| 王博,王瑞,郭丽娜,等.基于网络药理学的双黄连抗炎作用机制分析[J].安徽医药,2021,25(3):435-440. |
| 基于网络药理学的双黄连抗炎作用机制分析 |
| Anti-inflammatory mechanism analysis of Shuanghuanglian based on network pharmacology |
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| DOI：10.3969/j.issn.1009-6469.2021.03.003 |
| 中文关键词: 中草药 药理作用 忍冬属 黄芩 连翘属 炎症 双黄连 网络药理学 |
| 英文关键词: Drugs, Chinese herbal Pharmacologic actions Lonicera Scutellaria baicalensis Forsythia Inflamma? tion Shuanghuanglian Network pharmacology |
| 基金项目:河南省中药制剂现代化技术研发与临床应用工程研究中心(豫发改高技〔2019〕569号) |
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| 中文摘要: |
| 目的探讨双黄连抗炎的主要活性成分和药理作用机制。方法通过中药系统药理学分析平台( TCMSP)检索“金银花”“黄芩”“连翘”三味药材的化学成分和作用靶点，构建化合物 -靶点网络；通过比较毒物基因组学数据库( Comparative Toxi? cogenomics Database，CTD)检索炎症相关基因，构建蛋白互作( protein-protein interaction，PPI)网络，进而在 DAVID平台进行基因本体( gene ontology，GO)功能富集分析和 KEGG通路富集分析，推测双黄连抗炎的作用机制。结果筛选得到与炎症有关的 36个化合物和 53个潜在靶点，关键靶点为前列腺素 G/H合成酶 2(Prostaglandin G/H synthase 2，PTGS2)、前列腺素 G/H合成酶 1(Prostaglandin G/H synthase 1，PTGS1)、诱导型一氧化氮合酶( Nitric oxide synthase，inducible，NOS2)、转录因子 p65(Tran? scription factor p65，RELA)、外消旋 -α丝氨酸 /苏氨酸蛋白激酶( RAC-alpha serine/threonine-protein kinase，AKT1)。 GO功能富集分析筛选得到 GO条目 77个， KEGG通路富集筛选得到 46条信号通路。结论初步预测了双黄连抗炎的药效成分和药理作用机制，为进一步深入研究其作用机制提供基础。 |
| 英文摘要: |
| Objective To study the main active components and pharmacological mechanisms of the anti-inflammatory function of Shuanghuanglian.Methods The chemical constituents and target proteins of "Lonicerae Japonicae Flos", "Scutellariae Radix" and"Forsythiae Fructus" were indexed by the traditional Chinese medicine system pharmacology platform (TCMSP) to construct the com?pounds-targets network. The genes related to inflammation were screened by the comparative Toxicogenomics Database (CTD) and pro?tein and protein interaction (PPI) network was constructed. Then the Gene ontology (GO) and KEGG pathway enrichment analysis wascarried out on the DAVID platform to predict the action mechanism related to anti-inflammation of Shuanghuanglian.Results 36 com? pounds and 53 potential targets were screened. The key targets were Prostaglandin G/H synthase 2(PTGS2), Prostaglandin G/H syn?thase 1(PTGS1), Nitric oxide synthase, inducible(NOS2), Transcription factor p65(RELA), RAC-alpha serine/threonine-protein kinase(AKT1). 77 GO items were obtained by GO function enrichment analysis, and 46 signaling pathways were acquired by KEGG pathway enrichment analysis.Conclusion The active ingredients and pharmacological mechanisms of Shuanghuanglian in treatment of inflam? mation were preliminarily predicted, which has provided a basis for further in-depth study on the mechanism. |
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