相关( r=-0.57、-0.44、-0.59，P<0.05)。结论 UC病人外周血 miR-124-3p表达水平降低，其水平变化与 UC疾病活动和病情程度密切相关。 |
| 英文摘要: |
| Objective To detect the expression level of microRNA-124-3p (miR-124-3p) in the peripheral blood of patients with ul- cerative colitis (UC) and to discuss its clinical significance. Methods A prospective study was conducted on 121 UC patients (UCgroup) and 60 healthy subjects (control group). The UC group included patients hospitalized in Wuhan Caidian District People's Hospi-tal from August 2019 to August 2021, and the control group was from individuals who came to the hospital for physical examination dur-ing the same period. Real-time quantitative PCR (qRT-PCR) was used to detect the expression level of miR-124-3p in peripheral blood of each group, and enzyme linked immunosorbent assay (ELISA) was used to detect the expression level of C-reactive protein (CRP) inperipheral blood of each group. Pearson method was used to analyze the correlation between the expression level of miR-124-3p in pe-ripheral blood and CRP level in patients with active UC, and Spearman method was used to analyze the correlation with ulcerative coli-tis endoscopic indexof severity (UCEIS) score and modified Mayo score.Results Compared with the control group, the expression lev- el of miR-124-3p in peripheral blood of patients in UC group was significantly decreased [0.84±0.22, (0.52±0.17)mg/L, P<0.05], and the CRP level was significantly increased [(7.08±2.52) mg/L vs. (9.83±2.91) mg/L, P<0.05]. Compared with UC patients in remission stage, the expression level of miR-124-3p in peripheral blood of UC patients in active stage was significantly decreased [0.67±0.22, (0.44±0.14)mg/L, P<0.05]. CRP level was significantly increased [(9.06±2.85) mg/L vs. (10.25±2.94) mg/L, P<0.05)]. The expression levels of miR-124-3p in peripheral blood of patients with different clinical activities of UC were mild (0.53±0.16), moderate (0.45±0.13), and severe (0.30±0.09) from high to low, respectively. CRP expression level, UCEIS score and modified Mayo score were severe[(12.03±3.02) mg/L, (5.97±1.82) points, (11.53±0.46) points], moderate [(10.35±2.79) mg/L, (4.82±1.51) points, (7.70±1.64) points)],mild [(8.74±2.83) mg/L, (3.03±0.90) points, (3.98±0.95) points] from high to low, and the difference between groups was statistically sig-nificant (P<0.05). The expression level of miR-124-3p in peripheral blood of patients with UC activity was negatively correlated with CRP, UCEIS score and modified Mayo score (r=-0.57, -0.44, -0.59, P<0.05).Conclusion The expression level of miR-124-3p in pe- ripheral blood of UC patients decreased, and its change was closely related to the disease activity and severity of UC. |
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