| 张秀侠.水飞蓟宾对局灶性脑缺血再灌注损伤大鼠保护作用的研究[J].安徽医药,2016,20(3):445-448. |
| 水飞蓟宾对局灶性脑缺血再灌注损伤大鼠保护作用的研究 |
| Protective effects of Silibinin on the rats with focal cerebral ischemia-reperfusion injury |
| 投稿时间：2015-11-05 |
| DOI： |
| 中文关键词: 水分蓟宾 脑缺血再灌注 大鼠 保护 |
| 英文关键词: silibinin ischemic-reperfusion rat protection |
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| 摘要点击次数: 2642 |
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| 中文摘要: |
| 目的 研究水飞蓟宾(SIL)对局灶性脑缺血再灌注损伤大鼠的保护作用,并探讨其可能的作用机制。方法 取112只清洁级雄性大鼠随机分为6组:假手术组、模型对照组、水飞蓟宾(100、200和400 mg·kg-1)预处理组和尼莫地平(32 mg·kg-1)预处理组,通过中动脉线栓法制备局灶性脑缺血再灌注大鼠模型。再灌注6 h后,进行神经功能评分,分析测定脑组织梗死体积及含水量；通过苏木精-尹红(HE)染色法观察脑组织形态学变化；测定血清中磷酸肌酸激酶(CPK)、乳酸脱氢酶(LDH)含量及总抗氧化能力(T-AOC)水平；测定脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量；通过TUNEL染色观察神经细胞凋亡状况并计算凋亡指数(AI),通过Western blot方法测定脑组织中NF-κB蛋白表达并进行半定量分析。结果 与模型组相比,水飞蓟宾(200和400 mg·kg-1)预处理组大鼠神经功能评分显著降低、脑梗死体积和含水量均显著降低,脑组织病理形态学变化及神经细胞凋亡均明显减轻,凋亡指数显著降低；脑组织中SOD、CAT活性显著升高且MDA含量显著降低；血清中CPK,LDH含量显著降低；脑组织中NF-κB蛋白表达量显著降低；水分蓟宾400 mg·kg-1预处理组血清中T-AOC水平显著升高；差异均具有统计学意义(P<0.05,P<0.01)。结论 水分蓟宾对局灶性脑缺血再灌注损伤大鼠具有保护作用,其作用机制可能与水分蓟宾能够改善局灶性脑缺血再灌注损伤大鼠脑组织中抗氧化酶活性、抑制氧化应激损伤有关。 |
| 英文摘要: |
| Objective To investigate the protective effects of Silibinin (SIL) on the rats with focal cerebral ischemia-reperfusion injury.Methods 112 rats were randomized into 6 groups:sham operation group,model control group,SIL 100,0 and 400 mg·kg-1 treatment groups and Nimodipine (32 mg·kg-1) treatment group; focal cerebral ischemia-reperfusion rat models were made by Zea-Longa occluding suture. 6h after reperfusion,the neurological deficits,ratio of infarct volume,water content of the brain were evaluated; the histopathological changes and neurocyte apoptosis were observed,and the AI was determined. The content of CPK,LDH and the level of T-AOC in serum were determined; the activity of SOD,CAT and the content of MDA in brain tissue were determined; the expression of NF-κB protein was detected by Western blot and semi-quantitative analysis.Results Compared with model control group,the neurological scores of SIL (200 and 400 mg·kg-1) pre-treatment groups were significantly decreased,and the ratio of infarct volume and brain water were significantly decreased; the histopathological changes and neurocyte apoptosis of SI pre-treatment groups were significantly improved and the AI was significantly decreased; the activity of SOD,CAT in brain tissue were significantly increased and the content of MDA were significantly decreased,the content of CPK,LDH in serum were significantly decreased,the expression of NF-κB protein was significantly down-regulated(P<0.05,P<0.01),and the level of T-AOC in SIL 400 mg·kg-1 pre-treatment group was significantly increased.Conclusions SIL had protective effects on the rats with focal cerebral ischemic-reperfusion injury,which was perhaps related with its pharmacological effects of enhancing the activity of antioxidant enzymes and depressing the oxidative stress injury. |
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