| 王娅,李嘉盈.桑叶抗糖尿病活性成分研究[J].安徽医药,2016,20(3):425-428. |
| 桑叶抗糖尿病活性成分研究 |
| Anti-diabetic constituents of mulberry leaf |
| 投稿时间：2015-10-18 |
| DOI： |
| 中文关键词: 桑叶 黄酮苷 抗糖尿病活性 |
| 英文关键词: mulberry leaf flavonoid glycoside anti-diabetic activity |
| 基金项目: |
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| 摘要点击次数: 2811 |
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| 中文摘要: |
| 目的 研究桑叶抗糖尿病的活性成分。方法 桑叶用80%乙醇和水提取。利用硅胶、凝胶等柱色谱及制备液相色谱进行分离,并根据理化性质和有机波谱技术鉴定了化合物的结构,进一步通过测定生物碱类化合物和黄酮苷类化合物对胰岛素抵抗的HepG2细胞葡萄糖消耗量来评价其抗糖尿病活性。结果 从桑叶中分离鉴定了8个化合物,分别为:1-脱氧野尻霉素(1),1,4-dideoxy-1,4-imino-D-arabinitol(2),山奈酚-3-O-β-D-吡喃葡萄糖苷(3),β-谷甾醇(4), 正三十四烷醇(5),oxyresveratrol(6),熊果酸(7),葡萄糖乙醇苷(8),此外化合物3能够显著提高胰岛素抵抗HepG2细胞葡萄糖消耗量。结论 化合物3具有较强的抗糖尿病活性。 |
| 英文摘要: |
| Objective To study the chemical constituents and their anti-diabetic activity from the mulberry leaf.Methods Extracted with 80% ethyl alcohol and water,the constituents were isolated by chromatography of silica gel,ODS and p-HPLC,whose structures were identified on the basis of the physical properties and spectral analysis. Furthermore,glucose consumption assays in insulin-resistance HepG2 cells were used to assay the anti-diabetic activity of flavonoid glycosides and alkaloids.Results Eight compounds were isolated from mulberry leaf and identified as 1-deoxynojirimycin(1),1,4-dideoxy-1,4-imino-D-arabinitol(2),Kaemperol -3-O- β-D- glucopyranoside(3),β-stigmasterol (4),n-tetratriacontanol (5),oxyresveratrol (6),ursolic acid (7),ethyl-β-D-glucopyranoside(8) and compound 3 significantly stimulated glucose consumption and phosphorylation of Akt in insulin resistance HepG2 cells.Conclusions Compound 3 exhibits excellent anti-diabetic activity. |
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