| 张雅,刘晓红.hTERT和Kisspeptin在子痫前期患者血清中的表达及与子痫前期的相关性[J].安徽医药,待发表. |
| hTERT和Kisspeptin在子痫前期患者血清中的表达及与子痫前期的相关性 |
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| 投稿时间：2023-09-21 录用日期：2023-10-31 |
| DOI： |
| 中文关键词: 子痫前期 人端粒酶逆转录酶 人吻素 相关性 |
| 英文关键词: |
| 基金项目:陕西省自然科学基础研究计划项目No：2020JQ-937 |
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| 摘要点击次数: 120 |
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| 中文摘要: |
| 目的：探讨人端粒酶逆转录酶（hTERT）、人吻素（Kisspeptin）在子痫前期患者血清中的表达水平以及与子痫前期的关系。方法：选取2018年1月-2022年12月在本院就诊的300例子痫前期患者作为研究组，根据病情严重程度将其中184例轻度子痫前期患者作为轻度子痫前期组，剩余116例重度子痫前期患者作为重度子痫前期组，同时将同期来我院孕检的150例健康孕妇作为对照组。比较两组一般资料；研究组和对照组血清hTERT、Kisspeptin表达水平采用酶联免疫吸附测定法（ELISA）进行检测；检测三组血清中总蛋白、清蛋白等生化指标；Pearson法分析子痫前期患者血清hTERT、Kisspeptin水平与生化指标之间的相关性；Logistic回归分析子痫前期患者血清hTERT、Kisspeptin水平与子痫前期的关系。结果：与对照组相比，研究组患者收缩压和舒张压显著高于对照组（P<0.05）。研究组血清中hTERT、Kisspeptin水平和清蛋白水平均显著低于对照组（P<0.05），24 h尿蛋白定量、总蛋白、肌酐水平均显著高于对照组（P<0.05）。重度子痫前期组患者血清中24 h尿蛋白定量、肌酐水平显著高于轻度子痫前期组（P<0.05），血清中hTERT、Kisspeptin、清蛋白水平显著低于轻度子痫前期组（P<0.05）。子痫前期患者血清hTERT、Kisspeptin表达水平与24 h尿蛋白定量、总蛋白、肌酐呈负相关，与清蛋白呈正相关（P<0.05）。血清hTERT、Kisspeptin水平为孕妇患子痫前期的独立保护因素（P<0.05），24 h尿蛋白定量、肌酐为孕妇患子痫前期的独立危险因素（P<0.05）。结论：hTERT、Kisspeptin在子痫前期患者血清中低表达，二者与24 h尿蛋白定量、总蛋白、清蛋白、肌酐具有相关性，且均是患子痫前期的独立影响因素。通过检测血清hTERT和Kisspeptin表达水平有望为子痫前期患者的早期筛查和预测提供参考。 |
| 英文摘要: |
| Objective: To investigate the expression levels of human telomerase reverse transcriptase (hTERT) and human Kisspeptin in the serum of patients with preeclampsia and their relationship with preeclampsia. Methods: A total of 300 patients with preeclampsia who visited our hospital from January 2018 to December 2022 were included as the study group, according to the severity of the condition, 184 patients with mild preeclampsia were classified as the mild preeclampsia group, the remained 116 patients with severe preeclampsia were included in the severe preeclampsia group, meantime, 150 healthy pregnant women who came to our hospital for pregnancy examination were as the control group. The general information of the two groups was compared; enzyme linked immunosorbent assay (ELISA) was applied to detect the expression levels of hTERT and Kisspeptin in serum of the study group and the control group; biochemical indicators such as total protein and albumin in serum of three groups were detected; Pearson method was applied to analyze the correlation between serum hTERT, Kisspeptin levels and biochemical indicators in patients with preeclampsia; Logistic regression was applied to analyze the relationship between serum hTERT, Kisspeptin levels and preeclampsia in patients with preeclampsia. Results: Compared with the control group, the systolic blood pressure and diastolic blood pressure in the study group were obviously higher than those in the control group (P<0.05). The levels of hTERT, Kisspeptin, and albumin in the serum in the study group were obviously lower than those in the control group (P<0.05), while the levels of 24-hour urine protein quantification, total protein, and creatinine were obviously higher than those in the control group (P<0.05). The levels of 24-hour urine protein quantification and creatinine in the severe preeclampsia group were obviously higher than those in the mild preeclampsia group (P<0.05), while the levels of hTERT, Kisspeptin, and albumin in the serum were obviously lower than those in the mild preeclampsia group (P<0.05). The expression levels of hTERT and Kisspeptin in serum of patients with preeclampsia were negatively correlated with 24-hour urine protein quantification, total protein, and creatinine, while positively correlated with albumin (P<0.05). Serum hTERT and Kisspeptin levels were independent protective factors for preeclampsia in pregnant women (P<0.05), while 24-hour urine protein quantification and creatinine were independent risk factors for preeclampsia in pregnant women (P<0.05). Conclusion: The expression of hTERT and Kisspeptin is low in the serum of patients with preeclampsia, and they are related to 24-hour urine protein quantification, total protein, albumin and creatinine, and they are independent influencing factors of preeclampsia. The detection of serum hTERT and Kisspeptin expression levels is expected to provide reference for early screening and prediction of preeclampsia patients. |
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