| 杨 瑾.HPLC 法测定复方氯甲霜中氯霉素及甲硝唑的含量[J].安徽医药,2016,20(7):. |
| HPLC 法测定复方氯甲霜中氯霉素及甲硝唑的含量 |
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| 投稿时间：2016-03-16 录用日期：2016-05-30 |
| DOI：同意录用 |
| 中文关键词: 高效液相色谱法 氯霉素 甲硝唑 质量标准 |
| 英文关键词: HPLC Chloramphenicol Metronidazole Cream Quality Specification Standard |
| 基金项目: |
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| 摘要点击次数: 2401 |
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| 中文摘要: |
| 目的 对复方氯甲霜的质量标准进行再提高研究。方法 色谱柱为AgiLent XDB-C18(150mm×4.6mm,5um)；流速为1ml/min；柱温为30℃；进样量为10ul；氯霉素的流动相为甲醇-庚烷磺酸钠缓冲溶液(32:68)；紫外检测波长为277nm；甲硝唑的流动相为甲醇-水(20:80)；紫外检测波长为320nm；结果 氯霉素、甲硝唑进样量分别在5.56～111.2μg? mL?1,26.02～260.2 μg.mL?1浓度范围内与峰面积呈良好的线性关系,r分别为1.0000和0.9996(n=6)；平均回收率分别为103.8 %,99.6 %,RSD分别为1.73 %和0.43 %(n=9)。结论 本方法简便快速、准确、重复性好,可用于该制剂中氯霉素及甲硝唑的含量测定。 |
| 英文摘要: |
| Objective To improve study quality standards of Chloramphenicol Metronidazole Cream. Methods The Chloramphenicol and Metronidazole were assayed by HPLC. In this method,the AgiLent XDB-C18(150mm×4.6mm,5um) was used at the rate of 1.0 mL/min and the column temperature of 30℃. 277 nm was selected as the detection wavelength for Chloramphenicol,the mobile phase was methanol- sodium heptanesulfonate (20:80)for Chloramphenicol.320 nm was selected as the detection wavelength for Metronidazole, the mobile phase was methanol-water for Chloramphenicol.Results A good linear relationship was established between the peak response and the concentration of Chloramphenicol and Metronidazole over the range of 5.56～111.2μg• mL−1(r=1.0000) and 26.02～260.2 μg.mL−1(r=0.9996) respectively. The mean recovery of Chloramphenicol and Metronidazole was 103.8 %(RSD=1.73%), and 99.6 % (RSD=0.43%) respectively． Conclusion The research will be pursuant of quality standard of Chloramphenicol Metronidazole Cream. |
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