| 孙玉,南照林,张彦敏,等.血清半乳糖凝聚素 -3、α-klotho表达与代谢相关脂肪性肝病糖脂水平及预后的关系[J].安徽医药,2024,28(2):357-361. |
| 血清半乳糖凝聚素 -3、α-klotho表达与代谢相关脂肪性肝病糖脂水平及预后的关系 |
| Correlation of serum Gal-3 and α-klotho expressions with glucose and lipid levels and prognosis in patients with metabolic-related fatty liver disease |
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| DOI：10.3969/j.issn.1009-6469.2024.02.032 |
| 中文关键词: 代谢相关脂肪性肝病 非酒精性脂肪性肝病 α-klotho 半乳糖凝聚素 -3 糖脂水平 预后 |
| 英文关键词: Metabolism-associated fatty liver disease Non-alcoholic fatty liver disease α -klotho Galectin-3 Glycolipid level Prognosis |
| 基金项目:保定市科技计划项目( 2141ZF286) |
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| 中文摘要: |
| 目的探讨半乳糖凝聚素 -3(Gal-3)、 α-klotho在代谢相关脂肪性肝病( MAFLD)病人血清中的表达情况，并分析其与糖脂水平和预后的关系。方法选取 2020年 12月至 2021年 12月来保定市人民医院就诊并确诊为 MAFLD病人 112例为研究对象，根据非酒精性脂肪性肝病肝纤维化评分( NAFLDFS)将病人分为肝纤维化危险组( n=46)、无肝纤维化组( n=66)同时选取 50例健康者为对照组。收集研究对象一般资料，采用酶联免疫吸附法对血清中 α-klotho、Gal-3表达水平进行检测，P，earson法分析 α-klotho、Gal-3表达的相关性，及两者分别与糖脂代谢指标的相关性；受试者操作特征(ROC)曲线分析血清 α-klotho、Gal-3水平对 MAFLD病人预后的预测价值。结果 MAFLD病人血清中 α-klotho表达水平低于对照组( 268.15±82.13)ng/L，Gal-3表达水平高于对照组( 1.48±0.47)μg/L，其中肝纤维化危险组病人血清中 α-klotho表达水平低于无肝纤维化组［( 140.49±45.16) ng/L比( 215.51±70.33)ng/L］Gal-3表达水平高于无肝纤维化组［( 2.26±0.50)μg/L比( 1.96±0.33)μg/L］(P<0.05)；预后不良的 MAFLD病人血清中 α-klotho表，达水平低于预后良好病人， Gal-3高于预后良好病人，均差异有统计学意义( P<0.05)。 Pearson法分析结果显示， MAFLD病人血清中 α-klotho、Gal-3表达呈显著负相关( r=.0.49，P<0.05)； MAFLD病人血清中 α-klotho表达与空腹血糖( FBG)、空腹胰岛素( FINS)、胰岛素抵抗( HOMA-IR)、总胆固醇( TC)、三酰甘油( TG)、低密度脂蛋白胆固醇( LDL-c)、天冬氨酸转氨酶( AST)、谷丙转氨酶( ALT)、谷氨酰转肽酶( GGT)表达呈负相关，与高密度脂蛋白胆固醇( HDL-c)、血小板(PLT)、血清白蛋白( Alb)表达呈正相关( P<0.05)； Gal-3表达与 FBG、FINS、HOMA-IR、TC、TG、LDL-c、AST、ALT、GGT表达呈正相关，与 HDL-c、PLT、Alb表达呈负相关(P<0.05)。 ROC曲线结果显示，血清 α-klotho、Gal-3水平评估预后不良病人的曲线下面积( AUC)分别为 0.83、0.84，对应的灵敏度分别为 86.84%、80.90%，特异度分别为 66.22%、82.02%；二者联合评估的 AUC为 0.93，灵敏度为 86.84%，特异度为 89.19%。结论 MAFLD病人血清中 α-klotho表达降低， Gal-3表达升高，二者表达与糖脂水平和病人预后密切相关。 |
| 英文摘要: |
| Objective To investigate the expressions of galectin-3 (Gal-3) and α-klotho in serum of patients with metabolically-asso. ciated fatty liver disease (MAFLD), and to analyze their relationship with glycolipid levels and prognosis.Methods A total of 112 pa.tients who came to the People's Hospital of Baoding and were diagnosed with MAFLD from December 2020 to December 2021 werecollected as the research objects. According to the non-alcoholic fatty liver disease liver fibrosis score (NAFLDFS), the patients were grouped into liver fibrosis risk group (n=46 cases) and no liver fibrosis group (n=66 cases), and 50 healthy subjects were regarded ascontrol group. The general information about the research subjects were collected, the expression levels of α-klotho and Gal-3 in serum were detected by enzyme-linked immunosorbent assay, Pearson method was used to analyze the correlation of α-klotho and Gal-3 ex. pression, and their correlation with glucose and lipid metabolism indexes, respectively; receiver operating characteristic (ROC) curvewas used to analyze the predictive value of serum α-klotho and Gal-3 levels in the prognosis of MAFLD patients.Results The expres. sion level of α-klotho in serum of MAFLD patients was lower than that of the control group(268.15±82.13)ng/L, and the expression level of Gal-3 was higher than that of the control group(1.48±0.47)μg/L, among them, the expression level of α-klotho in serum of patients with hepatic fibrosis risk group was lower than that of non-hepatic fibrosis group[(140.49±45.16)ng/L vs. (215.51±70.33)ng/L], and the expression level of Gal-3 was higher than that in the non-hepatic fibrosis group[(2.26±0.50)μg/L vs. (1.96±0.33)μg/L] (P<0.05); the ex. pression level of α-klotho in serum of MAFLD patients with poor prognosis was lower than that of patients with good prognosis, and Gal-3 was higher than that of patients with good prognosis (P<0.05). The results of Pearson's method analysis showed that the expressions of α-klotho and Gal-3 in serum of MAFLD patients were greatly negatively correlated (r=.0.49, P<0.05); the expression of α -Klotho was negatively correlated with the expression of FBG, FINS, HOMA-IR, TC, TG, LDL-C, AST, ALT and GGT, but positively correlated with the expression of HDL-C,PLT and Alb in serum of MAFLD patients (P<0.05). Gal-3 expression was positively correlated with FBG, FINS,HOMA-IR,TC,TG,LDL-C,AST,ALT and GGT,and negatively correlated with HDL-C, PLT and Alb (P<0.05). The results of the ROC curve showed that the area under the curve (AUC) of serum α-klotho and Gal-3 levels in evaluating patients with poor prognosiswas 0.83 and 0.84,respectively, with corresponding sensitivity of 86.84% and 80.90%,and specificity of 66.22% and 82.02%,respec.tively; the AUC of the combined assessment was 0.930, the sensitivity was 86.84%, and the specificity was 89.19%.Conclusion The expression of α-klotho in serum of MAFLD patients is decreased, and the expression of Gal-3 is increased, and the expressions of the two are closely related to the level of glycolipid and the prognosis of patients. |
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