| 储进锦,张 帆,王 凡.辐射对人胶质瘤U251细胞COX-2和survivin表达的影响[J].安徽医药,2015,19(11):2115-2118. |
| 辐射对人胶质瘤U251细胞COX-2和survivin表达的影响 |
| The effect of radiation on the expression of COX-2 and survivin in glioma cell U251 |
| 投稿时间：2015-06-12 |
| DOI： |
| 中文关键词: 辐射 胶质瘤 COX-2 survivin 免疫组化 |
| 英文关键词: radiation glioma COX-2 survivin immunohistochemistry |
| 基金项目:安徽省自然科学基金项目(No 1308085MH134) 作者简介:储进锦,女,硕士研究生 |
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| 中文摘要: |
| 目的 研究辐射对人胶质瘤U251细胞COX-2和survivin蛋白表达的影响。方法 体外培养人胶质瘤U251细胞,随机分为实验组和对照组,实验组经过10 Gy剂量X线照射,对照组无特殊处理,培养48 h后用免疫组化法分别检测两组细胞中COX-2和survivin蛋白的表达情况。结果 COX-2和survivin蛋白在人胶质瘤U251细胞的细胞质和细胞核中表达,X线照射48 h后检测出COX-2和survivin蛋白在细胞内的表达较对照组均有明显增加(P<0.05)。结论 辐射可诱导胶质瘤细胞COX-2和survivin蛋白表达的增加,这可能是胶质瘤存在放射抵抗的机制之一。 |
| 英文摘要: |
| Objective To observe the effect of radiation on the expression of COX-2 and survivin proteins in human glioma cell line U251.Methods The human glioma cell line U251 was divided into control group(0 Gy) and experimental group(10 Gy).We detected the expression of COX-2 and survivin proteins in cells of two groups by immunohistochemical method, and observed the change of expression induced by radiation. Results The positive stainings of COX-2 and survivin proteins were mainly located in cytoplasm and nucleus of U251 cells. The expression of COX-2 and survivin in U251 cells after radiation were significantly higher than the expression without radiation (P<0.05).Conclusion The expression of COX-2 and survivin proteins are increased induced by radiation, which may become one of mechanisms about the radioresistance of glioma cells. |
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