| 许燕,赵爽,邸亮.药用植物中鲨烯环氧酶基因的研究进展[J].安徽医药,2016,20(3):417-420. |
| 药用植物中鲨烯环氧酶基因的研究进展 |
| Research progress on squalene epoxidase gene of the medicinal plant |
| 投稿时间：2015-10-17 |
| DOI： |
| 中文关键词: 鲨烯环氧酶 基因克隆 原核表达 |
| 英文关键词: squalene epoxidase gene cloning prokaryotic expression |
| 基金项目:国家自然科学基金(No 81160502)；云南省自然科学基金(No 2011FZ153)；云南省教育厅自然科学基金(No 2013J040)作者简介:许燕,女,硕士研究生 |
|
| 摘要点击次数: 2818 |
| 全文下载次数: 52 |
| 中文摘要: |
| 鲨烯环氧酶(SE)存在于内质网的微粒体中,在鲨烯转变为环氧化鲨烯的生物过程中作为生物催化剂起催化作用。而环氧化鲨烯是从鲨烯生成环阿屯醇、香树素、羊毛甾醇等的过程中重要的中间产物。SE的活性决定了环氧化鲨烯的生物合成的效率,继而,以环氧化鲨烯作为前体的各类甾体皂苷、三萜皂苷、甾醇等化合物的生物合成也随之受其影响,后续产物的产量取决于它的含量和活性。因此SE被认为是甾体皂苷生物合成途径中的一个非常重要的调控酶,已在多种植物中克隆出并进行了初步功能鉴定。该文概述几个重要药用植物的SE基因的克隆及原核表达,为从其他药用植物中克隆SE基因提供一定的参考依据及方法思路。 |
| 英文摘要: |
| Squalene epoxidase exists in the microsome of the endoplasmic reticulum,which acts as a bio-catalyst in the process of squalene turning to epoxidation squalene.Epoxidation squalene is an intermediate product generated from the process of squalene producing cycloartenol,amyrin,and lanosterol.The activity of SE determines the effficiency of the biosynthesis of epoxidation squalene,which then affects the speed to synthesize steroid saponin,triterpenoid saponin,and sterol and also the activity and quanlity of them.So SE has been taken as an important regulatory enzyme in the biosynthesis of cycloartenol,which was cloned from several plants and its basic functions were ensured.This article summarizes the cloning of SE genes in some medicinal plants and their prokaryotic expression,provides reference for SE gene cloning in other medicinal plants. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
