| 吕学宁,叶必成,王昌成.肝细胞癌中 ATP1B3基因表达及功能的生物信息学分析[J].安徽医药,2022,26(2):315-321. |
| 肝细胞癌中 ATP1B3基因表达及功能的生物信息学分析 |
| Biological analysis of ATP1B3 expression and function in hepatocellular carcinoma |
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| DOI：10.3969/j.issn.1009-6469.2022.02.026 |
| 中文关键词: 癌，肝细胞 ATP1B3 生物信息学 |
| 英文关键词: Carcinoma,hepatocellular ATP1B3 Bioinformatics |
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| 中文摘要: |
| 目的探讨 ATP1B3基因在肝细胞癌中的表达以及临床价值，预测 ATP1B3基因在肝细胞癌发生发展中的作用。方法通过 Oncomine及 TIMER数据库分析 ATP1B3 mRNA在不同肿瘤中的表达水平的差异性。利用 HCCDB、Oncomine以及 TCGA等数据库，分析 ATP1B3 mRNA在肝细胞癌和正常肝组织中的表达情况，并分析 ATP1B3的表达与临床病理特征及预后的关系。使用 GSEA法对 ATP1B3的正负相关基因进行富集分析。通过 TIMER及 GFPIA数据库分析肝细胞癌中 ATP1B3的表达与免疫浸润程度的相关性。利用 WebGestalt数据库对 ATP1B3为核心的基因网络分析预测 ATP1B3的靶向药物。结果 ATP1B3 mRNA在肝细胞癌中的表达高于正常肝组织(P<0.05)。 ATP1B3在不同肿瘤分级、分期中的表达差异有统计学意义(P <0.05)在不同性别、年龄、有无家族史、 BMI、有无血管浸润及癌组织有无周围炎症等中的表达差异无统计学意义( P >0.05)。 ATP1B3高，表达组的总生存期及疾病特异性生存期均低于低表达组( P<0.05)，且 ATP1B3高表达是影响肝细胞癌病人总体生存预后的独立危险因素( P<0.05)。对 ATP1B3正负相关基因进行富集分析得出: ATP1B3基因高表达的样本存在于核糖体、接体并影响 RNA转运、 MAPK信号通路等生物过程( P<0.01，FDR<0.05)。在肝细胞癌中 ATP1B3基因的表达与免疫浸润相关剪(cor>0，P<0.05)。 ATP1B3基因互作网络预测强心苷类药物可能是 ATP1B3的靶向药物。结论 ATP1B3可能是肝细胞癌潜在的致癌基因，其表达上调在肝细胞癌的发生发展过程发挥重要作用，并可能成为判断肝细胞癌病人预后的重要的生物学指标。 |
| 英文摘要: |
| Objective To investigate the expression and clinical value of ATP1B3 genes in hepatocellular carcinoma, and predictthe role of ATP1B3 genes in the occurrence and development of HCC.Methods The expression levels of ATP1B3 mRNA in differenttumors were analyzed using by Oncomine and TIMER database.HCCDB, Oncomine and TCGA databases were used to analyze the ex.pression of ATP1B3 mRNA in hepatocellular carcinoma and normal liver tissues. Moreover, the correlation between ATP1B3 expres.sion and clinicopathological characteristics and prognosis was analyzed. GSEA method was used to analyze the positive and negativegenes of ATP1B3.TIMER and GFPIA database were used to analyze the correlation between the expression of ATP1B3 and the degreeof immune invasion in hepatocellular carcinoma.A gene network based on ATP1B3 was constructed using GeneMANIA database andanalyzed using WebGestalt database to predict ATP1B3 targeted drugs.Results ATP1B3 mRNA expression in hepatocellular carcino. ma was higher than that in normal liver tissue (P<0.05).The expression of ATP1B3 in different tumor grades and stages was statistically different(P<0.05), there were no statistically significant differences in expression among different genders, ages, family history, BMI,vascular invasion, and surrounding inflammation in cancer tissues (P>0.05).The overall and disease free survival of the ATP1B3 high expression group was lower than that of the low expression group (P<0.05), and the high expression of ATP1B3 was an independent risk factor affecting the overall survival prognosis of HCC patients (P<0.05).Enrichment analysis of positive and negative genes related toATP1B3 lead to a natural conclusion that samples with high expression of ATP1B3 gene existed in ribosome and spliceosome and af.fected RNA transportation, MAPK signaling pathway and other biological processes (P<0.01, FDR<0.05).ATP1B3 gene expression was associated with immune infiltration in hepatocellular carcinoma (cor>0,P<0.05).ATP1B3 gene interaction network predicted that cardi. ac glycosides may be targeted drugs of ATP1B3.Conclusion ATP1B3 may be a potential oncogene in hepatocellular carcinoma, and its up-regulated expression played an important role in the development and progression of hepatocellular carcinoma, and may be an im.portant biological indicator for the prognosis of patients with hepatocellular carcinoma. |
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