| 谭定春,秦惠何.2型人类表皮生长因子受体基因对结直肠癌自然杀伤细胞 92局部浸润的影响及机制[J].安徽医药,2021,25(10):2098-2101. |
| 2型人类表皮生长因子受体基因对结直肠癌自然杀伤细胞 92局部浸润的影响及机制 |
| Effect and mechanism of HER2 gene on local infiltration and Wnt/β-catenin signal of NK cells in colorectal cancer |
| |
| DOI：10.3969/j.issn.1009-6469.2021.10.044 |
| 中文关键词: 结直肠肿瘤 基因， erbB-2 受体，自然杀伤细胞 Wnt信号通路 趋化运动 NK细胞 β连环素 |
| 英文关键词: Colorectal neoplasms Genes,erbB-2 Receptors,natural killer cell Wnt signaling pathway Chemotactic move ment NK cell Beta catenin |
| 基金项目: |
|
| 摘要点击次数: 1201 |
| 全文下载次数: 421 |
| 中文摘要: |
| 目的探讨抑制 2型人类表皮生长因子受体( HER2)基因表达对结直肠癌自然杀伤细胞 92(NK92)细胞局部浸润的影响及机制。方法本研究起止时间为 2018年 5—11月。结直肠癌细胞( SW480、SW620和 HT29)细胞及人正常肠上皮细胞(NCM460)购自美国模式培养物保藏所。 Western blotting检测上述细胞中 HER2的蛋白表达。 Western blotting及 ELISE检测 HER2的特异性小干扰 RNA(si-HER2)转染 HT29细胞效率。 Transwell小室检测重组 HER2及肿瘤培养上清对 NK92细胞迁移指数的影响。 Transwell小室检测及 Western blotting检测 si-HER2对 NK92细胞迁移指数及 β-catenin表达影响。结果 3株结直肠癌细胞 HER2表达均明显高于在 NCM460细胞表达［( 0.104±0.011)、(0.141±0.015)、(0.243±0.018)比( 0.018±0.003)］。对照组 HER2蛋白及 HER2含量均明显高于 si-HER2组［( 0.467±0.046)比( 0.101±0.012)，(125.3±10.1)pg/mL比( 74.7±4.6)pg/ mL］。重组 HER2均能抑制 NK92的迁移，促进 β-catenin蛋白表达，而转染 si-HER2可提高 NK92迁移，且抑制 β-catenin蛋白表达。结论抑制 HER2基因表达可增强结直肠癌 NK92细胞局部浸润，机制与下调 Wnt/β-catenin信号有关。 |
| 英文摘要: |
| Objective To explore the mechanism of inhibiting the expression of human epidermal growth factor receptor type 2(HER2) gene on the infiltration of Natural killer cell 92 (NK92) cells in colorectal cancer.Methods The start and end time of this study was from May 2018 to November 2018. Colorectal cancer cells (SW480, SW620 and HT29) and human normal intestinal epithelial cells (NCM460) were purchased from American Type Culture Collection. The expression of HER2 protein was detected by Westernblotting. Western blotting and ELISA were used to detect the transfection efficiency of HER2-specific small interfering RNA (si-HER2)into HT29 cells. The effect of recombinant HER2 and tumor culture supernatant on NK92 cell migration index was detected by Transwell cell assay. Transwell chamber test and Western blotting test were used to detect the effect of si-HER2 on migration index and beta-catenin expression of NK92 cells.Results The expression of HER2 in 3 colorectal cancer cells was significantly higher than that in NCM460 cells [(0.018±0.003) vs. (0.104±0.011), (0.141±0.015), (0.243±0.018)]. The expression of HER2 [(0.467±0.046) vs. (0.101± 0.012)] and HER2 content [(125.3±10.1) pg/mL vs. (74.7±4.6) pg/mL] in si-HER2 group were significantly lower than those in controlgroup. Recombinant HER2 could inhibit the migration of NK92 [(1.00±0.10) vs. (0.63±0.09)], and promote β-catenin protein expression. However, transfection of si-HER2 could significantly enhance the migration of NK92 and inhibit the expression of β-catenin protein.Conclusion Inhibition of HER2 gene expression can enhance the local invasion of colorectal cancer NK92 cells, and the mechanism is related to down-regulation of Wnt/β-catenin signal. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
