| 张鑫,任莹,王云霞,等.高效液相色谱波长切换法同时测定银花感冒颗粒中绿原酸、甘草苷、5-O-甲基维斯阿米醇苷的含量[J].安徽医药,2018,22(7):1252-1255. |
| 高效液相色谱波长切换法同时测定银花感冒颗粒中绿原酸、甘草苷、5-O-甲基维斯阿米醇苷的含量 |
| Simultaneous determination the contents of chlorogenic acid,lquoritin and 5-O-methylvisammiosode in Yinhua Ganmao granules by HPLC with wavelength switching method |
| 投稿时间：2017-03-06 |
| DOI： |
| 中文关键词: 色谱法, 高压液相 金银花 绿原酸 甘草酸 分光光度法 5-O-甲基维斯阿米醇苷 |
| 英文关键词: Chromatography, high pressure liquid Flos lonicerae Chlorogenic acid Glycyrrhizic acid Spectrophotometry 5-O-methylvisammiosode |
| 基金项目: |
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| 摘要点击次数: 2119 |
| 全文下载次数: 695 |
| 中文摘要: |
| 目的 建立同时测定银花感冒颗粒中绿原酸、甘草苷、5-O-甲基维斯阿米醇苷的含量的方法。方法 色谱柱,Welch-C18色谱柱(4.6 mm×250 mm,5 μm),流动相:乙腈(A)-0.4%磷酸(B),梯度洗脱0～5 min(5%～13%A)；5～18 min(13%～14%A)；18～22 min(14%～20%A)；22～38 min(20%～32%A)；流速1.0 mL·min-1；柱温30 ℃,检测波长327 nm,230 nm。结果 银花感冒颗粒中绿原酸在54.05～540.50 ng(r=0.9999),甘草苷在35.58～355.80 ng(r=0.9993),5-O-甲基维斯阿米醇苷在30.59～305.90 ng(r=0.9999)范围内线性良好,平均回收率分别为99.58%(RSD为0.92%),99.28%(RSD为2.31%),98.91%(RSD为2.16%)。结论 高效液相色谱波长切换法同时测定银花感冒颗粒中绿原酸、甘草苷、5-O-甲基维斯阿米醇苷的含量具有操作简单,专属性强,准确度高的优点,可以更全面的控制银花感冒颗粒的质量。 |
| 英文摘要: |
| Objective A method was developed for simultaneous determination the contents of chlorogenic acid, Lquoritin and 5-O-methylvisammiosode in Yinhua Ganmao Granules by HPLC with wavelength switching method. Methods The chromatography separation was performed on the column of Agilent Welch-C18 (4.6 mm×250 mm, 5 μm), with acetonitrile(A)-0.4% phosphoric acid (B) as the mobile phase in the gradient elution mode (0-5 min (5%-13%A); 5-18 min (13%-14%A); 18-22 min(14%-20%A); 22-38 min (20%-32%A); at flow rate of 1.0 mL·min-1. The detection wavelength was 327 nm and 230 nm respectively, temperature of column was set at 30 ℃. Results The sample concentration of chlorogenic acid, Lquoritin and 5-O-methylvisammiosode had a good linearity with the value of peak areas at 54.05-540.50 ng (r=0.9999), 35.58-355.80 ng (r= 0.9993), 30.59-305.90 ng (r= 0.9999), respectively. The average recoveries were 99.58% (RSD=0.92%), 99.28% (RSD=2.31%) and 98.91% (RSD=2.16%), respectively. Conclusions The method of simultaneous determination the contents of chlorogenic acid, Lquoritin and 5-O-methylvisammiosode in Yinhua Ganmao Granules by HPLC with wavelength switching method which has a good advantage of simple operation, high accuracy stability and can be used for the quality control of Yinhua Ganmao Granules effectively. |
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