| 桑珍珍.基于加权基因共表达网络分析探讨急性胰腺炎相关脓毒症潜在的关键基因[J].安徽医药,待发表. |
| 基于加权基因共表达网络分析探讨急性胰腺炎相关脓毒症潜在的关键基因 |
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| 投稿时间：2023-10-09 录用日期：2023-11-14 |
| DOI： |
| 中文关键词: 急性胰腺炎 脓毒症 关键基因 |
| 英文关键词: |
| 基金项目:河北省科技厅医学科学研究重点计划项目（182777156） |
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| 中文摘要: |
| 目的：本研究旨在识别急性胰腺炎相关脓毒症的关键基因。方法：从Gene Expression Omnibus(GEO) 数据库下载基因数据集。采用加权基因共表达网络分析(Weighted gene co-expression network analysis, WGCNA) 确定急性胰腺炎和脓毒症的枢纽模块，并将两个模块交叉以确定与急性胰腺炎相关脓毒症有关的共同基因。采用 R 软件筛选出急性胰腺炎和脓毒症的差异表达基因(Differentially expressed gene，DEG)，同时结合WGCNA和差异分析的结果，得到与急性胰腺炎和脓毒症相关的候选基因，最后将两者取交集得到急性胰腺炎相关脓毒症的关键基因。将急性胰腺炎和脓毒症的差异表达基因(DEGs)取交集并进行基因本体论(Gene ontology, GO)和京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)富集分析。结果：通过WGCNA获得了急性胰腺炎相关枢纽模块和脓毒症相关枢纽模块。将两个模块交叉，共获得161个共同基因。结合WGCNA和差异分析的结果，得出与急性胰腺炎和脓毒症相关的候选基因，通过将候选基因交叉，共筛选出11个关键基因[CRISP3、ENTPD7、ERLIN1、HK3、JAK2、KLRF1、MMP9、NEU1、PLP2、SH3GLB1、TP53I3]。根据功能富集分析，急性胰腺炎相关脓毒症的关键基因主要在免疫和炎症相关通路中增强。结论：CRISP3、ENTPD7、ERLIN1、HK3、JAK2、KLRF1、MMP9、NEU1、PLP2、SH3GLB1、TP53I3可能是参与急性胰腺炎相关脓毒症的关键基因。 |
| 英文摘要: |
| Objective: This study aimed to identify key genes involved in acute pancreatitis associated sepsis. Methods: Download the Gene dataset from the Gene Expression Omnibus(GEO) database. Weighted gene co-expression network analysis (WGCNA) was used to identify the pivotal modules of acute pancreatitis and sepsis, and the two modules were crossed to identify common genes associated with acute pancreatitis associated sepsis. Differentially expressed genes (DEG) of acute pancreatitis and sepsis were screened using R software. Combined with the results of WGCNA and differential analysis, candidate genes associated with acute pancreatitis and sepsis were obtained. Finally, the key genes of acute pancreatitis associated sepsis were obtained by intersection of the two. The differentially expressed Genes (DEGs) of acute pancreatitis and sepsis were selected and analyzed in Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) respectively. Results: Acute pancreatitis related hub modules and sepsis related hub modules were obtained through WGCNA. By crossing the two modules, a total of 161 genes were obtained in common. Combining the results of WGCNA and difference analysis, candidate genes associated with acute pancreatitis and sepsis were obtained. By crossing candidate genes, A total of 11 key genes [CRISP3, ENTPD7, ERLIN1, HK3, JAK2, KLRF1, MMP9, NEU1, PLP2, SH3GLB1, TP53I3] were screened. According to functional enrichment analysis, the key genes in acute pancreatitis associated sepsis are mainly enhanced in immune and inflammation-related pathways. Conclusion: CRISP3, ENTPD7, ERLIN1, HK3, JAK2, KLRF1, MMP9, NEU1, PLP2, SH3GLB1, TP53I3 may be the key genes involved in acute pancreatitis associated sepsis. |
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