| 许国磊,吴宝,吴欣芳,等.血清长链非编码 RNA肌动蛋白纤维相关蛋白 1-反义 RNA1水平与钙化性主动脉瓣狭窄病人左心室功能的相关性研究[J].安徽医药,2024,28(3):542-547. |
| 血清长链非编码 RNA肌动蛋白纤维相关蛋白 1-反义 RNA1水平与钙化性主动脉瓣狭窄病人左心室功能的相关性研究 |
| Correlation between serum lncRNA AFAP1-AS1 level and left ventricular function in patients with aortic valve stenosis |
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| DOI：10.3969/j.issn.1009-6469.2024.03.025 |
| 中文关键词: 主动脉瓣狭窄 肌动蛋白纤维相关蛋白 1-反义 RNA1 钙质沉着症 左心室功能 严重程度 相关性 )、严重， |
| 英文关键词: Aortic valve stenosis Actin filament-associated protein 1-antisense RNA 1 Calcinosis Left ventricular function |
| 基金项目:中国中医科学院广安门医院南区院级科研基金课题( Y2021-06) |
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| 中文摘要: |
| 目的分析血清长链非编码 RNA(lncRNA)肌动蛋白纤维相关蛋白 1-反义 RNA1(AFAP1-AS1)表达水平与钙化性主动脉瓣狭窄( CAS)病人左心室收缩及舒张功能的相关性。方法于 2020年 1月至 2021年 12月，选取中国中医科学院广安门医院就诊的 CAS病人 129例作为 CAS组［左心室射血分数( LVEF)≥50%］同期该院健康志愿者 130例作为对照组。收集病人人口学资料、超声及实验室生化指标，检测血清 lncRNA AFAP1-AS1表达。，受试者操作特征曲线( ROC曲线)分析血清 lncRNA AFAP1-AS1诊断 CAS效能。结果对照组血清 lncRNA AFAP1-AS1表达水平( 1.15±0.18)低于 CAS组( 1.58±0.30)(P<0.001)。轻度狭窄者血清 lncRNA AFAP1-AS1表达水平( 1.37±0.26)低于中、重度狭窄者，而中度狭窄者 lncRNA AFAP1-AS1表达水平(1.59±0.30)低于重度狭窄者( 1.79±0.34)(P<0.001)。 ROC结果显示，血清 lncRNA AFAP1-AS1诊断 CAS、重度狭窄的曲线下面积分别为 0.86［95%CI:(0.82，0.91)］、 0.88［95%CI:(0.82，0.94)］。 CAS组 AVA水平低于对照组( P<0.001)左室舒张末期内径(LVEDD)、左室舒张末期容积( LVEDV)、室间隔厚度( IVST)、左室后壁厚度( LVPWT)、左房前后径( LAD)、，主动脉瓣平均压差(PGmean)、主动脉瓣峰值流速( Vmax)水平高于对照组(均 P<0.001)。相关性分析显示，血清 lncRNA AFAP1-AS1与 LVEDD、 |
| 英文摘要: |
| Objective To analyze the correlation between serum long non-coding RNA (lncRNA) actin filament-associated protein 1-antisense RNA 1 (AFAP1-AS1) expression level and left ventricular contraction and diastolic function in patients with calcific aortic stenosis (CAS).Methods One hundred and twenty-nine patients with calcified aortic stenosis (CAS) in Guang'anmen Hospital, Chi.nese Academy of traditional Chinese Medicine from January 2020 to December 2021 were taken as the CAS group (Left ventricularejection fraction≥50%), and 130 healthy volunteers were regarded as the control group. The demographic data, ultrasound and laborato.ry biochemical indicators of the patients were collected, the expression of serum lncRNA AFAP1-AS1 was detected; and the receiver operating characteristic curve (ROC) was used to analyze the efficacy of serum lncRNA AFAP1-AS1 in the diagnosis of CAS.Results The expression level of serum lncRNA AFAP1-AS1 in the control group (1.15±0.18) was lower than that in the CAS group (1.58±0.30) (P<0.001). The expression level of serum lncRNA AFAP1-AS1 in patients with mild stenosis (1.37±0.26) was lower than that in pa.tients with moderate and severe stenosis, while the expression level of lncRNA AFAP1-AS1 in patients with moderate stenosis (1.59± 0.30) was lower than that in patients with severe stenosis (1.79±0.34) (P<0.001). The ROC results showed that the area under the curve of serum lncRNA AFAP1-AS1 for the diagnosis of CAS and severe stenosis was 0.86 [95%CI: (0.82, 0.91)] and 0.88 [95%CI: (0.82, 0.94)], respectively. The level of aortic valve orifice area (AVA) in the CAS group was lower than that in the control group (P<0.001),and the levels of left ventricular end diastolic diameter (LVEDD), left ventricular end diastolic volume (LVEDV), interventricular septal thickness (IVST), left ventricular posterior wall thickness (LVPWT), left anterior posterior diameter, mean aortic valve pressure differ.ence (PGmean), and peak aortic valve velocity (Vmax) were obviously higher than those in the control group (all P<0.001). Correlation analysis showed that serum lncRNA AFAP1-AS1 was positively correlated with LVEDD, Vmax, peak early diastolic blood flow veloci.ty, peak late diastolic blood flow velocity, LVEDV, PGmean, and LVESD (r=0.60, 0.66, 0.72, 0.68, 0.56, 0.57, 0.50, all P<0.001), and negatively correlated with LVEF and AVA (r=.0.78, .0.62, all P<0.001).Conclusion The expression level of serum lncRNA AFAP1-AS1 in CAS patients is increased, which is related to the severity of CAS, the left ventricular diastolic and systolic functions, and canbe used as a non-invasive serum marker to assist clinical diagnosis of CAS. |
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