| 张艳慧,付萍萍,汪安,等.酸枣仁中掺理枣仁的高效液相色谱法鉴别方法研究[J].安徽医药,2024,28(3):482-485. |
| 酸枣仁中掺理枣仁的高效液相色谱法鉴别方法研究 |
| Study on the identification of Ziziphi Mauritianac Semen in Ziziphus Jujuba Semen by high performance liquid chromatography |
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| DOI：10.3969/j.issn.1009-6469.2024.03.011 |
| 中文关键词: 酸枣仁 药物污染 理枣仁 6'''-芥子酰斯皮诺素 掺伪 鉴别 |
| 英文关键词: Ziziphi Spinosa Hu Drug contamination Z.mauritiana Lam 6'''-Sinapoylspinosin Adulteration Identify |
| 基金项目:国家药品监督管理局中药材及饮片质量控制重点实验室项目(2022GSMPAKL12，2022GSMPA-KL10)；河北省市场监督管理局科技计划项目( 2020ZD36)；甘肃省药品科研项目( 2022GSMPA0017)；兰州市科技计划项目资助( 2022-3-41) |
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| 中文摘要: |
| 目的建立测定酸枣仁和理枣仁中 6'''-芥子酰斯皮诺素含量的高效液相色谱法(HPLC)，以鉴别酸枣仁中掺伪理枣仁。方法 2020年 12月至 2022年 12月，采用 HPLC法，选用 Agela Venusil ASB C18柱( 4.6 mm×200 mm，5 μm)；以乙腈 -0.2%冰醋酸水溶液为流动相梯度洗脱，对酸枣仁及理枣仁中 6'''-芥子酰斯皮诺素的含量进行分析测定；检测波长: 335 nm；体积流量:1.0 mL/min，柱温为 30 ℃。结果 6'''-芥子酰斯皮诺素在 2.32~138.96 μg/L(r=1)内呈良好的线性关系。理枣仁平均加样回收率为 97.5%，相对标准偏差( RSD)为 1.5%。酸枣仁平均加样回收率为 97.8%，RSD为 1.7%。6'''-芥子酰斯皮诺素在酸枣仁中含量范围为 0.003 3%~0.005 1%，在理枣仁中含量范围为 0.050 6%~0.066 0%。结论建立的 6'''-芥子酰斯皮诺素 HPLC含量测定分析方法灵敏度高、专属性强、重现性好，可用于酸枣仁中掺伪理枣仁的鉴别研究。 |
| 英文摘要: |
| Objective To establish a high-performance liquid chromatography (HPLC) method for determining the content of 6'''-Si. napoylspinosin in sour jujube kernels and Li jujube kernels to identify adulterated Li jujube kernels in sour jujube kernels.Methods From December 2020 to December 2022, the content of 6''' -sinapinol in sour jujube kernels and natural jujube kernels was analyzedand determined by HPLC method with Agela Venus ASB C18 column (4.6 mm × 200 mm, 5 μm), acetonitrile 0.2% glacial acetic acidaqueous solution was selected as the mobile phase gradient elution, detection wavelength was 335nm, Volume flow rate was 1.0mL/min,column temperature was 30 ℃.Results There was a good linear relationship between 6'''-sinapinyl spironolactone in the range of 2.32-138.96 μg/L (r=1). The average sample recovery rate of jujube kernels was 97.5%, with a relative standard deviation (RSD) of1.5%. The average sample recovery rate of sour jujube kernels was 97.8%, with an RSD of 1.7%. Its content range of sour jujube ker.nels was 0.003 3% to 0.005 1%, and the content range of natural jujube kernels was 0.050 6% to 0.066 0%.Conclusion The estab. lished HPLC method has high sensitivity, strong specificity, and good reproducibility, and can be used for the identification of adulterat.ed jujube kernels in sour jujube kernels. |
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