| 刘凤珍,王荣,崔发财,等.前列腺癌病人血清人类泛素偶联酶 E2C的表达及其对 DU145细胞增殖、侵袭和转移的影响[J].安徽医药,2024,28(5):915-920. |
| 前列腺癌病人血清人类泛素偶联酶 E2C的表达及其对 DU145细胞增殖、侵袭和转移的影响 |
| Expression of UBE2C in serum of prostate cancer patients and its effect on the proliferation, invasion and metastasis of prostate cancer DU145 cells |
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| DOI：10.3969/j.issn.1009-6469.2024.05.014 |
| 中文关键词: 前列腺肿瘤 人类泛素偶联酶 E2C 诊断 侵袭 转移 |
| 英文关键词: Prostatic neoplasms UBE2C Diagnosis Invasion |
| 基金项目:国家自然科学基金项目( 82002210) |
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| 摘要点击次数: 73 |
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| 中文摘要: |
| 目的探寻人类泛素偶联酶 E2C(UBE2C)在前列腺癌病人血清中表达及对前列腺癌细胞增殖、侵袭和迁移能力的影响。方法采用基因表达交互分析数据库( GEPIA)分析 UBE2C在前列腺癌组织中的表达情况，筛选 2021年 1月至 2022年 6月在河南省人民医院住院治疗的 50例前列腺癌病人作为研究对象，另选前列腺增生病人和健康体检者各 50例作为对照，采用酶联免疫吸附( ELISA)实验检测血清 UBE2C浓度。将 si-UBE2C及 si-NC转染前列腺癌 DU145细胞，采用 CCK-8实验和 transwell实验检测细胞增殖、侵袭和迁移能力的改变，采用蛋白质印迹法检测细胞中 UBE2C、t-Akt、p-Akt、MMP-2和 MMP-9的表达。结果 GEPIA数据库中，前列腺癌组织中 UBE2C表达显著高于癌旁组织( P<0.05)；前列腺癌病人血清 UBE2C表达水平显著高于前列腺增生组和对照组［ 0.64(0.41，1.06)μg/L比 0.28(0.22，0.39)μg/L和 0.19(0.18，0.21)μg/L，P<0.05］血清 UBE2C诊断前列腺癌的曲线下面积( AUC)为 0.86，诊断灵敏度和特异度分别为 76.3%和 88.9%，血清 UBE2C联合血清前列，腺特异性抗原( PSA)诊断前列腺癌的 AUC为 0.90，诊断灵敏度和特异度分别为 82.4%和 89.7%。血清 UBE2C表达与骨转移、淋巴结转移、 gleason评分和 TNM分期相关(均 P<0.05)。转染 si-UBE2C可显著降低 DU145细胞中 UBE2C表达( P<0.05)，p-Akt、MMP-2和 MMP-9表达降低( P<0.05)，DU145细胞的增殖、侵袭和迁移能力均得到抑制［ si-NC组和 si-UBE2C组的增殖活性分别为 48 h:(0.69±0.03)和( 0.57±0.02)72 h:(0.88±0.03)和( 0.65±0.03)；细胞侵袭数量分别为( 225.7±13.6)个和( 122.7±14.6)个；细胞迁移数量分别为( 129.3±7.6)个和(，75.0±11.8)个，均 P<0.05］。结论 UBE2C在前列腺癌病人血清中表达升高并对肿瘤诊断有一定价值，沉默 UBE2C表达可显著抑制前列腺癌 DU145细胞的增殖、侵袭和转移。 |
| 英文摘要: |
| Objective To investigate the expression of human ubiquitin coupling enzyme E2C (UBE2C) in the serum of prostate can-cer (PCa) patients and its effect on the proliferation, invasion and migration of PCa cells.Methods Gene Expression Interaction Analy-sis database (GEPIA) was used to detect the expression of UBE2C in PCa tissues. 50 cases of PCa patients hospitalized in He′nan Pro-vincial People′s Hospital from January 2021 to June 2022 were selected as the research object, and 50 cases of benign Prostatic Hyper-plasia (BPH) and 50 cases of healthy subjects were selected as control groups. The expression of UBE2C in the serum of all subjectswas detected by enzyme-linked immunosorbent assay (ELISA). After transfected with si-UBE2C and si-NC, the proliferation, invasion and migration of DU145 cells were detected by CCK-8 assay and transwell assay. The expression of UBE2C, t-Akt, p-Akt, MMP-2 and MMP-9 were detected by western blot. Results In the GEPIA database, UBE2C expression in PCa tissues was significantly higher than that in adjacent normal tissues (P<0.05). The expression of UBE2C in serum of PCa patients was significantly higher than that ofBPH patients and healthy subjects [0.64 (0.41,1.06)μg/L vs. 0.28 (0.22,0.39)μg/L and 0.19 (0.18,0.21) μg/L,P<0.05] (P<0.05). The ar-ea under curve (AUC) of serum UBE2C in the diagnosis of PCa was 0.86, and the diagnostic sensitivity and specificity were 76.3% and88.9%, respectively. The AUC of serum UBE2C combined with serum prostate-specific antigen (PSA) in the diagnosis of PCa was0.900, and the sensitivity and specificity were 82.4% and 89.7%, respectively. Serum UBE2C expression in PCa patients was correlat-ed with bone metastasis, lymph node metastasis, Gleason score and TNM stage (all P<0.05). Transfected with si-UBE2C significantly decreased the expression of UBE2C in DU145 cells (P<0.05), and also decreased the expression of p-Akt, MMP-2 and MMP-9 protein (P<0.05), inhibited the proliferation, invasion and migration of DU145 cells [The proliferative activity of si-NC group and si-UBE2C group were 48 h: (0.69±0.03), (0.57±0.02), 72h: (0.88±0.03), (0.65±0.03); the number of cell invasion was (225.7±13.6) and (122.7±14.6); the number of cell migration was (129.3±7.6) and (75.0±11.8), all P<0.05].Conclusions Serum UBE2C expression is increasedin PCa patients and has potential diagnostic value. Silencing UBE2C expression can significantly inhibit the proliferation, invasion andmetastasis of PCa DU145 cells. |
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